LOCUS       sequence-438338-        8173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ScChd1-aa142-1274 chromatin remodeler with ChEx domain deleted.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8173)
  AUTHORS   Nodelman IM, Das S, Faustino AM, Fried SD, Bowman GD, Armache JP
  TITLE     Nucleosome recognition and DNA distortion by the Chd1 remodeler in a
            nucleotide-free state.
  JOURNAL   Nat Struct Mol Biol. 2022 Feb;29(2):121-129. doi: 
            10.1038/s41594-021-00719-x. Epub 2022 Feb 16.
  PUBMED    35173352
REFERENCE   2  (bases 1 to 8173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-021-00719-x"; journalName: "Nat Struct Mol Biol"; 
            date: "2022-02"; volume: "29"; issue: "2"; pages: "121-129"
COMMENT     Sequence Label: pDEST17-ScChd1[∆ChEx]
FEATURES             Location/Qualifiers
     source          1..8173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..40
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             71..93
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             113..130
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    140..164
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             185..208
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    complement(3642..3666)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3719..3737)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3733..3780
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(4146..4151,4152..4168,4169..4174))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(4201..4219)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4287..4391
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4392..4460,4461..5252)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4610..4629)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5423..6011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5912..5931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6165..6182
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6197..6336
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6262..6281)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6422..6444
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6438..6629)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     8091..8110
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agatctcgat cccgcgaaat taatacgact cactataggg agaccacaac ggtttccctc
       61 tagaaataat tttgtttaac tttaagaagg agatatacat atgtcgtact accatcacca
      121 tcaccatcac ctcgaatcaa caagtttgta caaaaaagca ggctccgcgg ccgccccctt
      181 caccctggaa gttctgttcc aggggcccag cgatgatgac ctcttagaat ctgaagatga
      241 ctatggttcc gaggaggctt tatcagagga aaatgtgcat gaagcatctg ccaatcctca
      301 accagaggac ttccatggta ttgatattgt tatcaatcac agactaaaga catctttgga
      361 agaaggaaag gtcttggaaa agactgttcc tgacctcaat aactgcaagg aaaattatga
      421 attcttaatc aaatggacag atgaatctca tctccacaat acctgggaaa cttatgaatc
      481 cattggccaa gtccgtggat tgaagaggtt ggacaactat tgtaagcaat tcatcatcga
      541 agatcaacaa gtaagattag atccatacgt taccgccgaa gatatagaga tcatggacat
      601 ggaacgtgaa cgtaggcttg atgaatttga ggagttccac gtgccagaaa ggattattga
      661 tagccaacgt gcttcgttgg aagacggcac ctcacaatta cagtatttag ttaaatggcg
      721 tcgtctaaat tacgacgagg ctacttggga gaatgccaca gatatcgtga aattggcacc
      781 tgaacaagtg aaacattttc aaaacagaga aaactctaag atcctcccac aatattccag
      841 taattatact agtcagagac cgcgttttga gaagttaagc gtgcaacctc cgttcattaa
      901 aggtggggaa ttaagagatt ttcaactaac tggtattaat tggatggcat ttttgtggtc
      961 caaaggtgat aatggtatac tggcagatga gatgggcctg ggaaaaacgg tccagactgt
     1021 cgcctttatc agttggctga tatttgctcg tagacaaaac ggacctcaca tcattgtcgt
     1081 tcctttatcg acaatgcctg cctggttgga tacttttgag aaatgggcgc ctgatttgaa
     1141 ttgtatatgc tatatgggca accaaaaatc aagagatacc attcgagaat atgaatttta
     1201 caccaatcca agggcaaaag ggaaaaaaac aatgaaattt aatgttttat taacaacata
     1261 cgagtacatc ttaaaggatc gtgctgaatt aggaagtata aaatggcaat ttatggccgt
     1321 tgacgaagct catagactaa aaaatgctga atcatccctt tatgaatcat taaacagttt
     1381 caaggtcgcc aaccgtatgt taatcacagg cacacctctt cagaataata ttaaagagtt
     1441 agctgcgttg gttaatttcc taatgcccgg aaggtttacg attgatcagg agattgattt
     1501 tgaaaaccaa gatgaagagc aagaagaata tattcatgat ttacaccgaa gaatacagcc
     1561 ttttattctt cgtcggttga agaaagacgt agaaaaatca cttccatcaa agacagagcg
     1621 tattttaaga gttgaattgt ccgacgtaca gactgagtac tataaaaata ttctgactaa
     1681 aaactactct gctttaactg ctggagctaa agggggtcat ttctctttac tgaatattat
     1741 gaacgagttg aaaaaggcat cgaaccatcc atatctcttc gataatgctg aagagcgcgt
     1801 cttacagaaa tttggggatg gtaaaatgac tcgagaaaac gtactaagag gtttgatcat
     1861 gtcttcgggt aagatggttc ttttagacca attattgacc agattgaaga aagatgggca
     1921 ccgcgtgttg attttttcac aaatggtcag aatgcttgac attttaggcg actatttatc
     1981 cattaaaggt attaacttcc aaaggttaga tggtacagta ccatctgctc aaagaaggat
     2041 atctattgat catttcaact ctccggattc aaacgacttc gtatttttac tttctactcg
     2101 tgctggtggt ttgggtatca acttaatgac cgccgatacc gttgtgattt ttgattccga
     2161 ttggaatccg caagccgatt tacaagcaat ggctagagct catcgtattg gccaaaaaaa
     2221 tcacgttatg gtgtataggt tggtttcaaa agacacagta gaggaagagg tattagaaag
     2281 agcacggaag aaaatgattc tggaatatgc tattatttct cttggagtga cagatggtaa
     2341 caaatacact aagaagaatg aaccaaatgc cggtgaatta tcggcaatcc tgaagttcgg
     2401 tgcaggcaat atgttcactg ccacagacaa ccagaaaaaa ttggaggatc ttaacttgga
     2461 tgatgttttg aatcatgcag aggatcacgt tactactcca gacttaggag agtcgcatct
     2521 tggcggtgaa gagtttttga agcaattcga agtgactgat tataaagctg atatagattg
     2581 ggatgatatc attccagaag aagaactaaa aaaactccaa gatgaagagc agaaacgcaa
     2641 ggatgaagaa tatgttaaag aacaacttga aatgatgaat agaagggata acgccctaaa
     2701 aaaaataaaa aacagtgtta atggtgatgg gaccgccgca aactcagatt ctgacgacga
     2761 tagtacttcc agatcttcta gaagaagagc aagagctaat gacatggact ctattggtga
     2821 atcggaggtg agagctttgt ataaagctat tttaaaattt ggcaacctaa aggagatttt
     2881 ggacgagttg attgcagatg gaaccctgcc ggtcaaatca tttgaaaaat acggtgaaac
     2941 ttacgatgaa atgatggaag cagctaaaga ctgtgtacac gaggaagaga aaaatagaaa
     3001 agaaatcttg gagaaacttg agaagcatgc taccgcctat agagcaaagc taaaaagcgg
     3061 tgaaataaaa gcagagaacc aaccaaagga taatccgttg actagattat ctttaaaaaa
     3121 aagagaaaag aaagccgtcc ttttcaactt taaaggtgtt aaatctttaa acgccgaatc
     3181 tttactaagt agagtggagg atttaaagta cttgaagaac ttgataaatt caaactacaa
     3241 agatgatcca ttaaagttta gcctaggcaa caacacacct aagcctgtac agaattggtc
     3301 atctaattgg acgaaagagg aagatgagaa gctattgatt ggtgttttca aatatggata
     3361 tggttcctgg acacaaataa gagacgatcc atttctaggc attactgata aaatattctt
     3421 gaatgaagtt cacaatccgg tagcaaaaaa gtcggcaagc tcctctgata caacaccaac
     3481 accctcaaag aaaggaaagg gaataacggg ttcttcaaaa aaagtacctg gtgccattca
     3541 cttgggcaga agagttgatt atctattatc cttcttaaga ggaggcctaa atacaaagag
     3601 tcccagttga aagcttaata ataataaggg tgggcgcgcc gacccagctt tcttgtacaa
     3661 agtggttgat tcgaggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
     3721 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     3781 ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta
     3841 gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga
     3901 cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac
     3961 gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac
     4021 cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag
     4081 cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact
     4141 accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg
     4201 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4261 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4321 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4381 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4441 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4501 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4561 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4621 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4681 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4741 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4801 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4861 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4921 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4981 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5041 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5101 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5161 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5221 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5281 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5341 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5401 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5461 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5521 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     5581 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5641 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5701 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5761 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5821 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5881 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5941 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6001 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6061 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6121 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6181 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     6241 accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     6301 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     6361 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     6421 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     6481 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
     6541 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
     6601 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc
     6661 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
     6721 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
     6781 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
     6841 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
     6901 gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct
     6961 caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca
     7021 ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg
     7081 atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg
     7141 gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc
     7201 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg
     7261 cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga
     7321 caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg
     7381 cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga
     7441 gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
     7501 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
     7561 ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa
     7621 tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg
     7681 cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc
     7741 ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa
     7801 agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga
     7861 ctgggttgaa ggctctcaag ggcatcggtc gatcgacgct ctcccttatg cgactcctgc
     7921 attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt
     7981 gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
     8041 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
     8101 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
     8161 gcgtagagga tcg
//
