LOCUS       sequence-438456-        8901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation transfer plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, 
            Piqani B, Eisenhaure TM, Luo B, Grenier JK, Carpenter AE, Foo SY, 
            Stewart SA, Stockwell BR, Hacohen N, Hahn WC, Lander ES, Sabatini 
            DM, Root DE
  TITLE     A lentiviral RNAi library for human and mouse genes applied to an 
            arrayed viral high-content screen.
  JOURNAL   Cell. 2006 Mar 24. 124(6):1283-98.
  PUBMED    16564017
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Mar 24. 124(6):1283-98."
COMMENT     Sequence Label: pLKO.1 - TRC cloning vector
FEATURES             Location/Qualifiers
     source          1..8901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(17..34)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(188..776)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(268..287)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(947..1738,1739..1807))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1570..1589
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1808..1912)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1938..2393)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2075..2096)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2287..2306
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2520..2542
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2534..2551
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2535..2551
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2561..2580
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2561..2579
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2600..2735
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2775..2896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2786..2805)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2840..2859
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             2968..3201
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(3329..3928)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3412..3432)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3909..3928
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3950..4460)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4013..4031)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    4509..4626
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(6540..6788)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(6598..6617)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(6768..6788)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(7086..7130)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7315..7548
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8041..8166
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8213..8393
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8394..8620)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(8648..8668)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8648..8666)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8687..8703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8687..8703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8700..8722)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8711..8727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8735..8741,8742..8759,8760..8765))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8780..8801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
       61 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      121 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      181 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      241 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      301 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      361 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      421 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      481 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      541 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      601 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      661 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      721 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      781 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      841 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      901 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
      961 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1021 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1081 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1141 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1201 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1261 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1321 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1381 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1441 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     1501 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     1561 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     1621 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1681 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     1741 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     1801 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1861 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1921 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     1981 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     2041 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     2101 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     2161 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     2221 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     2281 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     2341 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     2401 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     2461 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     2521 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     2581 cgaattgggt acgtccctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     2641 gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     2701 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggatagct
     2761 agagccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     2821 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     2881 ctgcaataaa caagttcctc tcactctctg atattcattt ctttgcaagt tataaatact
     2941 gaataataag atgacatgaa ctactactgc tagagatttt ccacactgac taaaagggtc
     3001 tgagggatct ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc
     3061 aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg
     3121 ctcagatctg gtctaaccag agagacccag tacaagcaaa aagcagatct tgtcttcgtt
     3181 gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta agatctacag
     3241 ctgccttgta agtcattggt cttaaaggta ccgatgcatg gggtcgtgcg ctcctttcgg
     3301 tcgggcgctg cgggtcgtgg ggcgggcgtc aggcaccggg cttgcgggtc atgcaccagg
     3361 tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga
     3421 aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg cgctcggccg
     3481 cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg tcgggcgaga
     3541 cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc aggaggcctt
     3601 ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc gggccgatct
     3661 cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc accgcggcgc
     3721 cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg aagagttctt
     3781 gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc gtggcggggt
     3841 agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg cgggtggcga
     3901 ggcgcaccgt gggcttgtac tcggtcatgg taagctccgg tggatccccc tggggagaga
     3961 ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga ggcttgcaga
     4021 atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac cccgcctccc
     4081 gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg ctattggcca
     4141 cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct gcgagggtac
     4201 tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt
     4261 cccgacttag gggcggagca ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga
     4321 tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc
     4381 gtttcccgga accacgccca gagcagccgc gtccctgcgc aaacccaggg ctgccttgga
     4441 aaaggcgcaa ccccaacccc ctcgagccgc ggccaaagtg gatctctgct gtccctgtaa
     4501 taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct
     4561 gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt
     4621 ttaaaattgt ggatgaatac tgccatttgt ctcgaggtcg agaattctac aaaaaagagc
     4681 aagaagctaa aaaagattta aaaattattt ttagcgcagt taatggaaca ggaactaaat
     4741 ttaccccaaa aatattacgt gaatcaggat ataacgttat tgaggttgaa gagcatgcat
     4801 ttgaagatga aacatttaaa aatgttgtaa atccaaatcc agaatttgat cctgcatgaa
     4861 aaataccgct tgaatatggt attaaacatg atgcagatat tattattatg aatgacccag
     4921 atgctgacag atttggaatg gcaataaaac atgatggtca ttttgtaaga ttagatggaa
     4981 atcaaacagg accaatttta attgattgaa aattatcaaa tctaaaacgc ttaaatagca
     5041 ttccaaaaaa tccggctcta tattcaagtt ttgtaacaag tgatttgggt gatagaatcg
     5101 ctcatgaaaa atatggagtt aatattgtaa aaactttaac tggatttaaa tgaatgggta
     5161 gagaaattgc taaagaagaa gataacggat taaattttgt ttttgcttat gaagaaagtt
     5221 atggatatgt aattgatgac tcagctagag ataaagatgg aatacaagct tctatattaa
     5281 tagcagaggc tgcttgattt tataaaaaac aaaataaaac attagtagac tatttagaag
     5341 atttatttaa agaaatgggt gcatattaca ctttcacttt aaacttgaat tttaaaccag
     5401 aagaaaagaa attaaaaatt gaaccattaa tgaaatcatt gagagcaaca cccttaactc
     5461 aaattgctgg acttaaagtt gttaatgttg aagactacat cgatggaatg tataatatgc
     5521 caggacaaga cttactaaaa ttttatttag aagataagtc atgatttgct gttcgcccaa
     5581 gtggaactga acctaaacta aaaatttatt ttataggtgt tggtgaatct gttcaaaacg
     5641 ctaaagttaa agtagacgaa attattaaag aattaaaatt aaaaatgaat atataggaga
     5701 aaaaatgaaa ctaaacaaat atatagatca cacattatta aaacaagatg ctacgaaagc
     5761 tgaaattaaa caattatgtg atgaagcaat tgaatttgat tttgcaacag tttgtgttaa
     5821 ttcatattga acaagctatt gtaaagaatt attaaaaggc acaaatgtag gaataacaaa
     5881 tgttgtaggt tttcctctag gtgcatgcac aacagctaca aaagcattcg aagtttctga
     5941 agcaattaaa gatggtgcaa cagaaattga tatggtatta aatattggtg cattaaaaga
     6001 caaaaattat gaattagttt tagaagacat gaaagctgta aaaaaagcag ctggatcaca
     6061 tgttgttaaa tgtattatgg aaaattgttt attaacaaaa gaagaaatca tgaaagcttg
     6121 tgaaatagct gttgaagctg gattagaatt tgttaaaaca tcaacaggat tttcaaaatc
     6181 aggtgcaaca tttgaagatg ttaaactaat gaagtcagtt gttaaagaca atgctttagt
     6241 taaagcagct ggtggagtta gaacatttga agatgctcaa aaaatgattg aagcaggagc
     6301 tgaccgctta ggaacaagtg gtggagtagc tattattaaa ggtgaagaaa acaacgcgag
     6361 ttactaaaac tagcgttttt ttattttgct catttttatt aaaagtttgc aaaaaggaac
     6421 ataaaaattc taattattga tactaaagtt attaaaaaga agattttggt tgattttata
     6481 aaggtcatag aatataatat tttagcatgt gtattttgtg tgctcattta caacacaccg
     6541 gtgtttcgtc ctttccacaa gatatataaa gccaagaaat cgaaatactt tcaagttacg
     6601 gtaagcatat gatagtccat tttaaaacat aattttaaaa ctgcaaacta cccaagaaat
     6661 tattactttc tacgtcacgt attttgtact aatatctttg tgtttacagt caaattaatt
     6721 ccaattatct ctctaacagc cttgtatcgt atatgcaaat atgaaggaat catgggaaat
     6781 aggccctcgg tgaagggggc ggccgctcga ggctagtctc gtgatcgata ccgtcgagat
     6841 ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct
     6901 tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat
     6961 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag
     7021 cctcctacta tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa
     7081 ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg
     7141 ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc
     7201 cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca
     7261 ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc
     7321 tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc
     7381 cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc
     7441 tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg
     7501 cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct
     7561 cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca
     7621 atttttacta ctttatattt atataattca cttctccaat tgtccctcat atctcctcct
     7681 ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc
     7741 ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata
     7801 gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg
     7861 ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac
     7921 tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt
     7981 tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact
     8041 gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact
     8101 caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg
     8161 agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag
     8221 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     8281 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     8341 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccgtttatg
     8401 tatcgagcta ggcacttaaa tacaatatct ctgcaatgcg gcaattcagt ggttcgtcca
     8461 atccatgtca gacccgtctg ttgccttcct aataaggcac gatcgtacca ccttacttcc
     8521 accaatcggc atgcacggtg ctttttctct ccttgtaagg catgttgcta actcatcgtt
     8581 accatgttgc aagactacaa gagtattgca taagactaca ttaagcttgc agctccagct
     8641 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     8701 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8761 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8821 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8881 ggagaggcgg tttgcgtatt g
//
