LOCUS       sequence-438436-        9705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway backbone for C-terminal dTag cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Pulice JL, Meyerson M
  TITLE     Dosage amplification dictates oncogenic regulation by the NKX2-1 
            lineage factor in lung adenocarcinoma.
  JOURNAL   bioRxiv [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 
            10.1101/2023.10.26.563996.
  PUBMED    37994369
REFERENCE   2  (bases 1 to 9705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 
            10.1101/2023.10.26.563996."
COMMENT     Sequence Label: pLX304_CdTag-HA_EmptyVector
FEATURES             Location/Qualifiers
     source          1..9705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1710..2210
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2129..2147
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2222..2617
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2677..2794
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2911..3214
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3215..3418
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3369..3389
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3441..3565
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3590..3595,3596..3613,3614..3620)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3674..4333
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3740..3759)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4675..4980
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4859..4878
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5021..5145)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5153..5473
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             5480..5506
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5480..5506
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5510..5536
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5558..6146
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5611..5631)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6029..6040)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6218..6451
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6529..6650
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6566..6585)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6620..6639
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6690..6825
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6845..6864)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6846..6864)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6874..6891)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6874..6890)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6883..6905)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7032..7487
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7119..7138)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7329..7350
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7513..7617
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7618..7686,7687..8478)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7836..7855)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8649..9237
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9138..9157
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9391..9408
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9525..9546
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9561..9566,9567..9584,9585..9591)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9599..9615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9604..9626
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9623..9639
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9623..9639
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9658..9678
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9660..9678
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccagggtga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccct tgcgcctttt ccaaggcagc cctgggtttg
     1741 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     1801 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     1861 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     1921 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     1981 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     2041 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     2101 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     2161 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggtaccac
     2221 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     2281 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     2341 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     2401 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     2461 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     2521 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     2581 tcgtgaattg ctgccctctg gttatgtgtg ggagggcctg cagctgcagt agtaagaatt
     2641 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     2701 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2761 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     2821 tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa ttacggggtc
     2881 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2941 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     3001 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     3061 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     3121 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     3181 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     3241 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     3301 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     3361 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
     3421 ctggctaact gtcgggatca acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg
     3481 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
     3541 aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta
     3601 tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc
     3661 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     3721 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     3781 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     3841 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc
     3901 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     3961 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     4021 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     4081 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     4141 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     4201 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
     4261 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     4321 gggcggggcg taaagatctg gatccggctt actaaaagcc agataacagt atgcgtattt
     4381 gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa
     4441 aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg
     4501 ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag
     4561 cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg
     4621 cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag
     4681 tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt
     4741 gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg
     4801 tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga aagctggcgc
     4861 atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat
     4921 ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa
     4981 atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg
     5041 tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt
     5101 tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggttaccg gtggagtgca
     5161 ggtggaaacc atctccccag gagacgggcg caccttcccc aagcgcggcc agacctgcgt
     5221 ggtgcactac accgggatgc ttgaagatgg aaagaaagtt gattcctccc gggacagaaa
     5281 caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg aagaaggggt
     5341 tgcccagatg agtgtgggtc agagagccaa actgactata tctccagatt atgcctatgg
     5401 tgccactggg cacccaggca tcatcccacc acatgccact ctcgtcttcg atgtggagct
     5461 tctaaaactg gaaggcggct acccctacga cgtgcccgac tacgccggct atccgtatga
     5521 tgtcccggac tatgcaggct aaacgcgtta agtcgacaat caacctctgg attacaaaat
     5581 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5641 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5701 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5761 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5821 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5881 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5941 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     6001 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6061 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6121 gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct
     6181 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     6241 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     6301 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6361 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6421 cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt
     6481 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     6541 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6601 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6661 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6721 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     6781 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     6841 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     6901 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     6961 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     7021 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     7081 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     7141 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     7201 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     7261 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     7321 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     7381 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     7441 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     7501 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7561 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7621 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7681 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7741 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7801 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7861 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7921 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7981 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     8041 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8101 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8161 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     8221 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     8281 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     8341 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8401 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8461 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8521 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8581 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8641 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8701 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8761 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8821 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8881 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8941 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9001 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9061 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9121 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9181 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     9241 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     9301 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     9361 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     9421 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     9481 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     9541 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     9601 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     9661 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaa
//
