LOCUS       sequence-438347-        4985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Xenopus laevis histone H3 with Nterminal tail swapped for ChEx 
            domain of ScChd1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Nodelman IM, Das S, Faustino AM, Fried SD, Bowman GD, Armache JP
  TITLE     Nucleosome recognition and DNA distortion by the Chd1 remodeler in a
            nucleotide-free state.
  JOURNAL   Nat Struct Mol Biol. 2022 Feb;29(2):121-129. doi: 
            10.1038/s41594-021-00719-x. Epub 2022 Feb 16.
  PUBMED    35173352
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-021-00719-x"; journalName: "Nat Struct Mol Biol"; 
            date: "2022-02"; volume: "29"; issue: "2"; pages: "121-129"
COMMENT     Sequence Label: pET3a-XlH3[ChEx]
FEATURES             Location/Qualifiers
     source          1..4985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(10..15,16..32,33..38)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(404..451)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     447..465
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     RBS             907..929
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(960..979)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(961..979)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1063..1082)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             2540..2731
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(2725..2747)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2833..2972
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2888..2907
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2987..3004)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3158..3746)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3238..3257)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3917..4708,4709..4777))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4540..4559
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4778..4882)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4950..4968
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
      361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
      421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
      481 cagcttcctt tcgggctttg ttagcagccg gatccttaag ccctctcgcc tcggattctg
      541 cgggccagct ggatgtcctt gggcatgatg gtgaccctct tggcgtggat ggcgcacagg
      601 ttggtgtcct caaagagagc gaccagataa gcctcgctgg cctcctgcag agccataacg
      661 gccgagctct ggaagcgcag gtcggtcttg aagtcctgag cgatctcccg gaccaggcgc
      721 tggaaaggca gtttgcggat gagcagctcg gtggatttct ggtagcggcg gatctcgcgg
      781 agagcgactg tgccgggccg accgctgccg aggtcatcat cgctataatc gatgttataa
      841 tttaccgttt tgttttgacg attagaaaac cttgttggta ttttcactgt agattgcata
      901 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg ttgtggtctc
      961 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac gccggacgca
     1021 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     1081 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     1141 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     1201 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     1261 cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     1321 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     1381 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga
     1441 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag
     1501 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca
     1561 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct
     1621 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
     1681 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta
     1741 ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc gcctcggcga
     1801 gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg
     1861 cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct
     1921 cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa
     1981 tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac
     2041 gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc
     2101 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat
     2161 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg
     2221 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac
     2281 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
     2341 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca
     2401 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac
     2461 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc
     2521 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt
     2581 tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca
     2641 ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc
     2701 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     2761 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     2821 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     2881 aactatgcgg catcagagca gattgtactg agagtgcacc attgcggtgt gaaataccgc
     2941 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     3001 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3061 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3121 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3181 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3241 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3301 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3361 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3421 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3481 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3541 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     3601 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3661 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3721 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3781 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3841 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3901 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3961 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4021 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4081 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4141 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4201 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     4261 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4321 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4381 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4441 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4501 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     4561 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4621 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4681 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4741 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4801 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4861 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     4921 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     4981 aagaa
//
