LOCUS       sequence-438220-        4313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo-compatible Level 0 vector carrying promoter pWRKY70.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Balakireva AV, Karataeva TA, Karampelias M, Mitiouchkina TY, 
            Machacek J, Shakhova ES, Perfilov MM, Belozerova OA, Palkina KA, 
            Drazna N, Vondrakova Z, Fleiss A, Fakhranurova LI, Markina NM, 
            Morozov VV, Bugaeva EN, Delnova GM, Choob VV, Yampolsky IV, Petrasek
            J, Mishin AS, Sarkisyan KS
  TITLE     Non-invasive imaging of salicylic and jasmonic acid activities in 
            planta
  JOURNAL   bioRxiv 2025.02.17.636591; doi: 
            https://doi.org/10.1101/2025.02.17.636591
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.02.17.636591; doi: https://doi.org/10.1101/2025.02.17.636591"
COMMENT     Sequence Label: pNK3625
FEATURES             Location/Qualifiers
     source          1..4313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(848..952)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1020..1038
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3218..3235)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3389..3977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3469..3488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4148..4313,1..626))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac
       61 gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag
      121 gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc
      181 gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca
      241 atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca
      301 aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg
      361 gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg
      421 tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa
      481 tcaatatcgc tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc
      541 agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat
      601 acttcggcga tcaccgcttc cctcatgatg tttaactttg ttttagggcg actgccctgc
      661 tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc
      721 tgcttggatg cccgaggcat agactgtacc ccaaaaaaaa acagtcataa caagccatga
      781 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
      841 agcgcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      901 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
      961 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     1021 ataggcgtat cacgaggccc tttcgtccac tgaagagcca cttcgtggtc tcacattttg
     1081 ttagttttga ggaagttttt ggtgagtggt atcttatttt tctttcctct tggtgtggtt
     1141 tgtatgatta aaggaaagaa gagaagtgtg tgtaaatata aggtggaaat aagatttgta
     1201 atgatgggca ctttttattt tgttggtttc tttgattttt ttttttagag gaagatgcga
     1261 ggaaatgaga aggaagaaag agagatgtgt gaggaaatga gatggaactt gaacttatcc
     1321 acccatttct tttgcttatg gtgagcttga cgtaagctca aatgacgtag tgatgaatga
     1381 tgtcatttca tccctaagtg agaatggaat atatataatt ttgctgaaat ccaaacttag
     1441 ggtcctttaa tgttttctat aaccggataa aatttgtatc aaaaactaga ctaattaaaa
     1501 gagttatgaa aaactatcca tttaaagatt aaacaagatt ctaaatttca tttgtaaact
     1561 tttttgttcc tcgagattgt actaccaatt tttttttact gatgtagtaa gatcagttgg
     1621 aatagtgaat tatttatttg aaatctcgag ttaaatttga tatccaaatg atatgatccg
     1681 tttttctatg aagataattt tgaatagtag tttatatatc aaaaattcaa aaagacatgt
     1741 atatagtcct tatcagctat caatccagat gtataccccc taaatagacc tactatttca
     1801 aatagataac gagacacgaa atttacaatc aactagtccg aaatgaattg cccttgtaat
     1861 caaatacttg aatgacagat ataattggga gttgggacgg attatctaaa tcagattgat
     1921 tacgttgaaa gaatcaatat ataaactaat taactctttt ttttttgtta agttagacta
     1981 gtaaacgtat cacctcgctg cactaatata taaactatat ggagtatcaa agctaaactt
     2041 ttctacccaa tttttttata ggaacctatc ttatattata cttaaattta acatattcta
     2101 taaaaatagc aacacaatct atcgttgaaa agtattttct ttcacccgga aacactttat
     2161 tccgttttcg tggaaaaggt agagttgggc ggggcattga tttaaaactt taatattaga
     2221 agattattta aagaggtgta tgtggtcgat cgactcaatg gcccctaggc cctagctatc
     2281 tcaatgcata catgcgatgc ggatgggttc tcctcgctta gagatgtcta gttggtctat
     2341 agtgctccct cacagcctca ctctcttcta tatgcaccaa gccaccaagg tttttttaat
     2401 gatacattta tgcccaagta taaattaaca ttggtgatat tttttttaga tatgacaagt
     2461 ttttgcatac ccattatcaa aaagtcggat aatagtccat aaatttatct tacggaagca
     2521 ttataagaga atgttaaatt attacatttt ttcacgtcga acttttaaat taatgatttt
     2581 gaaatttttt cccttcatac gaagtctatt aacgccagtt gagctaggat catatcaatc
     2641 aatttcagaa cacataaaca attgcatggg ttaagaatcg acaaagtatt acttttttgt
     2701 gtataaagat aaattatgac taaaaagata cttaatccgt tttaaaatat aagatgttta
     2761 actaaaaaca cgtatattaa aaattacata tttttaaaaa gttcaaccaa acataaagaa
     2821 aatgcataat ataactaata actaatcata aaatattgca taagaacttg aaaatattac
     2881 tttattatga aactatttgt ttttttttcc aaaacatctc gtactataaa atggaaagaa
     2941 tatagtttag gataagattt aagttatttc cgggatagta ccacttaact tcagaccaaa
     3001 tttgtacgca ggttcataaa tgagatccgt tattaatgcc gtaaagttta gtggttgtgt
     3061 ctcaactttt tcattaattc aaaaaggttg gctctctagc ctaagttaat atgcgagata
     3121 ctatgataaa ccagttgctc ctgagaccac agagtgatta atgaatcggc caacgcgcgg
     3181 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     3241 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3301 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3361 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3421 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3481 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3541 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3601 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3661 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3721 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3781 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3841 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3901 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3961 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4021 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4081 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4141 ctgacagtta tttgccgact accttggtga tctcgccttt tacgtaatgg acaaattctt
     4201 ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag
     4261 cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag tcg
//
