LOCUS       sequence-438210-       19533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level P vector carrying fungal bioluminescence 
            system lacking luciferase gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19533)
  AUTHORS   Balakireva AV, Karataeva TA, Karampelias M, Mitiouchkina TY, 
            Machacek J, Shakhova ES, Perfilov MM, Belozerova OA, Palkina KA, 
            Drazna N, Vondrakova Z, Fleiss A, Fakhranurova LI, Markina NM, 
            Morozov VV, Bugaeva EN, Delnova GM, Choob VV, Yampolsky IV, Petrasek
            J, Mishin AS, Sarkisyan KS
  TITLE     Non-invasive imaging of salicylic and jasmonic acid activities in 
            planta
  JOURNAL   bioRxiv 2025.02.17.636591; doi: 
            https://doi.org/10.1101/2025.02.17.636591
REFERENCE   2  (bases 1 to 19533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.02.17.636591; doi: https://doi.org/10.1101/2025.02.17.636591"
COMMENT     Sequence Label: pNK091
FEATURES             Location/Qualifiers
     source          1..19533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      187..894
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        3037..3382
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      4400..5107
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     terminator      7014..7266
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7336..7360
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(7483..8071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7563..7582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8242..9036)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(9037..9141)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9424..9618
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9684..10757)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11186..11815)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12079..12103
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        12287..12466
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    12497..12551
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             12553..13347
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12607..12626)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     13217..13236
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      13374..14081
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        14181..14526
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 gatttgtcag aatcattgaa cgctcttggg atggactcca tcatgttcgc acagttgaga
       61 aagaggatag gcgaagggct tggattgagt gttcctatgg tttttctgtc agacgctttc
      121 tccataggtg aaatggttag caacctggtg gaacaggctg aggcttccga ggacaactag
      181 cttgtcctgc tttaatgaga tatgcgagaa gcctatgatc gcatgatatt tgctttcaat
      241 tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct cagatcctta ccgccggttt
      301 cggttcattc taatgaatat atcacccgtt actatcgtat ttttatgaat aatattctcc
      361 gttcaattta ctgattgtac cctactactt atatgtacaa tattaaaatg aaaacaatat
      421 attgtgctga ataggtttat agcgacatct atgatagagc gccacaataa caaacaattg
      481 cgttttatta ttacaaatcc aattttaaaa aaagcggcag aaccggtcaa acctaaaaga
      541 ctgattacat aaatcttatt caaatttcaa aagtgcccca ggggctagta tctacgacac
      601 accgagcggc gaactaataa cgctcactga agggaactcc ggttccccgc cggcgcgcat
      661 gggtgagatt ccttgaagtt gagtattggc cgtccgctct accgaaagtt acgggcacca
      721 ttcaacccgg tccagcacgg cggccgggta accgacttgc tgccccgaga attatgcagc
      781 atttttttgg tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac agtgacgaca
      841 aatcgttggg cgggtccagg gcgaattttg cgacaacatg tcgaggctca gcaggaccgc
      901 tactagaatt cgagctcgga gtggcagaca tactgtccca caaatgaaga tggaatctgt
      961 aaaagaaaac gcgtgaaata atgcgtctga caaaggttag gtcggctgcc tttaatcaat
     1021 accaaagtgg tccctaccac gatggaaaaa ctgtgcagtc ggtttggctt tttctgacga
     1081 acaaataaga ttcgtggccg acaggtgggg gtccaccatg tgaaggcatc ttcagactcc
     1141 aataatggag caatgacgta agggcttacg aaataagtaa gggtagtttg ggaaatgtcc
     1201 actcacccgt cagtctataa atacttagcc cctccctcat tgttaaggga gcaaaatctc
     1261 agagagatag tcctagagag agaaagagag caagtagcct agaagtagtc aaggcggcga
     1321 agtattcagg caggtggcca ggaagaagaa aagccaagac gacgaaaaca ggtaagagct
     1381 aagcttaatg gtccaagaca cctcaagcgc tagtaccagt ccaattttga caagatggta
     1441 tatagatact agacctttga ctgcttctac cgcagccttg ccactgttgg aaactctgca
     1501 gcctgcagac cagataagtg tccaaaagta ctaccatctg aaggataagc atatgtctct
     1561 cgcatccaat cttctcaagt acttgtttgt tcacaggaat tgccgaatcc catggtctag
     1621 cattgtaata tcaagaactc cagatcccca tagaaggccc tgttatatac caccctctgg
     1681 ttctcaagag gacagcttta aggatggcta cactggaatt aatgtcgagt tcaacgtatc
     1741 ccatcaagca agcatggtgg caatagcagg cactgcattt acacccaact ctggaggtga
     1801 ttccaaactt aagccagagg ttggcataga cattacttgc gtgaatgagc gacaggggcg
     1861 aaacggtgag gagcgatccc ttgaaagttt gaggcagtac atagatattt tctcagaggt
     1921 tttttccacc gcagagatgg caaacatcag aagattggat ggggtgtcat caagctcctt
     1981 gagcgccgat aggttggtgg actatggtta ccgattgttt tacacttatt gggctcttaa
     2041 ggaagcatac atcaagatga caggggaagc cctgctggca ccatggctca gggagttgga
     2101 atttagtaat gtcgttgccc cagcagcagt ggccgaatcc ggagattccg ccggagactt
     2161 tggagagcct tataccgggg tacgaacaac cctttacaaa aacttggtcg aggatgttag
     2221 gatagaggtg gctgctctgg gaggtgacta cttgttcgcc actgctgcac gagggggcgg
     2281 tataggtgcc tccagccgac cagggggagg ccctgatggc tcaggaatta ggagtcaaga
     2341 tccctggcga ccatttaaaa agctcgacat agagagagac atacagcctt gcgccactgg
     2401 agtctgtaat tgtctgtctc gaggtagtta actagcttac cgcactgtgt gtggtttctc
     2461 aagaccaaga cagctaaagc ctaaagtcag agatctaata tgtgtattgt tattcatgac
     2521 accacagctg ccacttttgg tgttatgatc tgtttgtaga agtaggaatt cttttttttc
     2581 tacttaataa tagcttaaag agctgtgcaa tttggtctgt attttttgtg tattttgcac
     2641 tcattatttg tgaacagttt gagaactatt tattttctaa gatttgtgca cgtatgaacc
     2701 acttttcatc tatataccac catgtttatt ctgcatctat gggattgagt ttgaatattc
     2761 gttgatcaac aaagttatat ttggtggata ctacttgaag gtgcatatac tttgtgctca
     2821 tatatttagt tgatattctg gattttgagc tggacaaatt gatcaaggta gtctaatctg
     2881 gtctggttac taataaaact caagagatca ctgtctcgct ttacaggggc aactccgtcc
     2941 agagaattcg catgcggagg tcaacatggt ggagcacgac actctggtct actccaaaaa
     3001 tgtcaaagat acagtctcag aagatcaaag ggctattgag acttttcaac aaaggataat
     3061 ttcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg aaaggacagt
     3121 agaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ctatcattca
     3181 agatctctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     3241 aaaagaagag gttccaacca cgtctacaaa gcaagtggat tgatgtgaca tctccactga
     3301 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
     3361 ttcatttcat ttggagagga cacgctcgag tataagagct catttttaca acaattacca
     3421 acaacaacaa acaacaaaca acattacaat tacatttaca attatcgata caatggcacc
     3481 tatttcatcc acgtggagca ggttgataag gttcgtggct gtagaaactt ccctggttca
     3541 tattggtgag ccaattgatg caacgatgga cgttggcctc gcccgtagag aaggtaagac
     3601 aattcaagca tacgaaataa tcggaagcgg cagtgccctc gatctttctg cacaagtctc
     3661 taagaacgta ctcacagtta gggaattgtt gatgccgctc agtcgagaag agattaagac
     3721 cgttcgatgc ctcggcttga actatccggt ccacgccaca gaagctaacg tggccgtgcc
     3781 gaagtttcct aacctgttct ataagcccgt aacatcattg attggtccag gcggtcttat
     3841 tactattccc tccgtcgttc aaccccccaa ggagcaccag tcagattacg aggctgagct
     3901 ggttatagta ataggtaagg cagcaaagaa cgtatcagag gatgaggctt tggattatgt
     3961 attgggctat accgctgcaa acgatatatc tttccgtaag catcaattgg ctgtcagtca
     4021 atggagcttc agcaagggct ttgatggaac taatccactt ggtccctgct tggtttccgc
     4081 ctccagtatc cctgacccac aggatattcc aatacagtgc aaattgaatg gtggagtcgt
     4141 ccaaaatggc aacaccagag atcaaatctt caacgttaag aaaaccatat catttctttc
     4201 ccaggggact actttggagc ccggttccat tatccttacc gggaccccag atggggtggg
     4261 gttcgtacga aacccgcccc tgtatttgaa ggatggcgat gaggttatga cttggatcgg
     4321 tagtggaatc ggaactctcg caaatacagt tcgagaagaa caaacgtgct ttgcatcagg
     4381 cggccacgaa tagcttgtcc tgctttaatg agatatgcga gaagcctatg atcgcatgat
     4441 atttgctttc aattctgttg tgcacgttgt aaaaaacctg agcatgtgta gctcagatcc
     4501 ttaccgccgg tttcggttca ttctaatgaa tatatcaccc gttactatcg tatttttatg
     4561 aataatattc tccgttcaat ttactgattg taccctacta cttatatgta caatattaaa
     4621 atgaaaacaa tatattgtgc tgaataggtt tatagcgaca tctatgatag agcgccacaa
     4681 taacaaacaa ttgcgtttta ttattacaaa tccaatttta aaaaaagcgg cagaaccggt
     4741 caaacctaaa agactgatta cataaatctt attcaaattt caaaagtgcc ccaggggcta
     4801 gtatctacga cacaccgagc ggcgaactaa taacgctcac tgaagggaac tccggttccc
     4861 cgccggcgcg catgggtgag attccttgaa gttgagtatt ggccgtccgc tctaccgaaa
     4921 gttacgggca ccattcaacc cggtccagca cggcggccgg gtaaccgact tgctgccccg
     4981 agaattatgc agcatttttt tggtgtatgt gggccccaaa tgaagtgcag gtcaaacctt
     5041 gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt ttgcgacaac atgtcgaggc
     5101 tcagcaggac cgcttgtgga attcctcgag ggagtcatca aaatatttag cagcattcca
     5161 gattgggttc aatcaacaag gtacgagcca tatcacttta ttcaaattgg tatcgccaaa
     5221 accaagaagg aactcccatc ctcaaaggtt tgtaaggaag aattctcagt ccaaagcctc
     5281 aacaaggtca gggtacagag tctccaaacc attagccaaa agctacagga gatcaatgaa
     5341 gaatcttcaa tcaaagtaaa ctactgttcc agcacatgca tcatggtcag taagtttcag
     5401 aaaaagacat ccaccgagga cttaaagtta gtgggcatct ttgaaagtaa tcttgtcaac
     5461 atcgagcagc tggcttgtgg ggaccagaca aaaaaggaat ggtgcagaat tgttaggcgc
     5521 acctaccaaa agcatctttg cctttattgc aaagataaag cagattcctc tagtacaagt
     5581 ggggaacaaa ataacgtgga aaagagctgt cctgacagcc cactcactaa tgcgtatgac
     5641 gaacgcagtg acgaccacaa aagaattccc tctatataag aaggcattca ttcccatttg
     5701 aaggatcatc agatactgaa ccaatatttc tcaatggcct cttttgaaaa ttcactctct
     5761 gtgttgatcg tgggagccgg tttgggtggt ctagcagctg ctatcgctct ccgacgacag
     5821 ggccacgtgg ttaagatcta cgactcatct agctttaagg ccgagctcgg cgcaggcttg
     5881 gcagttccac cgaatacact gcgtagtcta cagcaattgg gttgtaacac tgaaaacctt
     5941 aacggcgttg ataatctctg ttttactgcc atgggatatg atgggtcagt gggcatgatg
     6001 aacaatatga ctgattaccg tgaagcatat ggcactagtt ggatcatggt tcatcgtgtc
     6061 gatttgcata acgagttgat gagagttgct ttggacccag ggggattggg ccctcccgca
     6121 acgcttcacc tgaatcatcg agtcacgttt tgtgatgtag atgcttgtac ggtgacattt
     6181 accaacggta caactcaaag tgcagatctt atcgttgggg ctgacgggat acgttcaact
     6241 atccgtagat ttgtcttgga ggaggacgtg accgttcccg caagcggaat tgtggggttt
     6301 cgatggttgg tacaggctga tgccctcgat ccatatcctg aactcgattg gatagtcaaa
     6361 aagccaccgc tcggagctag gttgatttct acgccccaga atccacaatc tggagtggga
     6421 ctggcagatc gtagaactat tattatatac gcttgtcgtg gggggacaat ggtaaatgtg
     6481 ctggccgtac acgatgacga gcgagatcag aatactgcag attggagcgt tccagcatca
     6541 aaggatgact tgtttcgagt attccatgac taccatccga gattcagacg gctcttggaa
     6601 ttggctcaag atataaatct ctggcagatg agggttgtgc cggtgctcaa gaaatgggtg
     6661 aacaaacgag tgtgcctgct tggagatgcc gctcatgcct ctcttccgac tctcggccag
     6721 ggtttcggga tgggattgga ggacgccgta gcccttggta ctctcttgcc aaaaggtacg
     6781 actgctagtc aaatagagac aagactggct gtctacgaac agcttagaaa ggaccgagca
     6841 gagtttgtag ccgctgaaag ttatgaagag cagtatgtcc ccgagatgag gggcttgtac
     6901 ttgcgtagca aggaacttag agatagagtc atggggtatg acataaaagt ggagtctgag
     6961 aaggtactgg aaaccttgct taggagctct aactcagctt agcttgtcaa gcagatcgtt
     7021 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta
     7081 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
     7141 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
     7201 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
     7261 tagatcgacg ctgagcttgt cttcgaggat gcacatgtga ccgagggaca cgaagtggtt
     7321 taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta
     7381 ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     7441 tgccaaccac aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7501 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7561 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7621 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7681 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7741 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7801 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7861 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7921 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7981 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8041 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8101 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8161 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8221 tgagtaaact tggtctgaca gctaaaacaa ttcatccagt aaaatataat attttatttt
     8281 ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc
     8341 gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc
     8401 gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc
     8461 tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc
     8521 atacagctcg cgcggatctt taaatggagt gtcctcttcc cagttttcgc aatccacatc
     8581 ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt
     8641 atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc
     8701 gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc
     8761 ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga cctttggaac
     8821 aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt
     8881 ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt
     8941 atatacctta gcaggagaca ttccttccgt atcttttgcg cagcggtatt tttcgatcag
     9001 ttttttcaat tccggtgata ttctcatttt agccatactc ttcctttttc aatattattg
     9061 aagcatttat caaggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9121 taaacaaata ggggttccgc gcacgaattg gccagcgctg ccatttttgg ggtgaggccg
     9181 ttcgcggccg aggggcgcag cccctggggg gatgggagct cggtgccttg atgtgggcgc
     9241 cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg
     9301 ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg
     9361 gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac
     9421 agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg
     9481 gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg
     9541 tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca
     9601 atgtacgtgc tatccacagg aaagaagctt tttcgacctt tttcccctgc tagggcaatt
     9661 tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg
     9721 cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc
     9781 ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg
     9841 gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg
     9901 gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg
     9961 gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca
    10021 gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg
    10081 ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta
    10141 cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc
    10201 tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac
    10261 acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg
    10321 gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg
    10381 gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca
    10441 cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg
    10501 tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc
    10561 gacgacgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct
    10621 tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg
    10681 gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat
    10741 ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc
    10801 ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt
    10861 ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa
    10921 ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat
    10981 agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc
    11041 tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac
    11101 gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg
    11161 ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg
    11221 cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg
    11281 cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt
    11341 acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca
    11401 cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca
    11461 ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt
    11521 cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta
    11581 cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga
    11641 caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat
    11701 cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg
    11761 cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt
    11821 ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa
    11881 gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc
    11941 caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag
    12001 ccgcacggct gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
    12061 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
    12121 acttaataac acattgcgga cgtttttaat gtactggggt ggatgcagtg ggccccactc
    12181 tgtggaagac aatgccgaat tcggatccgg agcggagaat taagggagtc acgttatgac
    12241 ccccgccgat gacgcgggac aagccgtttt acgtttggaa ctgacagaac cgcaacgttg
    12301 aaggagccac tgagccgcgg gtttctggag tttaatgagc taagcacata cgtcagaaac
    12361 cattattgcg cgttcaaaag tcgcctaagg tcactatcag ctagcaaata tttcttgtca
    12421 aaaatgctcc actgacgttc cataaattcc cctcggtatc caattagagt ctcatattca
    12481 ctctcctatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattaca
    12541 tttacaatta ccatggttga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
    12601 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
    12661 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
    12721 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
    12781 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
    12841 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
    12901 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
    12961 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
    13021 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
    13081 cgcatgcccg acggcgagga tctcgtcgtg actcatggcg atgcctgctt gccgaatatc
    13141 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
    13201 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
    13261 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
    13321 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgctaga gtcctgcttt
    13381 aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct gttgtgcacg
    13441 ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg ttcattctaa
    13501 tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt caatttactg
    13561 attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt gtgctgaata
    13621 ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt tttattatta
    13681 caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg attacataaa
    13741 tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc gagcggcgaa
    13801 ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg tgagattcct
    13861 tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc aacccggtcc
    13921 agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt tttttggtgt
    13981 atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg
    14041 gtccagggcg aattttgcga caacatgtcg aggctcagcc gctgcaagaa ttcaagcttg
    14101 gaggtcaaca tggtggagca cgacactctg gtctactcca aaaatgtcaa agatacagtc
    14161 tcagaagatc aaagggctat tgagactttt caacaaagga taatttcggg aaacctcctc
    14221 ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc
    14281 tcctacaaat gccatcattg cgataaagga aaggctatca ttcaagatct ctctgccgac
    14341 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agaggttcca
    14401 accacgtcta caaagcaagt ggattgatgt gacatctcca ctgacgtaag ggatgacgca
    14461 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
    14521 aggacacgct cgagtataag agctcatttt tacaacaatt accaacaaca acaaacaaca
    14581 aacaacatta caattacatt tacaattatc gatacaatga actcaagtaa gaatccgccc
    14641 tccacattgt tggatgtatt tctcgacacg gctagaaatc ttgataccgc tagtaggaat
    14701 gtcctggaat gcggggaaca tcgttggtca tatcgtgaat tggatacggt aagctctgcc
    14761 ctggcccagc accttcgata taccgtcggc ttgagtccta cagtagccgt gatcagtgag
    14821 aaccaccctt acatattggc cttgatgttg gccgtttgga aattgggggg taccttcgct
    14881 ccaatcgacg tacatagtcc cgccgagctc gtggctggca tgttgaatat agttagcccc
    14941 agctgtctgg ttataccttc tagtgacgtt accaaccaga cattggcctg cgatctcaat
    15001 ataccagtag tagctttcca ccctcatcaa tcaacgatac cggaactgaa caaaaagtac
    15061 ttgactgaca gtcaaatatc acctgacttg cctttccccg accccaacag gccggctctt
    15121 tatctgttta ccagcagcgc tacttctcgt tctaatctga aatgcgtccc cttgacgcat
    15181 acatttatcc ttagaaactc tctttcaaaa agggcctggt gcaaacgtat gaggcccgaa
    15241 accgattttg atggtattcg tgtactcggt tgggctcctt ggtcccacgt gcttgcacat
    15301 atgcaggaca ttggaccatt gacgcttttg aacgcagggt gctacgtttt tgctacgact
    15361 ccttcaacat atccgacaga gctgaaggac gatcgagatg tgatctcctg tgccgccaac
    15421 gcagtcatgt ataagggagt taagtcattt gcctgtctac cttttgtgtt gggcggactt
    15481 aaagcactct gcgaaagcga gccatctgta aaagctcagt tgcaagtgga agagcgagca
    15541 caattgctca aatccctgca gcatatgaag atcctggagt gcggtggtgc catgttggaa
    15601 gtctccgttg catcctgggc aatagagaac agaatcccca taagcattgg gataggcatg
    15661 actgaaactg gtggagccct cttcgctgga ccagttcagg caatacaaac aggattttca
    15721 tcagaggaca agttcataga ggacgccact tatcttttgg tgaaggatga ctatgagtcc
    15781 cacgccgagg aagatattaa cgaaggtgaa ctcgtcgtca agtccaggat gctgcctcgt
    15841 gggtatctgg ggtataatga tccatcattc agcgttgatg atgcaggctg ggtgacgttc
    15901 aaaacgggag ataggtacag tgttacaccc gacggcaaat ttagctggct ggggagaaat
    15961 accgacttca ttcagatgac atctggtgaa accttggacc ctcgtccgat agaaagtctc
    16021 ctctgtgagt cctcacttat ttctagggcc tgcgtaattg gcgataagtt tcttaacggg
    16081 ccagcaactg ctgtttgtgc cataatcgaa ctggaaccaa ccaccgttga gaagggtcaa
    16141 gctcattcca gagacatagc taggatattc gcccccatca acagggattt gcccccccct
    16201 ttgcgtatcg catggtcaca tgtcctggtt ttgcagccta gtgagaagat acctatgaca
    16261 aagaagggga cgatttttcg aaaaaagatc gaacaagtct ttgggtccgc attgggtgga
    16321 tcatccggcg acaatagcca agctactact gacgctagcg tggtaagacg tgacgaactc
    16381 agtaacacag tgaagcacat tatatcccga gtgttggggg tgtctgatga cgaactcttg
    16441 tggaccctct ctttcgccga gttgggtatg acttctgctt tggccaccag gattgcaaat
    16501 gagttgaacg aagtccttgt gggagtgaac ttgcctataa acgcctgcta tattcatgtg
    16561 gatcttccga gcctcagtaa tgcagtctat gccaaactcg cacacttgaa gttgccagac
    16621 cgtactccgg aaccacgaaa ggcaccagtt gaaaatcccg gaggtaaaga gatcgtcatt
    16681 gtaggccagg ccttccgttt gcccgggagt atcaacgacg tcgcctctct tagggatgcc
    16741 tttttggcta ggcaggccag ctcaattatt accgagatac ctccggacag gtgggaccac
    16801 gcatccttct acccaaaaga tattcgtttc aataaggctg ggctggtgga catcgccaac
    16861 tacgatcatt ccttctttgg gctgaccgcc acggaagcac tctatctcag ccctacgatg
    16921 cgactcgcct tggaggtgtc atttgaagca ttggagaatg ccaacattcc agtttctcaa
    16981 ctgaagggct cccagacagc agtgtatgtt gcaacaacag acgatggttt tgaaacgttg
    17041 ctcaacgccg aagcaggtta cgatgcctac accagatttt acgggactgg gagggctgcc
    17101 tctacggcat caggccgtat aagttatctc ctcgatgtac atggaccatc catcactgtg
    17161 gacacagcct gcagtggagg agcagtgtgc atagatcaag ccattgatta tctccagtcc
    17221 tcaagtgctg ctgataccgc tattatttgt gctagtaata cccattgctg gccggggtca
    17281 tttatgtttc tcagcgctca aggtatggtt tcatctgggg gtagatgtgc tacattcacg
    17341 actgatgcag acggatatgt tccatccgaa ggagctgtag ctttcatact taagacaaga
    17401 gaggctgcta tgagagataa agataccata ttggcaacaa taaaggctac gcaaatttcc
    17461 cacaacggac gatcccaagg actcgtggct cctaacgtta atagtcaagc cgatctgcat
    17521 cgtagcctcc ttcagaaagc cgggctttcc cctgccgata tacatttcat agaagcacac
    17581 ggcactggga caagtctcgg agacttgtct gaaatccaag ccattaacga cgcttatacc
    17641 tctagtcaac ccagaacagc cggaccattg attgtgtccg cctccaaaac tgtgatcggg
    17701 cacactgaac ctgctggacc attggtcggt atgctctctg tactcaattc ctttaaggaa
    17761 ggggctgtcc cggggcttgc ccaccttacc gccgacaact tgaatccggc tttggattgc
    17821 tctagtgtcc ctttgttgat tccgtatcaa ccggtacatt tggctgcacc caagccacat
    17881 agggccgctg ttatgagtta tgggttctcc ggtacactgg gagggatcgt gttggaagct
    17941 ccggatgaag agcgtcttga agaagaaccc ccaaacgaca agccaatgtt gtttgtagtt
    18001 agtgctaaga cccataccgc cctgatcgag tatttgggta gatatttgga gtttttgctt
    18061 caagccaacc cgcaagattt ctgcgacata tgttacacat catgcgtcgg ccgtgaacat
    18121 tataggtacc gtttcgcttg cgtcgccaac gacatggagg acctgatcgg ccaacttcaa
    18181 aaaaggctgg ggtccaaggt accgccaaaa ccgagctata agcgaggcgc acttgctttt
    18241 gcattctccg gtcaaggtac tcagtttaga ggtatggcaa ctgaactggc aaaagcctac
    18301 tcaggtttta ggaagatagt cagtgacttg gctaagaggg cctccgaact ctccggccat
    18361 gctattgata ggttcttgtt ggcttatgat attggcgctg aaaatgtggc accagactca
    18421 gaagcagacc aaatatgcat attcgtgtac caatgcagcg tgctcagatg gcttcagacc
    18481 atgggcattc gtcctagtgc tgtaataggt cactcactgg gagaaatctc tgcttcagtt
    18541 gcagcagggg cactcagtct cgacagcgcc ctggatctcg tgataagcag ggctagattg
    18601 ttgagatctt ctactaatgc ccccgctggt atggctgcca tgtctgctag ccaggatgag
    18661 gtcgtggaac tcatcggaaa attggacctt gataaagcta actccttgtc agtctcagtc
    18721 atcaatggcc cccagaacac cgttgtgtcc ggtagctcag cagccattga atcaatagta
    18781 gcattggcca agggtaggaa aattaaagca agcgctctta atattaatca ggcatttcac
    18841 tccccatatg ttgattctgc agtaccgggc ttgcgagcct ggtcagaaaa gcatatctca
    18901 tccgcaaggc cgctccagat accgttgtat tcaaccttgc ttggtgctca agtcagcgaa
    18961 gggcaaatgc ttaatccgga tcactgggta gatcatgccc gtaagcctgt tcagtttgct
    19021 caagccgcaa caatcatgaa agagtccttt actggggtga tcatcgacat cggtccccag
    19081 gtggttgcct ggtcactgct cttgagcaac ggtttgacat ccgtcactgc actcgccgct
    19141 aagcgtgggc gaagccaaca ggtcgctttc ctcagcgcct tggccgatct gtatcaagac
    19201 tatggtgtag tacctgactt tgtcgggttg tacgcccagc aggaggacgc tagcaggctt
    19261 aaaaagacag atattttgac ctacccgttt caaagggtca gacgatatcc gtctttcatt
    19321 ccgtcacgtc gtgcaccgac acatgcccac gttcaggacg aggagactct gtcatccggg
    19381 agcagtacgc ccacattgga aaataccgac cttgattctg gtaaggagag tctcatggga
    19441 ccgacaagag ggttgttgcg tgtcgacgac ttgagggact ccatcgttag ttctgtgaag
    19501 gacgtactcg aactcaaatc caacgaggac ctc
//
