LOCUS       sequence-438493-       10570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stable expression of mouse IRAK4-mScarlet after Lentiviral 
            transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Fisch D, Zhang T, Sun H, Ma W, Tan Y, Gygi SP, Higgins DE, Kagan JC
  TITLE     Molecular definition of the endogenous Toll-like receptor signalling
            pathways.
  JOURNAL   Nature. 2024 Jul;631(8021):635-644. doi: 10.1038/s41586-024-07614-7.
            Epub 2024 Jul 3.
  PUBMED    38961291
REFERENCE   2  (bases 1 to 10570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-024-07614-7"; journalName: "Nature"; date: 
            "2024-07"; volume: "631"; issue: "8021"; pages: "635-644"
COMMENT     Sequence Label: pLenti-IRAK4-mScarlet-P2A-Puro
FEATURES             Location/Qualifiers
     source          1..10570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2055..2266
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             3664..4356
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(3794..3812)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4062..4081
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4375..4431
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4432..5028
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4925..4945
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5044..5632
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5097..5117)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5515..5526)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5704..5937
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5963..5980)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5969..6193
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6239..6667
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6326..6345)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6536..6557
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6676..6696)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6681..7010
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6861..6996
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6923..6942
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7058..7105
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7124..7498
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7628..7749
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7665..7684)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7719..7738
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7798..7814)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7798..7814)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7811..7833)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7822..7838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7846..7852,7853..7870,7871..7876))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7891..7912
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8029..8046)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8200..8788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8280..8299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8959..9750,9751..9819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9582..9601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9820..9924)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9999..10018)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10190..10569
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taaggtaccg ggagtatccg aattcgctag ctaggtcttg aaaggagtgg gaattggctc
     2041 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     2101 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     2161 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     2221 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctagac
     2281 gccaccatga acaagccgtt gacaccatcg acgtacatta gaaacctcaa tgttgggatt
     2341 ttgaggaagc tgagtgattt cattgaccct caggagggct ggaagaaact cgcggtggcc
     2401 atcaaaaagc cctctggtga cgaccgatac aatcagttcc atatcagaag attcgaagcc
     2461 cttctgcaaa cgggtaaatc cccgacgtgt gagcttttgt ttgactgggg cacaactaat
     2521 tgtacggtag gagatcttgt ggatctcctt gtccaaatcg aactgttcgc ccctgccacg
     2581 cttttgttgc ccgatgcagt tccacagaca gtgaaaagtc ttccacccag ggaggcggcg
     2641 actgtcgctc aaacacacgg tccgtgccaa gaaaaagacc gcaccagcgt catgcctatg
     2701 cccaagctcg aacattcatg tgagcctccc gattcttctt cccctgacaa ccgctcagta
     2761 gaatcatccg acactaggtt ccactcattt agttttcacg aacttaagag cattacgaat
     2821 aactttgatg agcagcccgc tagtgctggt ggcaatagaa tgggagaggg cggcttcgga
     2881 gtcgtttaca aaggatgcgt taataacacc atcgtcgcag tgaaaaagct gggtgctatg
     2941 gtggagataa gtacagagga gctcaagcag cagtttgacc aggagattaa ggtaatggca
     3001 acttgccaac acgaaaatct tgtagagctg ctgggcttta gttctgattc agacaacctg
     3061 tgtctggttt acgcgtacat gcccaatggt agtctccttg atcgcttgag ttgtctggat
     3121 ggaacacctc ccctgagctg gcacacccgg tgtaaagttg cccaagggac ggctaatgga
     3181 attcgcttcc tgcacgaaaa ccaccacata catagggaca tcaaatccgc gaatatcttg
     3241 ctggataagg acttcaccgc gaaaataagc gattttggcc ttgcacgcgc tagcgcaagg
     3301 ctggcccaga cagttatgac ttccaggatt gtaggaacaa cggcttatat ggcgcccgag
     3361 gccctgaggg gcgagatcac ccctaaaagc gacatatatt catttggcgt cgtattgttg
     3421 gagttgatta ctggactcgc tgccgttgat gagaacagag agccccaact tctgctggat
     3481 attaaggagg agatcgagga cgaagagaag accattgagg actatacaga tgaaaaaatg
     3541 agtgacgcgg atccggcgtc agttgaagca atgtactcag cggcctccca gtgcctccac
     3601 gaaaaaaaaa accgacggcc ggatattgcg aaggtgcaac agctgctaca agagatgtct
     3661 gctgtgagca agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag
     3721 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     3781 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac
     3841 atcctgtccc ctcagttcat gtacggctcc agggccttca ccaagcaccc cgccgacatc
     3901 cccgactact ataagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     3961 gaggacggcg gcgccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc
     4021 tacaaggtga agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaag
     4081 acaatgggct gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc
     4141 gacattaaga tggccctgcg cctgaaggac ggcggccgct acctggcgga cttcaagacc
     4201 acctacaagg ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg
     4261 gacatcacct cccacaacga ggactacacc gtggtggaac agtacgaacg ctccgagggc
     4321 cgccactcca ccggcggcat ggacgagctg tacaagtccc gcctcggatc cggcgcaaca
     4381 aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc gaccgagtac
     4441 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     4501 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     4561 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     4621 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     4681 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     4741 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     4801 ctggccaccg tcggagtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     4861 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     4921 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     4981 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac gcgttaagtc
     5041 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     5101 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     5161 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     5221 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     5281 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     5341 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     5401 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     5461 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     5521 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     5581 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     5641 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5701 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     5761 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5821 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5881 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     5941 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     6001 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     6061 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     6121 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     6181 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     6241 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6301 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6361 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6421 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6481 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6541 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6601 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6661 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6721 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6781 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6841 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6901 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     6961 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     7021 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     7081 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
     7141 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     7201 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     7261 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     7321 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     7381 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     7441 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     7501 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7561 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7621 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7681 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7741 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     7801 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7861 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7921 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7981 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8041 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8101 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8161 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8221 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8281 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8341 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8401 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8461 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8521 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8581 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8641 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8701 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8761 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8821 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8881 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8941 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9001 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9061 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9121 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9181 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9241 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9301 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9361 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9421 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9481 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9541 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9601 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9661 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9721 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9781 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9841 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9901 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     9961 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
    10021 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
    10081 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
    10141 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
    10201 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
    10261 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
    10321 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
    10381 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
    10441 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
    10501 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
    10561 attaccatgg
//
