LOCUS       sequence-438206-        4341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for expression PzPKS2 in mammalian cells (HEK293NT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Palkina KA, Karataeva TA, Perfilov MM, Fakhranurova LI, Markina NM, 
            Somermeyer LG, Garcia-Perez E, Vazquez-Vilar M, Rodriguez-Rodriguez 
            M, Vazquez-Vilriales V, Shakhova ES, Mitiouchkina T, Belozerova OA, 
            Kovalchuk SI, Alekberova A, Malyshevskaia AK, Bugaeva EN, Guglya EB,
            Balakireva A, Sytov N, Bezlikhotnova A, Boldyreva DI, Babenko VV, 
            Kondrashov FA, Choob VV, Orzaez D, Yampolsky IV, Mishin AS, 
            Sarkisyan KS
  TITLE     A hybrid pathway for self-sustained luminescence.
  JOURNAL   Sci Adv. 2024 Mar 8;10(10):eadk1992. doi: 10.1126/sciadv.adk1992. 
            Epub 2024 Mar 8.
  PUBMED    38457503
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-03-8"; volume: "10(10):eadk1992. doi"; pages: " 
            10.1126/sciadv.adk1992. Epub 2024 Mar 8"
COMMENT     Sequence Label: pNK7184
FEATURES             Location/Qualifiers
     source          1..4341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     35..53
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(57..100)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    103..124
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     enhancer        145..448
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        449..652
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     603..623
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    1989..2110
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2026..2045)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2080..2099
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    2262..2282
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2283..2340
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2364..2381)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2535..3123)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2615..2634)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3356..4171)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3356..4171)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4079..4098
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4163..4182
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt
       61 cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct
      121 agagactagt ggaagacatg gaggcgttac ataacttacg gtaaatggcc cgcctggctg
      181 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      241 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      301 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      361 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      421 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      481 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      541 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      601 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
      661 gaaccgtcag atccgctagc gctaccggtc gccacaatgg ccccatctgt ggaagaaatc
      721 aggaaggccc agagagctca gggcccggcc actgtcctcg ccatcggcac cgccacccca
      781 cccaactgcg tctatcagaa ggactatccg gactactact tccgtgtcac taactcagag
      841 cacatgaccg aattgaagga gaagttcagg agaatgtgcg acaaatcaat gatcgagaag
      901 cgttacatgc ttttgaccga ggatcttctc aaggagaacc ccagcatgtg cgcttacatg
      961 ggatcctccc ttgacgcaag acaggacttg gtcgtcgctg aggtcccaag gctcggcaaa
     1021 gaggccgcac agaaggccat caaggagtgg ggccaaccca agtctaagat cacccacgtc
     1081 atcatgtgca ccacttcagg tgtcgacatg cctggcgcag actaccagct cactaagctt
     1141 ctcggccttc gtccttccgt caagcgattc atgatgtacc agcaaggttg cttcgctggt
     1201 ggaaccgttc ttcgactcgc taaggacgtg gctgagaaca acaagggagc acgagtcctc
     1261 gtcgtttgct cagagatcac cgccatctgt ttccgtggcc caacggacac ccacctggac
     1321 tccatggtgg gacaagcctt attcggagat ggttcaggag ccgtcatcat cggagccgat
     1381 ccagacttaa ccgtggagag gccaatcttc gagctggtct ggaccgccca gaccatcctc
     1441 ccagactccg agggagccat cgacggtcac cttagagaag tcgggttgac tctccatctc
     1501 ctcaaggacg ttccagggct tatctcaaag aacatcacca aggcactcgt ggaggccttt
     1561 aacccacttg gaatctctga ctggaacgag cttttctggg tcgcacaccc cggtggaccc
     1621 gctatccttg accaggttga ggagaaactc gctctcaagc ctgagaagat gaaggctaca
     1681 aggcaagtgc ttaatgacta cgggaacatg tcaagcgctt gtgtgctgtt tatcttagat
     1741 gagatgagga agaagtcttt ggagaacggt cattctacta caggtgaagg attggaatgg
     1801 ggtgttctct ttgggtttgg acctgggctt acggttgaga ctgtcgtgct gcacagtgtc
     1861 cctgttgtaa actcaggttg atgatcataa tcagccatac cacatttgta gaggttttac
     1921 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     1981 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     2041 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     2101 atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     2161 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     2221 aaagaataga ccgagatagg gttgagtgtc gctatgtctt ctactagtag cggccgctgc
     2281 agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga
     2341 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     2401 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2461 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2521 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2581 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2641 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2701 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2761 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2821 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2881 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2941 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     3001 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3061 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3121 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3181 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3241 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagt
     3301 cccgtcaagt cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga
     3361 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     3421 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     3481 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     3541 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     3601 ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat
     3661 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     3721 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     3781 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     3841 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag
     3901 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     3961 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     4021 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
     4081 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctgg
     4141 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     4201 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     4261 tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcagc
     4321 tcgagtgcca cttgacgtct a
//
