LOCUS       sequence-438455-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 spike S371F for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 S371F (TTC)
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(230..298,299..1090)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(448..467)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      1103..1239
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    1391..1462
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(1467..1513)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    1531..1579
                     /note="synthetic polyadenylation signal"
     enhancer        1660..1963
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1965..2167
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2118..2138
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          2301..2776
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     misc_feature    6673..7261
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6726..6746)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7144..7155)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7314..7435
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7325..7344)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7379..7398
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      7550..7596
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      7626..7762
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    7807..7878
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        7923..8119
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7970..8105
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8133..8927
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8187..8206)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8797..8816
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8938..9162
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(9358..9946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9438..9457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctctcagtg cgagtctcga cgatccatat cgttgcttgg cagtcagcca gtcggaatcc
       61 agcttgggac ccaggaagtc caatcgtcag atattgtact caagcctggt cacggcagcg
      121 taccgatctg tttaaaccta gatattgata gtctgatcgg tcaacgtata atcgagtcct
      181 agcttttgca aacatctatc aagagacagg atcagcagga ggctttcgca tgagtattca
      241 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
      301 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcgc gagtgggtta
      361 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgctt
      421 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
      481 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtattc
      541 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
      601 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgattg gaggaccgaa
      661 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
      721 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
      781 ggcaacaacc ttgcgtaaac tattaactgg cgaactactt actctagctt cccggcaaca
      841 gttgatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
      901 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
      961 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     1021 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     1081 gcattggtaa ccgattctag gtgcattggc gcagaaaaaa atgcctgatg cgacgctgcg
     1141 cgtcttatac tcccacatat gccagattca gcaacggata cggcttcccc aacttgccca
     1201 cttccatacg tgtcctcctt accagaaatt tatccttaag atcccgaatc gtttaaactc
     1261 gactctggct ctatcgaatc tccgtcgttt cgagcttacg cgaacagccg tggcgctcat
     1321 ttgctcgtcg ggcatcgaat ctcgtcagct atcgtcagct tacctttttg gcagcgatcg
     1381 cggctcccga catcttggac cattagctcc acaggtatct tcttccctct agtggtcata
     1441 acagcagctt cagctacctc tcaattcaaa aaacccctca agacccgttt agaggcccca
     1501 aggggttatg ctatcaatcg ttgcgttaca cacacaaaaa accaacacac atccatcttc
     1561 gatggatagc gattttatta tctaactgct gatcgagtgt agccagatct agtaatcaat
     1621 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     1681 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     1741 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     1801 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     1861 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     1921 tacttggcag tacatctacg tattagtcat cgctattacc atgctgatgc ggttttggca
     1981 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2041 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2101 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2161 cagagctggt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2221 ccatagaaga caccgggacc gatccagcct cccctcgaag cttacatgtg gtaccgagct
     2281 cggatcctga gaacttcagg gtgagtctat gggacccttg atgttttctt tccccttctt
     2341 ttctatggtt aagttcatgt cataggaagg ggagaagtaa cagggtacac atattgacca
     2401 aatcagggta attttgcatt tgtaatttta aaaaatgctt tcttctttta atatactttt
     2461 ttgtttatct tatttctaat actttcccta atctctttct ttcagggcaa taatgataca
     2521 atgtatcatg cctctttgca ccattctaaa gaataacagt gataatttct gggttaaggc
     2581 aatagcaata tttctgcata taaatatttc tgcatataaa ttgtaactga tgtaagaggt
     2641 ttcatattgc taatagcagc tacaatccag ctaccattct gcttttattt tatggttggg
     2701 ataaggctgg attattctga gtccaagcta ggcccttttg ctaatcatgt tcatacctct
     2761 tatcttcctc ccacagctcc tgggcaacgt gctggtctgt gtgctggccc atcactttgg
     2821 caaaggcggc cgccatgttt gtgttcctcg ttctcctgcc cctcgtttct tcccaatgcg
     2881 tgaacctcac aactagaacg cagctcccac ccgcttacac gaactctttt actcgtggag
     2941 tatactatcc cgataaagtc tttaggtcca gtgtccttca ttctactcaa gaccttttcc
     3001 tgccattctt ctcaaatgtc acctggttcc atgctatcca cgtttctggc accaacggaa
     3061 ctaaacgatt tgataatccc gtcttgccat ttaacgatgg cgtatacttt gcctcaacgg
     3121 agaagtccaa cattatccgg ggatggatat ttggcaccac cctggactcc aagacacagt
     3181 ctctcctcat tgtgaacaac gccaccaatg tcgtcatcaa agtctgtgaa tttcaatttt
     3241 gtaacgatcc ctttctcggg gtttattatc ataagaataa caagtcttgg atggagtcag
     3301 agttccgcgt ttacagttcc gctaacaact gtacttttga gtacgtttcc caaccatttc
     3361 tcatggatct tgaagggaag caaggcaact tcaagaatct ccgcgaattt gtgtttaaga
     3421 atatagacgg ctacttcaaa atttatagca agcatacccc gataaatctg gttcgggatc
     3481 tccctcaagg cttctccgcg ttggaacccc tcgtggactt gccgattggc atcaacatca
     3541 cccggttcca aacccttctt gcccttcatc gaagctacct cacgcctggc gacagtagta
     3601 gtggttggac agcgggcgca gcagcctact atgtgggata tttgcaacca cgcacctttc
     3661 ttctcaaata taacgaaaac ggaacaatta ccgatgccgt agactgtgcc ctcgatcccc
     3721 tgtccgaaac taaatgcaca ctcaagtcct tcacagttga gaaaggcata taccaaacca
     3781 gtaacttccg cgtccaaccc accgaatcca ttgttcgatt ccctaacatc accaatttgt
     3841 gtcctttcgg tgaagtattt aatgccactc gatttgcatc agtttatgcc tggaatcgca
     3901 agcgaatatc aaattgtgtt gcagattata gtgtcctgta taatttcgca tcattcagca
     3961 cttttaagtg ttatggggtc agccctacga aattgaatga cttgtgtttt accaacgttt
     4021 acgcagattc tttcgtcata cgcggcgacg aagtccgaca gattgcaccg gggcaaaccg
     4081 gaaagatagc agactataat tataagttgc cggatgactt caccggctgc gttatagcat
     4141 ggaactccaa taatctcgat tcaaaagtcg gcggcaacta taattatctt taccgcctgt
     4201 ttcgcaagtc aaatctgaag ccctttgaac gtgacatctc caccgaaatt tatcaagctg
     4261 gcagcacccc atgtaatgga gtggaagggt ttaactgtta ttttcccctt caatcatacg
     4321 gatttcaacc aacaaatggg gtgggctatc aaccatatcg ggtggtagtc ttgagctttg
     4381 agctgctcca cgcaccggct acagtttgcg gaccgaagaa aagcactaac ctggtgaaga
     4441 ataaatgtgt gaacttcaac tttaatgggc tcaccggaac aggggtgctg acagaatcaa
     4501 acaagaaatt tctccctttt caacaatttg gtagagacat tgccgatacc acagatgccg
     4561 tccgcgatcc gcaaacactg gaaatcctcg atataacacc ctgtagcttc ggcggggtca
     4621 gcgtgataac tcccggaacg aacacatcca accaagtggc tgtcctgtat caagatgtca
     4681 actgtaccga agtccctgtg gcgatacacg ccgaccaact gacacccacc tggagagtct
     4741 actcaacagg ttccaatgtc tttcaaacta gagcgggttg tctcattggc gctgagcatg
     4801 tcaataattc atacgaatgt gatattccta ttggggcagg aatctgtgcg tcttatcaaa
     4861 ctcaaacgaa ctcaccacgg cgcgctcgct cagtagccag ccagtccata atcgcctata
     4921 ccatgagtct cggggctgag aactccgtgg cttattcaaa caattccatc gcaatcccaa
     4981 ctaactttac cattagcgtt accacggaaa tcttgcccgt cagcatgact aagacgagcg
     5041 tagactgtac gatgtatatc tgtggggatt ccaccgagtg tagcaacctc ttgcttcaat
     5101 acgggtcttt ctgtactcaa ctcaaccgag cattgaccgg aattgcggtc gaacaagaca
     5161 agaataccca agaggtgttc gcacaagtaa agcaaatata caaaacgccg ccaatcaaag
     5221 attttggcgg gtttaatttt agccaaatac tgccagaccc atctaagcct tcaaaacgca
     5281 gctttatcga ggatctgctg ttcaacaaag ttacgttggc tgacgcgggt ttcattaagc
     5341 agtatgggga ttgtttggga gacattgcgg cccgcgacct tatctgtgct caaaaattca
     5401 atgggttgac agttctgccc cctctgctca ctgatgagat gattgcacag tatacatcag
     5461 cattgttggc aggaaccatt acatccggct ggacttttgg cgcgggtgcg gcccttcaaa
     5521 tacctttcgc tatgcaaatg gcttacagat tcaatgggat tggtgtcacg caaaatgtat
     5581 tgtatgaaaa tcagaagctg atcgctaatc aatttaacag cgcgattggc aagatccaag
     5641 attcactgtc aagcacagcg tccgccctcg gcaaactcca agatgttgtg aatcaaaacg
     5701 cccaggccct gaataccctc gtgaagcaac tgagttctaa cttcggcgca atctccagcg
     5761 tcctcaacga catattgtcc agacttgata aggtggaagc cgaggtgcaa attgatcggc
     5821 tcatcacagg caggctccag tcactccaaa cttatgtgac acaacagttg atccgggctg
     5881 ctgagatacg agcaagcgcc aacctggccg ccactaaaat gtcagagtgc gtgctcgggc
     5941 agtccaaacg ggttgacttt tgtggcaagg gctatcactt gatgtccttc ccccaatccg
     6001 ccccacatgg agtggtcttt ctccacgtca cttatgtccc tgctcaagag aagaacttta
     6061 cgacagctcc agcaatctgt cacgatggca aagcgcactt tcccagggaa ggcgttttcg
     6121 tcagtaatgg cacgcattgg tttgtgaccc aaaggaattt ctatgaaccc caaatcataa
     6181 ccactgacaa tacatttgtc tcaggcaact gtgatgtggt cattgggatc gtgaacaaca
     6241 cggtatatga tcccctgcaa cccgagttgg atagcttcaa agaggaattg gataaatact
     6301 ttaagaatca tacttcaccc gatgttgatc tcggggacat atcaggaata aatgcaagtg
     6361 tcgtcaacat tcagaaagag attgatcgcc ttaatgaggt cgccaagaac ttgaatgaat
     6421 ctttgatcga cttgcaagaa ctgggcaaat acgaacaata tattaaatgg ccctggtaca
     6481 tttggttggg ctttatcgcc ggacttatag ctatcgttat ggtcacaatt atgttgtgct
     6541 gtatgactag ctgttgttcc tgcttgaaag gatgttgcag ttgcggatct tgttgtaaat
     6601 aaaggatcct ctagaagttg tctcctcctg cactgactga ctgatacaat cgatttctgg
     6661 atccgcaggc ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6721 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6781 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6841 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6901 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6961 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7021 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     7081 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7141 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7201 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7261 cctgtctagc ttgactgact gagatacagc gtaccttcag ctcacagaca tgataagata
     7321 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     7381 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     7441 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     7501 caagtaaaac ctctacaaat gtggtattgg cccatctcta tcggtatcgt agcataaccc
     7561 cttggggcct ctaaacgggt cttgaggggt tttttgtgcc cctcgggccg gattgctatc
     7621 taccggcatt ggcgcagaaa aaaatgcctg atgcgacgct gcgcgtctta tactcccaca
     7681 tatgccagat tcagcaacgg atacggcttc cccaacttgc ccacttccat acgtgtcctc
     7741 cttaccagaa atttatcctt aaggtcgtca gctatcctgc aggcgatctc tcgatttcga
     7801 tcaagacatt cctttaatgg tcttttctgg acaccactag gggtcagaag tagttcatca
     7861 aactttcttc cctccctaat ctcattggtt accttgggct atcgaaactt aattaagcga
     7921 tctgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7981 aactccgccc agttccgccc attctccgcc ccatcgctga ctaatttttt ttatttatgc
     8041 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     8101 aggcctaggc ttttgcaaag gaggtagcca acatgattga acaagatgga ttgcacgcag
     8161 gttctcccgc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     8221 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     8281 agaccgacct gtccggtgcc ctgaatgaac tccaggacga ggcagcgcgg ctatcgtggc
     8341 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     8401 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     8461 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     8521 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     8581 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     8641 tgttcgccag gctcaaggcg cggatgcccg acggcgagga tctcgtcgtg acccacggcg
     8701 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     8761 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     8821 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     8881 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctagtat gtaagccctg
     8941 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     9001 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     9061 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     9121 aagacaatag caggcatgct ggggatgcgg tgggctctat ggttaattaa ccagtcaagt
     9181 cagctacttg gcgagatcga cttgtctggg tttcgactac gctcagaatt gcgtcagtca
     9241 agttcgatct ggtccttgct attgcacccg ttctccgatt acgagtttca tttaaatcat
     9301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     9361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9781 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    10021 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    10081 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    10141 acctatctca gcgatctgtc tatttcgttc atccatagtt gcatttaaat ttccgaactc
    10201 tccaaggccc tcgtcggaaa atcttcaaac ctttcgtccg atccatcttg caggctacct
    10261 ctcgaacgaa ctatcgcaag tctcttggcc ggccttgcgc cttggctatt gcttggcagc
    10321 gcctatcgcc aggtattact ccaatcccga atatccgaga tcgggatcac ccgagagaag
    10381 ttcaacctac atcctcaatc ccgatctatc cgagatccga ggaatatcga aatcggggcg
    10441 cgcctggtgt accgagaacg at
//
