LOCUS       sequence-438388-        8175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Split intein version of Trojan-Gal4 Expression Module in Phase 2. 
            Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be 
            converted by cassette exchange into other Trojan exons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Diao F, Vasudevan D, Heckscher ES, White BH
  TITLE     Hox gene-specific cellular targeting using split intein Trojan 
            exons.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Apr 23;121(17):e2317083121. doi: 
            10.1073/pnas.2317083121. Epub 2024 Apr 11.
  PUBMED    38602904
REFERENCE   2  (bases 1 to 8175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-04-23"; volume: "121(17):e2317083121. 
            doi"; pages: " 10.1073/pnas.2317083121. Epub 2024 Apr 11"
COMMENT     Sequence Label: psiT-GEM(2)
FEATURES             Location/Qualifiers
     source          1..8175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..19
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3..19
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..171
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     CDS             588..641
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             642..3284
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             3285..3338
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    3576..3609
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          4596..4661
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4791..4811
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5083..5217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5120..5139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5174..5193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    5225..5258
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    5259..5358
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     primer_bind     complement(5389..5409)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5389..5407)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5428..5444)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5428..5444)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5441..5463)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5452..5468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5476..5482,5483..5500,5501..5506))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5521..5542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5659..5676)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5830..6418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5910..5929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6589..7380,7381..7449))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7212..7231
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7450..7554)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7581..8036
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7668..7687)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7878..7899
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     join(8163..8175,1..10)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgacc caccggtgca
       61 tgcgcggccg ccccaggtca gaagcggttt tcgggagtag tgccccaact ggggtaacct
      121 ttgagttctc tcagttgggg gcgtagggtc gccgacatga cacaaggggt ttctagaagt
      181 cgatccaaca tggcgacttg tcccatcccc ggcatgttta aatatactaa ttattcttga
      241 actaatttta atcaaccgat ttatctctct tccgcaggga atattgcctg tcctacgaca
      301 ccgagatcct gaccgtggag tacggcttcc tgcccatcgg caagatcgtg gaggagcgca
      361 tcgagtgcac cgtgtacacc gtggacaaga acggcttcgt gtacacccag cccatcgccc
      421 agtggcacaa ccgcggcgag caggaggtgt tcgagtactg cctggaggac ggctccatca
      481 tccgcgccac caaggaccac aagttcatga ccaccgacgg ccagatgctg cccatcgacg
      541 agatcttcga gcgcggcctg gacctgaagc aggtggacgg cctgcccgag ggccgcggca
      601 gcctgctgac ctgcggcgat gtggaggaga accccgggcc catgaagcta ctgtcttcta
      661 tcgaacaagc ctgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      721 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      781 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      841 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
      901 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
      961 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1021 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1081 cgattgactc ggcagctcat catgataact ccacaattcc gttggatttt atgcccaggg
     1141 atgctcttca tggatttgat tggtctgaag aggatgacat gtcggatggc ttgcccttcc
     1201 tgaaaacgga ccccaacaat aatgggttct ttggcgacgg ttctctctta tgtattcttc
     1261 gatctattgg ctttaaaccg gaaaattaca cgaactctaa cgttaacagg ctcccgacca
     1321 tgattacgga tagatacacg ttggcttcta gatccacaac atcccgttta cttcaaagtt
     1381 atctcaataa ttttcacccc tactgcccta tcgtgcactc accgacgcta atgatgttgt
     1441 ataataacca gattgaaatc gcgtcgaagg atcaatggca aatccttttt aactgcatat
     1501 tagccattgg agcctggtgt atagaggggg aatctactga tatagatgtt ttttactatc
     1561 aaaatgctaa atctcatttg acgagcaagg tcttcgagtc aggttccata attttggtga
     1621 cagccctaca tcttctgtcg cgatatacac agtggaggca gaaaacaaat actagctata
     1681 attttcacag cttttccata agaatggcca tatcattggg cttgaatagg gacctcccct
     1741 cgtccttcag tgatagcagc attctggaac aaagacgccg aatttggtgg tctgtctact
     1801 cttgggagat ccaattgtcc ctgctttatg gtcgatccat ccagctttct cagaatacaa
     1861 tctccttccc ttcttctgtc gacgatgtgc agcgtaccac aacaggtccc accatatatc
     1921 atggcatcat tgaaacagca aggctcttac aagttttcac aaaaatctat gaactagaca
     1981 aaacagtaac tgcagaaaaa agtcctatat gtgcaaaaaa atgcttgatg atttgtaatg
     2041 agattgagga ggtttcgaga caggcaccaa agtttttaca aatggatatt tccaccaccg
     2101 ctctaaccaa tttgttgaag gaacaccctt ggctatcctt tacaagattc gaactgaagt
     2161 ggaaacagtt gtctcttatc atttatgtat taagagattt tttcactaat tttacccaga
     2221 aaaagtcaca actagaacag gatcaaaatg atcatcaaag ttatgaagtt aaacgatgct
     2281 ccatcatgtt aagcgatgca gcacaaagaa ctgttatgtc tgtaagtagc tatatggaca
     2341 atcataatgt caccccatat tttgcctgga attgttctta ttacttgttc aatgcagtcc
     2401 tagtacccat aaagactcta ctctcaaact caaaatcgaa tgctgagaat aacgagaccg
     2461 cacaattatt acaacaaatt aacactgttc tgatgctatt aaaaaaactg gccactttta
     2521 aaatccagac ttgtgaaaaa tacattcaag tactggaaga ggtatgtgcg ccgtttctgt
     2581 tatcacagtg tgcaatccca ttaccgcata tcagttataa caatagtaat ggtagcgcca
     2641 ttaaaaatat cgtcggttct gcaactatcg cccaataccc tactcttccg gaggaaaatg
     2701 tcaacaatat cagtgttaaa tatgtttctc ctggctcagt agggccttca cctgtgccat
     2761 tgaaatcagg agcaagtttc agtgatctag tcaagctgtt atctaaccgt ccaccctctc
     2821 gtaactctcc agtgacaata ccaagaagca caccttcgca tcgctcagtc acgccttttc
     2881 tagggcaaca gcaacagctg caatcattag tgccactgac cccgtctgct ttgtttggtg
     2941 gcgccaattt taatcaaagt gggaatatcg ctgatagctc attgtccttc actttcacta
     3001 acagtagcaa cggtccgaac ctcataacaa ctcaaacaaa ttctcaagcg ctttcacaac
     3061 caattgcctc ctctaacgtt catgataact tcatgaataa tgaaatcacg gctagtaaaa
     3121 ttgatgatgg taataattca aaaccactgt cacctggttg gacggaccaa actgcgtata
     3181 acgcgtttgg aatcactaca gggatgttta ataccactac aatggatgat gtatataact
     3241 atctattcga tgatgaagat accccaccaa acccaaaaaa agaggagggc cgcggcagcc
     3301 tgctgacctg cggcgatgtg gaggagaacc ccgggcccgt gaagatcatc tcccgcaagt
     3361 ccctgggcac ccagaacgtg tacgacatcg gcgtggagaa ggaccacaac ttcctgctga
     3421 agaacggcct ggtggcctcc aactgcttca acaggtaagt tattgaacaa tggcatcaaa
     3481 tgccttcatc atcactaccc tttagccctt aagaccccac aatgacctta cccactcaga
     3541 gaaaaaagta aatatgaaag cccatttgaa cttctgaagt tcctattctc tagaaagtat
     3601 aggaacttct cctaggggat ctaattcaat tagagactaa ttcaattaga gctaattcaa
     3661 ttaggatcca agcttatcga tttcgaaccc tcgaccgccg gagtataaat agaggcgctt
     3721 cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct aagcgaaagc
     3781 taagcaaata aacaagcgca gctgaacaag ctaaacaatc ggactagagc cggtcgccac
     3841 catgaggtct tccaagaatg ttatcaagga gttcatgagg tttaaggttc gcatggaagg
     3901 aacggtcaat gggcacgagt ttgaaataga aggcgaagga gaggggaggc catacgaagg
     3961 ccacaatacc gtaaagctta aggtaaccaa ggggggacct ttgccatttg cttgggatat
     4021 tttgtcacca caatttcagt atggaagcaa ggtatatgtc aagcaccctg ccgacatacc
     4081 agactataaa aagctgtcat ttcctgaagg atttaaatgg gaaagggtca tgaactttga
     4141 agacggtggc gtcgttactg taacccagga ttccagtttg caggatggct gtttcatcta
     4201 caaggtcaag ttcattggcg tgaactttcc ttccgatgga cctgttatgc aaaagaagac
     4261 aatgggctgg gaagccagca ctgagcgttt gtatcctcgt gatggcgtgt tgaaaggaga
     4321 gattcataag gctctgaagc tgaaagacgg tggtcattac ctagttgagt tcaaaagtat
     4381 ttacatggca aagaagcctg tgcagctacc agggtactac tatgttgact ccaaactgga
     4441 tataacaagc cacaacgaag actatacaat cgttgagcag tatgaaagaa ccgagggacg
     4501 ccaccatctg ttcctttagg atctttgtga aggaacctta cttctgtggt gtgacataat
     4561 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat
     4621 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg
     4681 atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa
     4741 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga
     4801 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg
     4861 ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg
     4921 cactgctata caagaaaatt atggaaaaat atttgatgta tagtgccttg actagagatc
     4981 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     5041 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     5101 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5161 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgct
     5221 agcagaagtt cctattctct agaaagtata ggaacttcaa ccccttgtgt catgtcggcg
     5281 accctacgcc cccaactgag agaactcaaa ggttacccca gttggggcac tactcccgaa
     5341 aaccgcttct gacctggggg cgcgccggta ccactagtgg ggaggttccc tttagtgagg
     5401 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5461 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5521 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5581 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5641 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6241 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6481 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6541 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6601 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6661 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6721 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6781 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6841 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6901 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6961 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7021 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7081 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7141 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7201 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7261 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7321 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7381 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7441 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7501 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7561 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     7621 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7681 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7741 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7801 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7861 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7921 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7981 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttgcca
     8041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     8101 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     8161 ttcccagtca cgacg
//
