LOCUS       sequence-438474-        9002 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle vector for baculovirus production, using FlashBac bacmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Wang J, Choi JM, Holehouse AS, Lee HO, Zhang X, Jahnel M, Maharana 
            S, Lemaitre R, Pozniakovsky A, Drechsel D, Poser I, Pappu RV, 
            Alberti S, Hyman AA
  TITLE     A Molecular Grammar Governing the Driving Forces for Phase 
            Separation of Prion-like RNA Binding Proteins.
  JOURNAL   Cell. 2018 Jul 26;174(3):688-699.e16. doi: 
            10.1016/j.cell.2018.06.006. Epub 2018 Jun 28.
  PUBMED    29961577
REFERENCE   2  (bases 1 to 9002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.06.006"; journalName: "Cell"; date: 
            "2018-07-26- 26"; volume: "174"; issue: "3"; pages: "688-699.e16"
COMMENT     Sequence Label: 
            TH1263-018_Jie_SFPQ_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP
FEATURES             Location/Qualifiers
     source          1..9002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        79..170
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     95..112
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     regulatory      212..221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             230..247
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             260..1360
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1334..1357
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1415..1438
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3596..3616
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(3617..3619,3620..3622,3623..4336)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3662..3683)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3923..3942)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4270..4291
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4469..4603
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4506..4525)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4560..4579
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     3'UTR           4676..5048
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     primer_bind     complement(5400..5417)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5510..6098)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5590..5609)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6269..7060,7061..7129))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6892..6911
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7130..7234)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7535..7557
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7549..7566
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7550..7566
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7577..7596
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7577..7595
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8206..8330
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(8368..8973)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 agctgccacc attttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat
       61 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
      121 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
      181 attcataccg tcccaccatc gggcgcggat cgccaccatg ggcagcagcc atcaccatca
      241 ccatcacagc agcggcagaa tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg
      301 cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat
      361 taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac
      421 tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc
      481 tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac
      541 ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt
      601 atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc
      661 ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga
      721 accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa
      781 cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac
      841 cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc
      901 agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc
      961 caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca
     1021 accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa
     1081 agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt
     1141 taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggtgaa
     1201 agatccgcgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat
     1261 cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg
     1321 tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa
     1381 caacaataac aataacaaca acagcagcgg cagactggaa gttctgttcc aggggccggc
     1441 ggccgcaatg agcagagacc gtttcagaag caggggtggt ggtggtggtg gtttccatag
     1501 aaggggaggt ggtggtggca ggggtggttt gcatgacttc cgcagccctc ccccaggcat
     1561 gggactgaac cagaaccgtg gtcctatggg tccaggtcca ggacagtcag gaccaaagcc
     1621 tcccatccca cctcccccac ctcaccagca acaacagcag cccccacctc agcaaccccc
     1681 acctcagcag cccccacctc accaaccccc acctcaccct caacctcatc aacaacaaca
     1741 acccccacct cccccacagg actccagcaa gcctgtggtg gctcagggtc ctggaccagc
     1801 cccaggagtc ggttctgccc ctcccgcttc ttcatctgcc ccacctgcta ctcccccaac
     1861 ttctggtgct cctcccggat ctggaccagg acctactcca accccacctc ccgctgtgac
     1921 ttctgctcca cctggtgctc ctcctcctac ccctcctagc tctggtgtcc caaccactcc
     1981 tccccaggcc ggtggcccac ctcccccacc tgctgctgtg cctggtcctg gaccaggtcc
     2041 taagcaagga ccaggaccag gaggtccaaa gggcggaaag atgcctggtg gccctaagcc
     2101 cggaggtggc cctggactga gcactcccgg aggtcaccca aagcccccac acaggggcgg
     2161 aggtgagcca cgcggcggac gtcagcacca ccctccctac caccaacagc accaccaggg
     2221 accacctccc ggtggccccg gaggtcgtag cgaggaaaag atctcagact ccgaaggctt
     2281 caaggctaac ctgtctctgc tgcgccgtcc cggagagaag acttacaccc agcgctgccg
     2341 tctgttcgtc ggtaacctgc cagctgacat caccgaggac gaattcaaga ggctgttcgc
     2401 caagtacgga gaaccaggag aggtgttcat caacaagggc aagggcttcg gattcatcaa
     2461 gctggaatcc agagccctgg ctgaaatcgc caaggctgag ctggacgaca ctcctatgag
     2521 gggcagacag ctgcgcgtcc gtttcgctac ccacgctgcc gctctgtcag tgcgcaacct
     2581 gagcccatac gtctctaacg aactgctgga ggaagctttc tcccagttcg gtcctatcga
     2641 gcgtgccgtg gtcatcgtgg acgacagggg cagatcaact ggcaagggca tcgtcgagtt
     2701 cgcttccaag cccgccgctc gcaaggcctt cgaacgttgc tcagagggag tgttcctgct
     2761 gaccactacc ccacgccctg tgatcgtcga accactggag cagctggacg acgaagacgg
     2821 tctgcccgag aagctggctc agaagaaccc aatgtaccag aaggagaggg aaactccacc
     2881 tagattcgcc cagcacggta ccttcgagta cgaatactca cagaggtgga agtccctgga
     2941 cgagatggaa aagcagcaga gagaacaggt ggagaagaac atgaaggacg ctaaggacaa
     3001 gctggagtcc gaaatggagg acgcttacca cgagcaccag gccaacctgc tgcgtcagga
     3061 cctgatgagg agacaggagg aactgcgccg tatggaggaa ctgcacaacc aggaaatgca
     3121 gaagcgcaag gagatgcagc tgcgtcagga ggaagagagg agacgccgtg aagaggaaat
     3181 gatgatccgc cagcgtgaaa tggaggaaca gatgaggaga cagagggagg aaagctactc
     3241 tagaatgggt tacatggacc ctagggagag agacatgcgc atgggcggag gtggcgctat
     3301 gaacatgggc gacccctacg gatcaggagg tcaaaagttc cctcctctgg gtggtggtgg
     3361 tggtatcggc tacgaagcta accctggagt ccctcccgcc actatgtcag gttccatgat
     3421 gggctccgac atgaggaccg agagattcgg acagggtggc gccggtcctg tgggaggtca
     3481 aggacctagg ggaatgggtc ctggaacccc agcaggttac ggtagaggaa gggaggaata
     3541 cgaaggtcca aacaagaagc caaggttcgg cgcgccggga tccagcagcg gcagagaaaa
     3601 cttgtatttc cagggcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     3661 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     3721 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     3781 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     3841 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     3901 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     3961 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     4021 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     4081 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     4141 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     4201 gctgcccgac aaccactacc tgagcaccca gtccaaactg agcaaagacc ccaacgagaa
     4261 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     4321 cgagctgtac aagtaataga agcttgtcga gaagtactag aggatcataa tcagccatac
     4381 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     4441 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     4501 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     4561 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac tgcttgagcc
     4621 taggagatcc gaaccagata agtgaaagcc tgcatccgca gatcttaatt aataaaacac
     4681 gatacattgt tattagtaca tttattaagc gctagattct gtgcgttgtt gatttacaga
     4741 caattgttgt acgtatttta ataattcatt aaatttataa tctttagggt ggtatgttag
     4801 agcgaaaatc aaatgatttt cagcgtcttt atatctgaat ttaaatatta aatcctcaat
     4861 agatttgtaa aataggtttc gattagtttc aaacaagggt tgtttttccg aaccgatggc
     4921 tggactatct aatggatttt cgctcaacgc cacaaaactt gccaaatctt gtagcagcaa
     4981 tctagctttg tcgatattcg tttgtgtttt gttttgtaat aaaggttcga cgtcgttcaa
     5041 aatattatgc gcttttgtat ttctttcatc actgtcgtta gtgtacaatt gactcgacgt
     5101 aaacacgtta aataacgctt ggacatattt aacatcgggc gtgttagctt tattaggccg
     5161 attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct tccgaagacg attttgccat
     5221 agccacacga cgcctattaa ttgtgtcggc taacacgtcc gcgatcaaat ttgtagttga
     5281 gctttttggg cctcatgggc cttccgctca ctgcccgctt tccagtcggg aaacctgtcg
     5341 tgccagctgc attaacatgg tcatagctgt ttccttgcgt attgggcgct ctccgcttcc
     5401 tcgctcactg actcgctgcg ctcggtcgtt cgggtaaagc ctggggtgcc taatgagcaa
     5461 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5521 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5581 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5641 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5701 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5761 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5821 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5881 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5941 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6001 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6061 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6121 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6181 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6241 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6301 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6361 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gaaccacgct
     6421 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6481 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6541 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6601 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6661 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6721 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     6781 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6841 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     6901 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     6961 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7021 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7081 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7141 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7201 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
     7261 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     7321 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     7381 agggttgagt ggccgctaca gggcgctccc attcgccatt caggctgcgc aactgttggg
     7441 aagggcgttt cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     7501 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     7561 gccagtgagc gcgacgtaat acgactcact atagggcgaa ttggcggaag gccgtcaagg
     7621 ccgtgacgtt aaaactatta agccatccaa tcgaccgtta gtcgaatcag gaccgctggt
     7681 gcgagaagcc gcgaagtatg gcgaatgcat cgtataacgt gtggagtccg ctcattagag
     7741 cgtcatgttt agacaagaaa gctacatatt taattgatcc cgatgatttt attgataaat
     7801 tgaccctaac tccatacacg gtattctaca atggcggggt tttggtcaaa atttccggac
     7861 tgcgattgta catgctgtta acggctccgc ccactattaa tgaaattaaa aattccaatt
     7921 ttaaaaaacg cagcaagaga aacatttgta tgaaagaatg cgtagaagga aagaaaaatg
     7981 tcgtggacat gctgaacaac aagattaata tgcctccgtg tataaaaaaa atattgaacg
     8041 atttgaaaga aaacaatgta ccgcgcggcg gtatgtacag gaagaggttt atactaaact
     8101 gttacattgc aaacgtggtt tcgtgtgcca agtgtgaaaa ccgatgttta atcaaggctc
     8161 tgacgcattt ctacaaccac gactccaagt gtgtgggtga agtcatgcat cttttaatca
     8221 aatcccaaga tgtgtataaa ccaccaaact gccaaaaaat gaaaactgtg gacaagctct
     8281 gtccgtttgc tggcaactgc aagggtctca atcctatttg taattattga ataataaaac
     8341 aattataaat gctaaatttg ttttttatta acgatacaaa ccaaacgcaa caagaacatt
     8401 tgtagtatta tctataattg aaaacgcgta gttataatcg ctgaggtaat atttaaaatc
     8461 attttcaaat gattcacagt taatttgcga caatataatt ttattttcac ataaactaga
     8521 cgccttgtcg tcttcttctt cgtattcctt ctctttttca tttttctcct cataaaaatt
     8581 aacatagtta ttatcgtatc catatatgta tctatcgtat agagtaaatt ttttgttgtc
     8641 ataaatatat atgtcttttt taatggggtg tatagtaccg ctgcgcatag tttttctgta
     8701 atttacaaca gtgctatttt ctggtagttc ttcggagtgt gttgctttaa ttattaaatt
     8761 tatataatca atgaatttgg gatcgtcggt tttgtacaat atgttgccgg catagtacgc
     8821 agcttcttct agttcaatta caccattttt tagcagcacc ggattaacat aactttccaa
     8881 aatgttgtac gaaccgttaa acaaaaacag ttcacctccc ttttctatac tattgtctgc
     8941 gagcagttgt ttgttgttaa aaataacagc cattgtaatg agacgcacaa actaatatca
     9001 ca
//
