LOCUS       sequence-438283-        4520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for expression PzPKS2 in yeast (P.pastoris).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Palkina KA, Karataeva TA, Perfilov MM, Fakhranurova LI, Markina NM, 
            Somermeyer LG, Garcia-Perez E, Vazquez-Vilar M, Rodriguez-Rodriguez 
            M, Vazquez-Vilriales V, Shakhova ES, Mitiouchkina T, Belozerova OA, 
            Kovalchuk SI, Alekberova A, Malyshevskaia AK, Bugaeva EN, Guglya EB,
            Balakireva A, Sytov N, Bezlikhotnova A, Boldyreva DI, Babenko VV, 
            Kondrashov FA, Choob VV, Orzaez D, Yampolsky IV, Mishin AS, 
            Sarkisyan KS
  TITLE     A hybrid pathway for self-sustained luminescence.
  JOURNAL   Sci Adv. 2024 Mar 8;10(10):eadk1992. doi: 10.1126/sciadv.adk1992. 
            Epub 2024 Mar 8.
  PUBMED    38457503
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-03-8"; volume: "10(10):eadk1992. doi"; pages: " 
            10.1126/sciadv.adk1992. Epub 2024 Mar 8"
COMMENT     Sequence Label: pNK7185
FEATURES             Location/Qualifiers
     source          1..4520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        452..928
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris 
                     glyceraldehyde-3-phosphate dehydrogenase"
     CDS             2133..2162
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2178..2195
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2275..2520
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        2535..2948
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2955..3002
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3021..3395
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      3461..3708
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3477..3495)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     rep_origin      complement(3783..4371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3863..3882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acctaaacgc ttaatgcctc agacgtttta atgcctctca acacctccaa ggttgctttc
       61 ttgagcatgc ctactaggaa ctttaacgaa ctgtggggtt gtagacagtt tcaggcgtgt
      121 cccgaccaat atggcctact agactctctg aaaaatcaca gttttccagt agttccgatc
      181 aaattaccat cgaaatggtc ccataaacgg acatttgaca tccgttcctg aattatagtc
      241 ttccaccgtg gatcatggtg ttcctttttt tcccaaagaa tatcagcatc ccttaactac
      301 gttaggtcag tgatgacaat ggaccaaatt gttgcaaggt ttttcttttt ctttcatcgg
      361 cacatttcag cctcacatgc gactattatc gatcaatgaa atccatcaag attgaaatct
      421 taaaattgcc cctttcactt gacaggatcc ttttttgtag aaatgtcttg gtgtcctcgt
      481 ccaatcaggt agccatctct gaaatatctg gctccgttgc aactccgaac gacctgctgg
      541 caacgtaaaa ttctccgggg taaaacttaa atgtggagta atggaaccag aaacgtctct
      601 tcccttctct ctccttccac cgcccgttac cgtccctagg aaattttact ctgctggaga
      661 gcttcttcta cggccccctt gcagcaatgc tcttcccagc attacgttgc gggtaaaacg
      721 gaggtcgtgt acccgaccta gcagcccagg gatggaaaag tcccggccgt cgctggcaat
      781 aatagcgggc ggacgcatgt catgagatta ttggaaacca ccagaatcga atataaaagg
      841 cgaacacctt tcccaatttt ggtttctcct gacccaaaga ctttaaattt aatttatttg
      901 tccctatttc aatcaattga acaactatca attcgaagag gaattcatgg ccccatctgt
      961 ggaagaaatc aggaaggccc agagagctca gggcccggcc actgtcctcg ccatcggcac
     1021 cgccacccca cccaactgcg tctatcagaa ggactatccg gactactact tccgtgtcac
     1081 taactcagag cacatgaccg aattgaagga gaagttcagg agaatgtgcg acaaatcaat
     1141 gatcgagaag cgttacatgc ttttgaccga ggatcttctc aaggagaacc ccagcatgtg
     1201 cgcttacatg ggatcctccc ttgacgcaag acaggacttg gtcgtcgctg aggtcccaag
     1261 gctcggcaaa gaggccgcac agaaggccat caaggagtgg ggccaaccca agtctaagat
     1321 cacccacgtc atcatgtgca ccacttcagg tgtcgacatg cctggcgcag actaccagct
     1381 cactaagctt ctcggccttc gtccttccgt caagcgattc atgatgtacc agcaaggttg
     1441 cttcgctggt ggaaccgttc ttcgactcgc taaggacgtg gctgagaaca acaagggagc
     1501 acgagtcctc gtcgtttgct cagagatcac cgccatctgt ttccgtggcc caacggacac
     1561 ccacctggac tccatggtgg gacaagcctt attcggagat ggttcaggag ccgtcatcat
     1621 cggagccgat ccagacttaa ccgtggagag gccaatcttc gagctggtct ggaccgccca
     1681 gaccatcctc ccagactccg agggagccat cgacggtcac cttagagaag tcgggttgac
     1741 tctccatctc ctcaaggacg ttccagggct tatctcaaag aacatcacca aggcactcgt
     1801 ggaggccttt aacccacttg gaatctctga ctggaacgag cttttctggg tcgcacaccc
     1861 cggtggaccc gctatccttg accaggttga ggagaaactc gctctcaagc ctgagaagat
     1921 gaaggctaca aggcaagtgc ttaatgacta cgggaacatg tcaagcgctt gtgtgctgtt
     1981 tatcttagat gagatgagga agaagtcttt ggagaacggt cattctacta caggtgaagg
     2041 attggaatgg ggtgttctct ttgggtttgg acctgggctt acggttgaga ctgtcgtgct
     2101 gcacagtgtc cctgttgtaa actcataatc tagaacaaaa actcatctca gaagaggatc
     2161 tgaatagcgc cgtcgaccat catcatcatc atcattgagt ttgtagcctt agacatgact
     2221 gttcctcagt tcaagttggg cacttacgag aagaccggtc ttgctagatt ctaatcaaga
     2281 ggatgtcaga atgccatttg cctgagagat gcaggcttca tttttgatac ttttttattt
     2341 gtaacctata tagtatagga tttttttgtc attttgtttc ttctcgtacg agcttgctcc
     2401 tgatcagcct atctcgcagc tgatgaatat cttgtggtag gggtttggga aaatcattcg
     2461 agtttgatgt ttttcttggt atttcccact cctcttcaga gtacagaaga ttaagtgaga
     2521 ccttcgtttg tgcggatccc ccacacacca tagcttcaaa atgtttctac tcctttttta
     2581 ctcttccaga ttttctcgga ctccgcgcat cgccgtacca cttcaaaaca cccaagcaca
     2641 gcatactaaa tttcccctct ttcttcctct agggtgtcgt taattacccg tactaaaggt
     2701 ttggaaaaga aaaaagagac cgcctcgttt ctttttcttc gtcgaaaaag gcaataaaaa
     2761 tttttatcac gtttcttttt cttgaaaatt tttttttttg atttttttct ctttcgacga
     2821 cctcccattg atatttaagt taataaacgg tcttcaattt ctcaagtttc agtttcattt
     2881 ttcttgttct attacaactt tttttacttc ttgctcatta gaaagaaagc atagcaatct
     2941 aatctaaggg cggtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     3001 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     3061 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     3121 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     3181 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     3241 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     3301 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     3361 actgcgtgca cttcgtggcc gaggagcagg actgacacgt ccgacgcggc ccgacgggtc
     3421 cgaggcctcg gagatccgtc ccccttttcc tttgtcgata tcatgtaatt agttatgtca
     3481 cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa
     3541 cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt
     3601 atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg
     3661 aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcaa gctggagacc
     3721 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3781 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3841 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3901 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3961 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4021 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4081 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4141 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4201 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4261 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4321 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4441 gtcatgagat cagatctctg ctactctggt cccaagtgaa ccaccttttg gaccctattg
     4501 accggacctt aacttgccaa
//
