LOCUS       sequence-438437-        9901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway backbone for C-terminal dTag cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Pulice JL, Meyerson M
  TITLE     Dosage amplification dictates oncogenic regulation by the NKX2-1 
            lineage factor in lung adenocarcinoma.
  JOURNAL   bioRxiv [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 
            10.1101/2023.10.26.563996.
  PUBMED    37994369
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Oct 27:2023.10.26.563996. doi: 
            10.1101/2023.10.26.563996."
COMMENT     Sequence Label: pLX302_CdTag-HA_EmptyVector
FEATURES             Location/Qualifiers
     source          1..9901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1710..2210
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2129..2147
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2232..2831
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2232..2251)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2728..2748
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2873..2990
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3107..3410
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3411..3614
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3565..3585
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3637..3761
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3786..3791,3792..3809,3810..3816)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3870..4529
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3936..3955)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4871..5176
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5055..5074
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5217..5341)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5349..5669
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             5676..5702
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5676..5702
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5706..5732
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    5754..6342
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5807..5827)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6225..6236)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6414..6647
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6725..6846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6762..6781)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6816..6835
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6886..7021
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7041..7060)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7042..7060)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7070..7087)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7070..7086)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7079..7101)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7228..7683
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7315..7334)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7525..7546
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7709..7813
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7814..7882,7883..8674)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8032..8051)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8845..9433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9334..9353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9587..9604
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9721..9742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9757..9762,9763..9780,9781..9787)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9795..9811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9800..9822
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9819..9835
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9819..9835
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9854..9874
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9856..9874
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccct tgcgcctttt ccaaggcagc cctgggtttg
     1741 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     1801 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     1861 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     1921 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     1981 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     2041 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     2101 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     2161 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggtaccac
     2221 cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     2281 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     2341 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     2401 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga
     2461 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     2521 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     2581 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     2641 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     2701 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     2761 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     2821 ccggtgcctg agaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
     2881 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2941 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     3001 acagggacag cagagatcca ctttggcgcc ggctcgaggg ggcccggggt tattaatagt
     3061 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     3121 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     3181 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     3241 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     3301 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     3361 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     3421 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     3481 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     3541 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     3601 atataagcag agctctctgg ctaactgtcg ggatcaacaa gtttgtacaa aaaagctgaa
     3661 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
     3721 actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc
     3781 caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt
     3841 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     3901 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     3961 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     4021 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     4081 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     4141 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     4201 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     4261 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     4321 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     4381 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     4441 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     4501 actgcgatga gtggcagggc ggggcgtaaa gatctggatc cggcttacta aaagccagat
     4561 aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata
     4621 cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag
     4681 cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca
     4741 accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa
     4801 gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg
     4861 ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt
     4921 tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc
     4981 cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg
     5041 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg
     5101 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
     5161 gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata
     5221 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
     5281 ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg
     5341 ttaccggtgg agtgcaggtg gaaaccatct ccccaggaga cgggcgcacc ttccccaagc
     5401 gcggccagac ctgcgtggtg cactacaccg ggatgcttga agatggaaag aaagttgatt
     5461 cctcccggga cagaaacaag ccctttaagt ttatgctagg caagcaggag gtgatccgag
     5521 gctgggaaga aggggttgcc cagatgagtg tgggtcagag agccaaactg actatatctc
     5581 cagattatgc ctatggtgcc actgggcacc caggcatcat cccaccacat gccactctcg
     5641 tcttcgatgt ggagcttcta aaactggaag gcggctaccc ctacgacgtg cccgactacg
     5701 ccggctatcc gtatgatgtc ccggactatg caggctaaac gcgttaagtc gacaatcaac
     5761 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     5821 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     5881 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     5941 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6001 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6061 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6121 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     6181 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6241 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     6301 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat
     6361 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     6421 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     6481 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6541 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6601 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag
     6661 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     6721 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6781 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6841 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     6901 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6961 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7021 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     7081 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     7141 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     7201 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     7261 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     7321 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     7381 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7441 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     7501 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     7561 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7621 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     7681 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7741 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7801 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7861 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7921 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7981 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8041 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8101 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8161 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8221 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8281 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8341 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8401 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8461 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8521 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8581 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8641 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8701 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8761 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagacca
     8821 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8881 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8941 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9001 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9061 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     9121 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9181 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9241 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     9301 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     9361 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9421 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     9481 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     9541 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     9601 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     9661 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     9721 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     9781 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     9841 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     9901 a
//
