LOCUS       sequence-43834-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Park HS, Hohn MJ, Umehara T, Guo LT, Osborne EM, Benner J, Noren CJ,
            Rinehart J, Soll D
  TITLE     Expanding the genetic code of Escherichia coli with phosphoserine.
  JOURNAL   Science. 2011 Aug 26;333(6046):1151-4.
  PUBMED    21868676
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-08-26"; volume: "333(6046)"; pages: "1151-4"
COMMENT     Sequence Label: pMAL-EFSep
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     131..153
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     145..162
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     146..162
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     182..200
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(253..270)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agctgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
       61 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
      121 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct tgcctgcaga
      181 tgctagttat tgctcagcgg tggcagcagc caactcagct tcctttcggg ctttgttagc
      241 agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct tgtcgacgga
      301 gctcgaattc ggatccctca gaacttttgc tacaacgccc gcgccaacgg tacggccgcc
      361 ttcacggatt gcgaaacgca gaccgtcgtc catcgcgatc gggtggatca gggtaacaac
      421 cattttgatg ttgtcgcccg gcattaacat ctctacgcct tccggcagtt cgatggtacc
      481 agtcacgtca gtagtacgga agtagaactg cggacggtag cctttgaaga acggagtatg
      541 acggccgcct tcatctttgg acagaatgta cacttcagat tcgaacttgg tgtgcggctt
      601 gatggtgccc ggcttagcca gtacctgacc acgttcgatt tcttcacgtt tgataccacg
      661 cagcagaaca cctacccact caccagcacg gccttcgtcc agcagtttgc ggaacatttc
      721 aacgccagta caggtagact tctgagtctc tttgatacca acgatttcaa cttcttcacc
      781 aactttgatg ataccgcgtt ctacacgacc cgaaacaacg gtaccacgac cggagatgga
      841 gtataccccg ttgatcggca gcaggaacgg cttgtcaatc gcacgctctg gttccggaat
      901 gtaagaatcc aggaagcc
//
