LOCUS       sequence-438477-       14838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional reporter for ARF7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14838)
  TITLE     Bishopp Lab ARF7 Reporter Plasmids 2024
REFERENCE   2  (bases 1 to 14838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: int>pro::GFP
FEATURES             Location/Qualifiers
     source          1..14838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..68
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     rep_origin      195..630
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     CDS             921..1709
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1880..2468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2369..2388
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    2559..2583
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(2679..3386)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    3405..3429
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5232..5287
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    complement(5309..5333)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(10691..10693,10694..10696,10697..11407)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(10736..10757)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10997..11016)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11344..11365
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      11716..12357
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             13040..14068
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      14076..14783
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(14816..14834)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(14817..14834)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tctagaccaa gcatgcacag ctggcgttct cagccagatc caacgttaca ccacaatata
       61 tcctgccaag atctaattcc ggggatcgga aatccagaag cccgagaggt tgccgccttt
      121 cgggcttttt ctttttcaaa aaaaaaaatt tataaaacga tctgttgcgg ccggccgccg
      181 ggttgtgggc aaaggcgctc gacggtgggc aaccgcttgc ggttgtccac gggcggagcc
      241 ggtgcgcgta gcgcattgtc cacaagccaa gggcgaccaa taattgatat atatattcat
      301 aattgaaaag ctaattgaac atactacttg ctgtaactac ttgccggagc gaggggtgtt
      361 tgcaagctgt tgatctgaaa gggctattag cgttctcacg tgcctttttg attagcgatt
      421 tcacgtgacc ttattagcga tttcacgtac tccgattagc gatttcacgt accctgatta
      481 gcgatttcac gtggatagtt tttggagcgg gccggaaagc cccgtgaatc aaggctttgc
      541 ggggcattag cggtttcacg tggataacta ccctctatcc acaggcttcc ggggataaaa
      601 aagcccgctc gacggcgggc tgttggatgg ggatcgcctg aatcgcccca tcatccagcc
      661 agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt
      721 tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct
      781 tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat
      841 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
      901 acagtaatac aaggggtgtt atgagggaag cggtgatcgc cgaagtatcg actcaactat
      961 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     1021 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     1081 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     1141 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     1201 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     1261 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     1321 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     1381 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     1441 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     1501 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     1561 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct tatcttggac
     1621 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac tacgtgaaag
     1681 gcgagatcac caaggtagtc ggcaaataat cactagacca atgttacaca tatatacttt
     1741 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1801 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1861 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1921 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttc
     1981 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2041 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2101 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2161 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2221 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     2281 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2341 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2401 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2461 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2521 ctcacatgag atctcaaaca aacacataca gcgacttagt ttacccgcca atatatcctg
     2581 tcaaggctcg aactaggctc ggacgaagta agcggccgca accttaatta aacacggcgc
     2641 gccaacagcg atcgcaccag gtaccttgca tgccggtcct gctgagcctc gacatgttgt
     2701 cgcaaaattc gccctggacc cgcccaacga tttgtcgtca ctgtcaaggt ttgacctgca
     2761 cttcatttgg ggcccacata caccaaaaaa atgctgcata attctcgggg cagcaagtcg
     2821 gttacccggc cgccgtgctg gaccgggttg aatggtgccc gtaactttcg gtagagcgga
     2881 cggccaatac tcaacttcaa ggaatctcac ccatgcgcgc cggcggggaa ccggagttcc
     2941 cttcagtgag cgttattagt tcgccgctcg gtgtgtcgta gatactagcc cctggggcac
     3001 ttttgaaatt tgaataagat ttatgtaatc agtcttttag gtttgaccgg ttctgccgct
     3061 ttttttaaaa ttggatttgt aataataaaa cgcaattgtt tgttattgtg gcgctctatc
     3121 atagatgtcg ctataaacct attcagcaca atatattgtt ttcattttaa tattgtacat
     3181 ataagtagta gggtacaatc agtaaattga acggagaata ttattcataa aaatacgata
     3241 gtaacgggtg atatattcat tagaatgaac cgaaaccggc ggtaaggatc tgagctacac
     3301 atgctcaggt tttttacaac gtgcacaaca gaattgaaag caaatatcat gcgatcatag
     3361 gcgtctcgca tatctcatta aagcaggact ctagaggatc atcaaccact ttgtacaaga
     3421 aagctgggtc ggcgcgccca cccttttact tgtacaactc gtccataccg tagaggaaga
     3481 ggtggtggcg gccctcggaa cgctcgtact gttcgacaat ggtgtaatcc tcgttgtggg
     3541 aggtaatgtc caacttggta tcaacgtagt aataaccagg caattgaacg ggtttcttgg
     3601 ccatgtagat ggtcttaaac tccacgaggt aatgtccacc gtccttcaac ttcaatgcct
     3661 ggtgaatctc gcccttcaac actccatcgc ggggatacaa gcgctctgtt gaagcctccc
     3721 agcccatagt cttcttctgc ataactgggc cgtctggtgg gaaattggtt ccacgcattt
     3781 tgaccttgta gataagcgta ccatcttgaa gggaggagtc ttgggtgact gtaaccaaac
     3841 caccgtcctc gaagttcata acgcgctccc acttaaatcc ttctgggaaa gacaacttct
     3901 tataatctgg aatatcggct gggtgtttca cgtaagcctt ggaaccgtac ataaactgtg
     3961 gtgagagaat atcccaagcg aaaggaagtg gtccaccctt ggtgactttc aacttagctg
     4021 tctgagtgcc ctcatatggg cggccctcac cctctccttc gatctcgaac tcgtggccat
     4081 tcattgagcc ctccatacgg actttgaagc acataaactc cttgatcaca gccatgttat
     4141 tatcctcgga tgaagcggtg ccagagctgc cactaccggt gcttccagta ccatgaccca
     4201 agaaaaggtg atggcgaccc tcagacctct catattgttc aacgatggtg tagtcctcgt
     4261 tgtgggaggt gatatcgagt ttagtgtcca cgtagtaata gccggggagt tgcacgggct
     4321 tcttagccat gtagatggtc ttgaactcca ccaagtagtg gcctccatcc ttaagtttga
     4381 gagcttggtg gatttcaccc ttaagaacgc cgtctcttgg gtaaagcctc tcagtggagg
     4441 cttcccaacc catagttttc ttttgcatga ctggaccatc gggaggaaag tttgtgcctc
     4501 tcatcttcac cttgtagatc aaagtgccgt cctgcaaact agaatcttgg gtcacagtca
     4561 caagacctcc atcctcgaag ttcatgaccc tttcccactt gaaaccctct gggaaggaca
     4621 actttttgta atcggggatg tcagcaggat gcttaacgta tgccttagag ccatacatga
     4681 attgggggga caagatgtcc caagcgaagg gcaaaggacc acccttagtc actttgagct
     4741 ttgcggtttg ggtgccctcg tagggtctac cttcaccctc gccctcgatc tcgaactcgt
     4801 gaccgttcat ggaaccttcc atcctaacct tgaatctcat gaactctttg ataacctcct
     4861 cacccttgga aaccatggat cgggctgccg cagcggcagc agccgcagca ggtttggtga
     4921 ggacataaat ggcgtatatg atcccaggaa tatacccaag tagcgtcaaa accaaacata
     4981 tccaaaactc aacctgcaac aaaattcata atttcaactt ttaatttcta gtgtggataa
     5041 atgtaaaaaa agaaccatat ttgactaaag tataagatta attaaccaaa ataaaactca
     5101 gtctaaatat gagaggaaag ataaataccc cgcaaccaaa tctgagaaag acaccgagtg
     5161 gaggcaagag gatggcgata ataatatcaa cgaaagtagc tgtactcatt ttggtgaagg
     5221 gggcggccgc tgtaattgta aatagtaatt gtaatgttgt ttgttgtttg ttgttgttgg
     5281 taattgttgt aaaaatacgc ggccgcggag cctgcttttt tgtacaaact tgttgatgat
     5341 ccctgttaat cagaaaaact cagattaatc gacaaattcg atcgcacaaa ctagaaacta
     5401 acaccagatc tagatagaaa tcacaaatcg aagagtaatt attcgacaaa actcaaatta
     5461 tttgaacaaa tcggatgata tctatgaaac cctaatcgag aattaagatg atatctaacg
     5521 atcaaaccca gaaaatcgtc ttcgatctaa gattaacaga atctaaacca aagaacatat
     5581 acgaaattgg gatcgaacga aaacaaaatc gaagattttg agagaataag gaacacagaa
     5641 atttaccttg atcacggtag agagaattga gagaaagttt ttaagatttt gagaaattga
     5701 aatctgaatt gtgaagaaga agagctcttt gggtattgtt ttatagaaga agaagaagaa
     5761 aagacgagga cgactaggtc acgagaaagc taaggcggtg aagcaatagc taataataaa
     5821 atgacacgtg tattgagcgt tgtttacacg caaagttgtt tttggctaat tgccttattt
     5881 ttaggttgag gaaaagtatt tgtgctttga gttgataaac acgactcgtg tgtgccggct
     5941 gcaaccactt tgacgccgtt tattactgac tcgtcgacaa ccacaatttc taacggtcgt
     6001 cataagatcc agccgttgag atttaacgat cgttacgatt tatatttttt tagcattatc
     6061 gttttatttt ttaaatatac ggtggagctg aaaattggca ataattgaac cgtgggtccc
     6121 actgcattga agcgtatttc gtattttcta gaattcttcg tgctttattt cttttccttt
     6181 ttgttttttt ttgccattta tctaatgcaa gtgggcttat aaaatcagtg aatttcttgg
     6241 aaaagtaact tctttatcgt ataacatatt gtgaaattat ccatttcttt taatttttta
     6301 gtgttattgg atatttttgt atgattattg atttgcatag gataatgact tttgtatcaa
     6361 gttggtgaac aagtctcgtt aaaaaaggca agtggtttgg tgactcgatt tattcttgtt
     6421 atttaattca tatatcaatg gatcttattt ggggcctggt ccatatttaa cactcgtgtt
     6481 cagtccaatg accaataata ttttttcatt aataacaatg taacaagaat gatacacaaa
     6541 acattctttg aataagttcg ctatgaagaa gggaacttat ccggtcctag atcatcagtt
     6601 catacaaacc tccatagagt tcaacatctt aaacaagaat atcctgatct gaagaatgtg
     6661 gaggctttag tcccttggat acttgggagg ctgtggaaga acagaaacga gctggtgctt
     6721 aaagggaggg aatttggaac caatgaggta ttagtaagga cacaagaaga tgcagatgag
     6781 tggattagaa ggaaagaggc tcagaatgta aggaaagcac caaccacaac accgtcggga
     6841 cagacgagaa acatgggaag ctccaccaca atcttgggtt aagtgcaact ttgatggggc
     6901 atggccaaca gaaggattaa aatgtggctt agggtgggtg cttcgcgatc atacagggaa
     6961 ggtgttatgg ttaggtgcaa gagctgtggt aaaagtaaga agcgtgctgg aagtagaagt
     7021 ggaggctctt agatgggctg tgctgtcatt atcccgattc aattatagga agatcatttt
     7081 tgaggtggat tctcagcaac ttgtgtcttt ggttacatga aagttatgct tgtcaagtct
     7141 caatccaatt atccaagaca taaagtatct acttagcaag tttgaggatt ttatgcttgt
     7201 gcatacaagc cgagaaggaa atggagtggc agatagaata gctaaggaat ctctttcttt
     7261 tgagaattat gatctaaagt tgtattctat tgtaccaatt tgggttaaaa gctctgttga
     7321 gctagactca tatgcatacc taagagatgt cgcaaaccag ctcatgtcac caaacaacaa
     7381 ttagagccaa tgtgaaatgg ggtgggagtg tgaccacgtc aggtgggatt tgccgtgaga
     7441 ggcccttctt tattgtttgt tcctttttct tcaattatta ttttcaataa taatgtaatt
     7501 aaacttatca tcaaatacaa atattcgata aattaaagga tcccgttgaa ggtttgtgtg
     7561 tttctcgtat ctgataaatg tcaatccttt agtagaagaa attcattgct ggatttgaat
     7621 agattcaagt tttaagtaga agaggtcaca catttcttca gaattgctgg atttgagaac
     7681 ctgaattgaa ttggtttcgg tcattttctg cttagagagt aagctaagtt actattgtat
     7741 tggtttataa agactgtggc ttttagttgg tttagagcag ttctctccta tcttgttggt
     7801 ttgataatag taactgaatg aagatcctag agattttaag taatcacagc tttgatgttg
     7861 tgtgaatgca ggagaaacgg gatcctctac aagaaatatg aatattgata acacataaga
     7921 atagcaaaat ttttgtaact attattaaaa ataatattcg agatttgtta accatagtat
     7981 aacatttgta aaccactaaa cataaaagta acgagaaaat ctcctaaagc aaagtaacgt
     8041 ttctctatat ttagcgtttt acaaaattta tgttaaaaaa ttatgtttag cgttttacaa
     8101 aaattatgct agaaaagttt atgtttagcg gaaatctaaa atcacttaaa acgcaaaaaa
     8161 ttaatcagta cgtagattat tttccacaac tctctcttac atacaaatta acaaataatg
     8221 catatcagaa tgacatagta aagtcgagaa aatcatggaa tcgtaacgaa acttcctcgt
     8281 ctctctctga taattcatcc aactcaagtg gtgtattttg tggttacatt tacatatatt
     8341 gtcaacacca actgtcgaat tcattaaatc ggctaaaata cgttgatgtg attctggaaa
     8401 tatctttagg acagtatatg agttattcat cttgttcatt ttccactttc cactgcggac
     8461 accatacgga cacacaaccg gttctgaaat gcgtcacgct cttcttcctt tcattccaac
     8521 tttaatacat gcatgaagtc gtagttcgga gttcatacta aatttcggtc actttgtaat
     8581 aagaaaacta ttaacatttt tctttttctt tttttaaacg ttagctaggc ctcaaagttt
     8641 cacaaaaaaa aaagaaaaaa aaaagctagg cgtcgaaatc cataacttca aaaggccttc
     8701 ttacattcat ccaatctcca aacttctgtc ttcacgcatt tattgctcag ggacatcatg
     8761 tcgctactca ctgccactta tttgaattga catccattca ttccatacat atatattacc
     8821 ttcacttaaa gttgtaagta tatagaattt taagcattcc tgccaaataa gttttggatc
     8881 gaacctaaac taacaaagag gttactgttt tgtaactttc ttttttgatg tggggtcatt
     8941 caaagggttt acatgcattt tacaatggtc tgtgatgaat atggaggttc agtctttggt
     9001 agataaaact tgttatgtgg ccttaaacca aatagattgt agtttgtaca atgacatatt
     9061 tatggttata ttcggttttc caatatgttt aggaatatat atcaccgttt ttttttgggt
     9121 agacatttta acgattatag aggaaaattt tgaatgatgg ctgtactggg aaaagagtgt
     9181 aagttgtgat catcatatgt aattttattt tgtgtgacat tataacactt cttccaaaag
     9241 acaaaagttt aggttaatta atataagaaa ccaataaaga atcatgaaca aagagagaac
     9301 ttctatcatt tttgatatga aatcgtccgt taaaatattg ttctttgtga tcgcatatgc
     9361 tgtatgtact tagatttgat caattaatca acatttacaa caaaataaga tcaattgttt
     9421 ataatgatga tctaagcgaa tttagatttg tttgttatta tagaactcat tactaaacat
     9481 ttaagttcca ttctcgttta aaacatgaac gctagtttat gaaactcaga accaaccatg
     9541 taaaactcca caaaatttct agtaaaatgc ttttgttcaa aataatgaaa atcataaaat
     9601 tttttgatga attatgaatc ctaaaatcta tctgaattaa atatagttaa ctacaaaaga
     9661 aattgccaaa catatatgta cgcactttga tcaaataact agctagtcag cttagtttgt
     9721 ttttttcaat acatccccta taaatctatg ttatccctaa tcaagtatta caataaatga
     9781 tacagtaata ataaccttaa tgaaaaaaac ttatcaaatt atcagagata aggaaaaaga
     9841 aaacactaat aaagttgttt ttttgtgtgt caaagttaaa attttagagg acaataataa
     9901 cattaagaaa ttaaatcgtg gccagtttgc tagtgaatat gtcggcttca tgagcataac
     9961 taaataggta taaaaaaata aaaatatcgg aaaaagaaaa gagaaggaaa aaaaaaggag
    10021 aggaagaaga aaaagctgtt cctgtgcttt agcagacctt ttggtagttg tcctggtcgc
    10081 tgatcatccc tttccgacaa ctctgttgct ctttctctca cgtttgagtc ttaatcttcg
    10141 attgtccttg gtcatcctta agttttgttt ttctctttcg attcattttc cagatttctt
    10201 tttatagaaa cccgtctctt tgatttgatt ccccccaaga aaggaaagca aaacaaagaa
    10261 gagcagctga atcaagtaac tttgtgggtg tttttgaatt atctgactcg agataaagag
    10321 cttcgttaaa aacggaatct tttttaaggt cgtatgcttt gtttgtctct ccggagattc
    10381 cgcgtgtgaa aagtcgtggt ttttgatctc tttgagatac ttaaaatttc cgagaaattg
    10441 gcggctctgt cggagataaa aagctgagat cttggagttc cccatttctg attaacgatt
    10501 ctctctgctg ttacttgagc taggaagcta ttaacaactt gagtgaaagt ttagggcttt
    10561 tgatgaagct ctcacgcaaa gagcttagct ttgtataagc tcagattcag attatttatt
    10621 gggtttattc ttcagagaaa gtaaagttga gtgatcaaca gtattcagtc gactggtacc
    10681 aacaggctcc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
    10741 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
    10801 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
    10861 ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag tgcttcagcc gctaccccga
    10921 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
    10981 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
    11041 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
    11101 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
    11161 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
    11221 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
    11281 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
    11341 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actcacggca tggacgagct
    11401 gtacaagatt gctgcagcgg ccgaattcaa gcgtgaagag caagcaagga aagctaaggt
    11461 gaacaatgag aaaaagacgg aaatagtgaa accagagagt tgtagcaatg aaggagatgt
    11521 caaggatctg aaaagaaagg actctgagga tggaaacgag ggtgaggaag aagaagcttc
    11581 ttcgaaaccg aaaaagccaa aagttgctct ttctcatctt caggacattg acgacacaga
    11641 agctgatcaa gaagaagagt caggtggatc ctagataacc tttaccttca ttccctgtaa
    11701 attggtaccc tgcaaagctt tcgttcgtat catcggtttc gacaacgttc gtcaagttca
    11761 atgcatcagt ttcattgcgc acacaccaga atcctactga gtttgagtat tatggcattg
    11821 ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg
    11881 ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc
    11941 cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat
    12001 ttgaaattat aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc
    12061 ctctaatgac cgaagttaat atgaggagta aaacacttgt agttgtacca ttatgcttat
    12121 tcactaggca acaaatatat tttcagacct agaaaagctg caaatgttac tgaatacaag
    12181 tatgtcctct tgtgttttag acatttatga actttccttt atgtaatttt ccagaatcct
    12241 tgtcagattc taatcattgc tttataatta tagttatact catggatttg tagttgagta
    12301 tgaaaatatt ttttaatgca ttttatgact tgccaattga ttgacaacat gcatcaatcg
    12361 actagtcgac gagtcagtaa taaacggcgt caaagtggtt gcagccggca cacacgagtc
    12421 gtgtttatca actcaaagca caaatacttt tcctcaacct aaaaataagg caattagcca
    12481 aaaacaactt tgcgtgtaaa caacgctcaa tacacgtgtc attttattat tagctattgc
    12541 ttcaccgcct tagctttctc gtgacctagt cgtcctcgtc ttttcttctt cttcttctat
    12601 aaaacaatac ccaaagagct cttcttcttc acaattcaga tttcaatttc tcaaaatctt
    12661 aaaaactttc tctcaattct ctctaccgtg atcaaggtaa atttctgtgt tccttattct
    12721 ctcaaaatct tcgattttgt tttcgttcga tcccaatttc gtatatgttc tttggtttag
    12781 attctgttaa tcttagatcg aagacgattt tctgggtttg atcgttagat atcatcttaa
    12841 ttctcgatta gggtttcata gatatcatcc gatttgttca aataatttga gttttgtcga
    12901 ataattactc ttcgatttgt gatttctatc tagatctggt gttagtttct agtttgtgcg
    12961 atcgaatttg tagattaatc tgagtttttc tgattaacac cacaccaatc tggtaccaga
    13021 aaaggggaga agcaaaacca tgggtaaaaa gcctgaactc accgcgacgt ctgtcgagaa
    13081 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
    13141 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
    13201 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
    13261 gattccggaa gtgcttgaca ttggggcatt cagcgagagc ctgacctatt gcatctcccg
    13321 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
    13381 accggtcgcg gaggcgatgg atgccatcgc tgcggccgat cttagccaga cgagcgggtt
    13441 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
    13501 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
    13561 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
    13621 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
    13681 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
    13741 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
    13801 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
    13861 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
    13921 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
    13981 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
    14041 cagcactcgt ccgagggcaa aggaatagat gcatcctgct ttaatgagat atgcgagacg
    14101 cctatgatcg catgatattt gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca
    14161 tgtgtagctc agatccttac cgccggtttc ggttcattct aatgaatata tcacccgtta
    14221 ctatcgtatt tttatgaata atattctccg ttcaatttac tgattgtacc ctactactta
    14281 tatgtacaat attaaaatga aaacaatata ttgtgctgaa taggtttata gcgacatcta
    14341 tgatagagcg ccacaataac aaacaattgc gttttattat tacaaatcca attttaaaaa
    14401 aagcggcaga accggtcaaa cctaaaagac tgattacata aatcttattc aaatttcaaa
    14461 agtgccccag gggctagtat ctacgacaca ccgagcggcg aactaataac gctcactgaa
    14521 gggaactccg gttccccgcc ggcgcgcatg ggtgagattc cttgaagttg agtattggcc
    14581 gtccgctcta ccgaaagtta cgggcaccat tcaacccggt ccagcacggc ggccgggtaa
    14641 ccgacttgct gccccgagaa ttatgcagca tttttttggt gtatgtgggc cccaaatgaa
    14701 gtgcaggtca aaccttgaca gtgacgacaa atcgttgggc gggtccaggg cgaattttgc
    14761 gacaacatgt cgaggctcag caggaccggc atgcaagcta gcttactagt gatattctat
    14821 agtgtcacct aaatgtat
//
