LOCUS       sequence-438218-        5180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for expression HmS in mammalian cells (HEK293NT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Palkina KA, Karataeva TA, Perfilov MM, Fakhranurova LI, Markina NM, 
            Somermeyer LG, Garcia-Perez E, Vazquez-Vilar M, Rodriguez-Rodriguez 
            M, Vazquez-Vilriales V, Shakhova ES, Mitiouchkina T, Belozerova OA, 
            Kovalchuk SI, Alekberova A, Malyshevskaia AK, Bugaeva EN, Guglya EB,
            Balakireva A, Sytov N, Bezlikhotnova A, Boldyreva DI, Babenko VV, 
            Kondrashov FA, Choob VV, Orzaez D, Yampolsky IV, Mishin AS, 
            Sarkisyan KS
  TITLE     A hybrid pathway for self-sustained luminescence.
  JOURNAL   Sci Adv. 2024 Mar 8;10(10):eadk1992. doi: 10.1126/sciadv.adk1992. 
            Epub 2024 Mar 8.
  PUBMED    38457503
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-03-8"; volume: "10(10):eadk1992. doi"; pages: " 
            10.1126/sciadv.adk1992. Epub 2024 Mar 8"
COMMENT     Sequence Label: pNK7179
FEATURES             Location/Qualifiers
     source          1..5180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    837..958
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(874..893)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     928..947
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(965..1420)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1102..1123)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1314..1333
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1447..1551
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1553..1910
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1553..1744
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1576..1596)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1761..1896
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1945..2739
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1945..2739
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1999..2018)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2609..2628
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2927..2946)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2971..3018
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3347..3935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3836..3855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4110..4413
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4414..4617
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4568..4588
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4644..4653
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gccaaagacc ttgccgaaaa taacaaggga gctcgtgtat tggtggtttg ttctgagatg
       61 acagtgatta actttcgtgg ccccagtgag gcccacatgg attcccttgt cggacaatct
      121 cttttcggcg acggcgcctc agccgtaatc gtaggttcag atcccgatct gtcaacagag
      181 catccacttt accagattat gtccgcctcc caaatcattg tagccgactc cgaaggcgct
      241 attgatggtc acttgcgtca agagggtctt acctttcatc tgcgtaagga tgttccttct
      301 cttgtctccg ataacatcga gaatactctg gtggaagcat ttactcccat cctgatggac
      361 agtatcgact caatcattga ttggaactct atcttttgga ttgctcaccc aggtggtcct
      421 gccatcctga atcaggttca agcaaaagta ggactgaagg aggaaaaact gcgtgtgagt
      481 cgtcacattt tgtcagaata tggcaatatg tcatccgcat gcgtcttttt tatcatggat
      541 gagatgcgta aacgttccat ggaagaggga aaaggaacta ccggcgaggg ccttgagtgg
      601 ggagtgcttt ttggcttcgg tcctggtttt acggtcgaaa ccattgtgtt gcacagtgtg
      661 cccatttgaa agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggat
      721 ctagataact gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
      781 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
      841 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      901 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac
      961 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     1021 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     1081 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     1141 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     1201 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     1261 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     1321 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     1381 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     1441 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1501 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     1561 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     1621 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     1681 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     1741 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     1801 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     1861 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     1921 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     1981 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     2041 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     2101 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     2161 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     2221 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     2281 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     2341 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     2401 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     2461 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     2521 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     2581 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     2641 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     2701 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     2761 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     2821 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     2881 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     2941 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     3001 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     3061 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     3121 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     3181 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3241 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3301 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3361 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3421 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3481 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3541 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3601 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3661 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3721 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3781 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3841 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3901 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3961 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4021 ctgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca
     4081 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     4141 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     4201 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     4261 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     4321 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     4381 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     4441 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     4501 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     4561 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
     4621 ttagtgaacc gtcagatccg ctagccacca tggctaccaa aagtgtcgcc gttgaagaaa
     4681 tgtgcaaagc ccaaaaagcc ggaggccctg caacaatcct tgcaattggt actgctgtgc
     4741 ccagtaactg ctactatcag agtgaatacc ctgatttcta cttccgtgtc acgaaaagtg
     4801 atcaccttac ggacctgaaa agtaagttta aacgtatgtg cgaacgttca tccatcaaaa
     4861 agcgttatat gcacctgaca gaggagattc ttgaagaaaa tcctaatatg tgcacgtttg
     4921 cagctccatc tatcgatggc cgtcaggaca ttgtagttaa ggagatccct aagttggcta
     4981 aggaggcagc aagtaaagct atcaaggaat ggggccaacc taagtcaaat atcacccatc
     5041 ttgtcttttg tacaacttca ggtgtggaca tgcccggttg tgattatcaa ttgacgcgtc
     5101 ttttgggact tcgtccatct attaaacgtc ttatgatgta tcagcagggc tgccatgctg
     5161 gtggaaccgg cttgcgtttg
//
