LOCUS       sequence-438476-        8849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle vector for baculovirus production, using FlashBac bacmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Wang J, Choi JM, Holehouse AS, Lee HO, Zhang X, Jahnel M, Maharana 
            S, Lemaitre R, Pozniakovsky A, Drechsel D, Poser I, Pappu RV, 
            Alberti S, Hyman AA
  TITLE     A Molecular Grammar Governing the Driving Forces for Phase 
            Separation of Prion-like RNA Binding Proteins.
  JOURNAL   Cell. 2018 Jul 26;174(3):688-699.e16. doi: 
            10.1016/j.cell.2018.06.006. Epub 2018 Jun 28.
  PUBMED    29961577
REFERENCE   2  (bases 1 to 8849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.06.006"; journalName: "Cell"; date: 
            "2018-07-26- 26"; volume: "174"; issue: "3"; pages: "688-699.e16"
COMMENT     Sequence Label: 
            TH1259-014_Jie_EWSR1_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP
FEATURES             Location/Qualifiers
     source          1..8849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        79..170
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     95..112
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     regulatory      212..221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             230..247
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             260..1360
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1334..1357
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1415..1438
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3443..3463
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(3464..3466,3467..3469,3470..4183)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3509..3530)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3770..3789)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4117..4138
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4316..4450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4353..4372)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4407..4426
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     3'UTR           4523..4895
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     primer_bind     complement(5247..5264)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5357..5945)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5437..5456)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6116..6907,6908..6976))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6739..6758
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6977..7081)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7382..7404
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7396..7413
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7397..7413
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7424..7443
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7424..7442
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8053..8177
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(8215..8820)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 agctgccacc attttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat
       61 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
      121 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
      181 attcataccg tcccaccatc gggcgcggat cgccaccatg ggcagcagcc atcaccatca
      241 ccatcacagc agcggcagaa tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg
      301 cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat
      361 taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac
      421 tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc
      481 tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac
      541 ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt
      601 atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc
      661 ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga
      721 accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa
      781 cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac
      841 cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc
      901 agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc
      961 caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca
     1021 accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa
     1081 agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt
     1141 taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggtgaa
     1201 agatccgcgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat
     1261 cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg
     1321 tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa
     1381 caacaataac aataacaaca acagcagcgg cagactggaa gttctgttcc aggggccggc
     1441 ggccgcaatg gcgtccacag attactccac ctactcgcaa gcagcagcac agcaaggcta
     1501 ctcagcatac acggcacaac caactcaagg atacgctcag accactcagg cctacggcca
     1561 gcagagctac ggaacttacg gtcagccaac cgacgtgtct tacactcagg ctcagaccac
     1621 tgccacctac ggtcagactg cttacgctac ctcatacgga cagcctccca ccggatacac
     1681 cactccaact gctcctcagg cctactccca gcctgtgcag ggctacggaa ctggtgctta
     1741 cgacaccact accgccaccg tcactaccac tcaggcttca tacgctgccc agtccgccta
     1801 cggaactcag cccgcttacc cagcctacgg tcagcagcct gctgctactg ctcctactcg
     1861 tccccaggac ggaaacaagc ctaccgagac ttcacagccc cagtccagca ccggtggcta
     1921 caaccagccc tccctgggct acggacagtc aaactactcc tacccacagg tgcctggttc
     1981 ctaccccatg cagccagtca ccgccccacc tagctaccct cctacttctt actcttcaac
     2041 ccagccaact tcatacgacc agtccagcta ctcccagcag aacacctacg gacagccttc
     2101 ttcatacggt cagcagtcca gctacggcca gcagtcttca tacggacagc agcctcccac
     2161 cagctaccca cctcagactg gatcatactc ccaggctccc agccagtact ctcagcagtc
     2221 cagctcttac ggtcagcagt catccttccg ccaggaccac ccaagctcta tgggcgtgta
     2281 cggacaggag agcggaggtt tctctggtcc tggcgaaaac agaagcatgt ctggacctga
     2341 caaccgcgga cgtggtaggg gcggattcga ccgcggtggc atgtctcgtg gaggtagggg
     2401 cggaggtcgt ggcggaatgg gttctgctgg cgagaggggt ggcttcaaca agcctggagg
     2461 tcccatggac gaaggtccag acctggacct gggtcctcct gtcgaccctg acgaggactc
     2521 agacaactcc gctatctacg tgcagggtct gaacgactcc gtcactctgg acgacctggc
     2581 cgacttcttc aagcagtgcg gcgtggtcaa gatgaacaag cgtaccggac agcccatgat
     2641 ccacatctac ctggacaagg aaaccggaaa gccaaagggt gacgctactg tgagctacga
     2701 ggaccctccc actgctaagg ctgctgtcga atggttcgac ggcaaggact tccagggctc
     2761 aaagctgaag gtgtccctgg ctagaaagaa gccacctatg aactctatgc gcggcggact
     2821 gcccccaaga gaaggaaggg gaatgcctcc cccactgagg ggtggcccag gaggtcctgg
     2881 cggacccggt ggcccaatgg gaaggatggg aggtagaggc ggagaccgcg gtggcttccc
     2941 tccccgtgga ccaaggggta gcagaggcaa cccatctgga ggtggcaacg tgcaacacag
     3001 ggctggtgac tggcagtgcc caaacccagg ttgcggcaac cagaacttcg cttggaggac
     3061 cgagtgcaac cagtgcaagg cccctaagcc cgaaggtttc ctgccacctc ccttcccacc
     3121 tcccggaggt gacagaggcc gcggcggacc tggtggcatg cgcggaggtc gtggcggact
     3181 gatggacaga ggtggccccg gaggtatgtt caggggcgga agaggtggcg accgtggagg
     3241 tttccgcggc ggacgtggaa tggacagggg tggcttcgga ggtggccgcc gtggaggtcc
     3301 aggcggacca cctggtcctc tgatggaaca gatgggtggc aggagaggag gtcgcggtgg
     3361 accaggaaaa atggataaag gagaacacag acaggagcgt cgtgacagac cctacggcgc
     3421 gccgggatcc agcagcggca gagaaaactt gtatttccag ggcatggtga gcaagggcga
     3481 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3541 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3601 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     3661 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3721 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     3781 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3841 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3901 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     3961 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     4021 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     4081 caaactgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4141 cgccgccggg atcactctcg gcatggacga gctgtacaag taatagaagc ttgtcgagaa
     4201 gtactagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     4261 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     4321 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4381 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4441 tgtctggatc tgatcactgc ttgagcctag gagatccgaa ccagataagt gaaagcctgc
     4501 atccgcagat cttaattaat aaaacacgat acattgttat tagtacattt attaagcgct
     4561 agattctgtg cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa
     4621 tttataatct ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata
     4681 tctgaattta aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa
     4741 caagggttgt ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac
     4801 aaaacttgcc aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt
     4861 ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact
     4921 gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat aacgcttgga catatttaac
     4981 atcgggcgtg ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga
     5041 agttgcttcc gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa
     5101 cacgtccgcg atcaaatttg tagttgagct ttttgggcct catgggcctt ccgctcactg
     5161 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aacatggtca tagctgtttc
     5221 cttgcgtatt gggcgctctc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5281 gtaaagcctg gggtgcctaa tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5341 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5401 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5461 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5521 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5581 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5641 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5701 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     5761 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     5821 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5881 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5941 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6001 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6061 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6121 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6181 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6241 ctgcaatgat accgcgagaa ccacgctcac cggctccaga tttatcagca ataaaccagc
     6301 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6361 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6421 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6481 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6541 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6601 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6661 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6721 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6781 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6841 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6901 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6961 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7021 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7081 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     7141 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     7201 ttataaatca aaagaataga ccgagatagg gttgagtggc cgctacaggg cgctcccatt
     7261 cgccattcag gctgcgcaac tgttgggaag ggcgtttcgg tgcgggcctc ttcgctatta
     7321 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     7381 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg acgtaatacg actcactata
     7441 gggcgaattg gcggaaggcc gtcaaggccg tgacgttaaa actattaagc catccaatcg
     7501 accgttagtc gaatcaggac cgctggtgcg agaagccgcg aagtatggcg aatgcatcgt
     7561 ataacgtgtg gagtccgctc attagagcgt catgtttaga caagaaagct acatatttaa
     7621 ttgatcccga tgattttatt gataaattga ccctaactcc atacacggta ttctacaatg
     7681 gcggggtttt ggtcaaaatt tccggactgc gattgtacat gctgttaacg gctccgccca
     7741 ctattaatga aattaaaaat tccaatttta aaaaacgcag caagagaaac atttgtatga
     7801 aagaatgcgt agaaggaaag aaaaatgtcg tggacatgct gaacaacaag attaatatgc
     7861 ctccgtgtat aaaaaaaata ttgaacgatt tgaaagaaaa caatgtaccg cgcggcggta
     7921 tgtacaggaa gaggtttata ctaaactgtt acattgcaaa cgtggtttcg tgtgccaagt
     7981 gtgaaaaccg atgtttaatc aaggctctga cgcatttcta caaccacgac tccaagtgtg
     8041 tgggtgaagt catgcatctt ttaatcaaat cccaagatgt gtataaacca ccaaactgcc
     8101 aaaaaatgaa aactgtggac aagctctgtc cgtttgctgg caactgcaag ggtctcaatc
     8161 ctatttgtaa ttattgaata ataaaacaat tataaatgct aaatttgttt tttattaacg
     8221 atacaaacca aacgcaacaa gaacatttgt agtattatct ataattgaaa acgcgtagtt
     8281 ataatcgctg aggtaatatt taaaatcatt ttcaaatgat tcacagttaa tttgcgacaa
     8341 tataatttta ttttcacata aactagacgc cttgtcgtct tcttcttcgt attccttctc
     8401 tttttcattt ttctcctcat aaaaattaac atagttatta tcgtatccat atatgtatct
     8461 atcgtataga gtaaattttt tgttgtcata aatatatatg tcttttttaa tggggtgtat
     8521 agtaccgctg cgcatagttt ttctgtaatt tacaacagtg ctattttctg gtagttcttc
     8581 ggagtgtgtt gctttaatta ttaaatttat ataatcaatg aatttgggat cgtcggtttt
     8641 gtacaatatg ttgccggcat agtacgcagc ttcttctagt tcaattacac cattttttag
     8701 cagcaccgga ttaacataac tttccaaaat gttgtacgaa ccgttaaaca aaaacagttc
     8761 acctcccttt tctatactat tgtctgcgag cagttgtttg ttgttaaaaa taacagccat
     8821 tgtaatgaga cgcacaaact aatatcaca
//
