LOCUS       sequence-438719-       14848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional reporter for ARF7 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14848)
  TITLE     Bishopp Lab ARF7 Reporter Plasmids 2024
REFERENCE   2  (bases 1 to 14848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pro::EX1-2(int-Delta-NAC2):GFP
FEATURES             Location/Qualifiers
     source          1..14848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(550..568)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(551..568)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    618..640
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     rep_origin      767..1202
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     CDS             1493..2281
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2452..3040
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2941..2960
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3131..3155
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(3251..3958)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    3977..4001
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5804..5859
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    complement(5881..5905)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(11273..11275,11276..11278,11279..11989)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(11318..11339)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11579..11598)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11926..11947
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      12298..12939
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             13622..14650
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      join(14658..14848,1..517)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 taccctacta cttatatgta caatattaaa atgaaaacaa tatattgtgc tgaataggtt
       61 tatagcgaca tctatgatag agcgccacaa taacaaacaa ttgcgtttta ttattacaaa
      121 tccaatttta aaaaaagcgg cagaaccggt caaacctaaa agactgatta cataaatctt
      181 attcaaattt caaaagtgcc ccaggggcta gtatctacga cacaccgagc ggcgaactaa
      241 taacgctcac tgaagggaac tccggttccc cgccggcgcg catgggtgag attccttgaa
      301 gttgagtatt ggccgtccgc tctaccgaaa gttacgggca ccattcaacc cggtccagca
      361 cggcggccgg gtaaccgact tgctgccccg agaattatgc agcatttttt tggtgtatgt
      421 gggccccaaa tgaagtgcag gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc
      481 agggcgaatt ttgcgacaac atgtcgaggc tcagcaggac cggcatgcaa gctagcttac
      541 tagtgatatt ctatagtgtc acctaaatgt attctagacc aagcatgcac agctggcgtt
      601 ctcagccaga tccaacgtta caccacaata tatcctgcca agatctaatt ccggggatcg
      661 gaaatccaga agcccgagag gttgccgcct ttcgggcttt ttctttttca aaaaaaaaaa
      721 tttataaaac gatctgttgc ggccggccgc cgggttgtgg gcaaaggcgc tcgacggtgg
      781 gcaaccgctt gcggttgtcc acgggcggag ccggtgcgcg tagcgcattg tccacaagcc
      841 aagggcgacc aataattgat atatatattc ataattgaaa agctaattga acatactact
      901 tgctgtaact acttgccgga gcgaggggtg tttgcaagct gttgatctga aagggctatt
      961 agcgttctca cgtgcctttt tgattagcga tttcacgtga ccttattagc gatttcacgt
     1021 actccgatta gcgatttcac gtaccctgat tagcgatttc acgtggatag tttttggagc
     1081 gggccggaaa gccccgtgaa tcaaggcttt gcggggcatt agcggtttca cgtggataac
     1141 taccctctat ccacaggctt ccggggataa aaaagcccgc tcgacggcgg gctgttggat
     1201 ggggatcgcc tgaatcgccc catcatccag ccagaaagtg agggagccac ggttgatgag
     1261 agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca cggaacggtc
     1321 tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc gatttattca
     1381 acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     1441 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgaggga
     1501 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     1561 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     1621 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     1681 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     1741 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     1801 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     1861 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     1921 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     1981 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     2041 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     2101 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     2161 catacttgaa gctaggcagg cttatcttgg acaagaagat cgcttggcct cgcgcgcaga
     2221 tcagttggaa gaatttgttc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     2281 atcactagac caatgttaca catatatact ttagattgat ttaaaacttc atttttaatt
     2341 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2401 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2461 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2521 ttgtttgccg gatcaagagc taccaactct tcttccgaag gtaactggct tcagcagagc
     2581 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2641 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2701 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2761 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2821 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2881 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2941 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3001 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3061 tttacggttc ctggcctttt gctggccttt tgctcacatg agatctcaaa caaacacata
     3121 cagcgactta gtttacccgc caatatatcc tgtcaaggct cgaactaggc tcggacgaag
     3181 taagcggccg caaccttaat taaacacggc gcgccaacag cgatcgcacc aggtaccttg
     3241 catgccggtc ctgctgagcc tcgacatgtt gtcgcaaaat tcgccctgga cccgcccaac
     3301 gatttgtcgt cactgtcaag gtttgacctg cacttcattt ggggcccaca tacaccaaaa
     3361 aaatgctgca taattctcgg ggcagcaagt cggttacccg gccgccgtgc tggaccgggt
     3421 tgaatggtgc ccgtaacttt cggtagagcg gacggccaat actcaacttc aaggaatctc
     3481 acccatgcgc gccggcgggg aaccggagtt cccttcagtg agcgttatta gttcgccgct
     3541 cggtgtgtcg tagatactag cccctggggc acttttgaaa tttgaataag atttatgtaa
     3601 tcagtctttt aggtttgacc ggttctgccg ctttttttaa aattggattt gtaataataa
     3661 aacgcaattg tttgttattg tggcgctcta tcatagatgt cgctataaac ctattcagca
     3721 caatatattg ttttcatttt aatattgtac atataagtag tagggtacaa tcagtaaatt
     3781 gaacggagaa tattattcat aaaaatacga tagtaacggg tgatatattc attagaatga
     3841 accgaaaccg gcggtaagga tctgagctac acatgctcag gttttttaca acgtgcacaa
     3901 cagaattgaa agcaaatatc atgcgatcat aggcgtctcg catatctcat taaagcagga
     3961 ctctagagga tcatcaacca ctttgtacaa gaaagctggg tcggcgcgcc caccctttta
     4021 cttgtacaac tcgtccatac cgtagaggaa gaggtggtgg cggccctcgg aacgctcgta
     4081 ctgttcgaca atggtgtaat cctcgttgtg ggaggtaatg tccaacttgg tatcaacgta
     4141 gtaataacca ggcaattgaa cgggtttctt ggccatgtag atggtcttaa actccacgag
     4201 gtaatgtcca ccgtccttca acttcaatgc ctggtgaatc tcgcccttca acactccatc
     4261 gcggggatac aagcgctctg ttgaagcctc ccagcccata gtcttcttct gcataactgg
     4321 gccgtctggt gggaaattgg ttccacgcat tttgaccttg tagataagcg taccatcttg
     4381 aagggaggag tcttgggtga ctgtaaccaa accaccgtcc tcgaagttca taacgcgctc
     4441 ccacttaaat ccttctggga aagacaactt cttataatct ggaatatcgg ctgggtgttt
     4501 cacgtaagcc ttggaaccgt acataaactg tggtgagaga atatcccaag cgaaaggaag
     4561 tggtccaccc ttggtgactt tcaacttagc tgtctgagtg ccctcatatg ggcggccctc
     4621 accctctcct tcgatctcga actcgtggcc attcattgag ccctccatac ggactttgaa
     4681 gcacataaac tccttgatca cagccatgtt attatcctcg gatgaagcgg tgccagagct
     4741 gccactaccg gtgcttccag taccatgacc caagaaaagg tgatggcgac cctcagacct
     4801 ctcatattgt tcaacgatgg tgtagtcctc gttgtgggag gtgatatcga gtttagtgtc
     4861 cacgtagtaa tagccgggga gttgcacggg cttcttagcc atgtagatgg tcttgaactc
     4921 caccaagtag tggcctccat ccttaagttt gagagcttgg tggatttcac ccttaagaac
     4981 gccgtctctt gggtaaagcc tctcagtgga ggcttcccaa cccatagttt tcttttgcat
     5041 gactggacca tcgggaggaa agtttgtgcc tctcatcttc accttgtaga tcaaagtgcc
     5101 gtcctgcaaa ctagaatctt gggtcacagt cacaagacct ccatcctcga agttcatgac
     5161 cctttcccac ttgaaaccct ctgggaagga caactttttg taatcgggga tgtcagcagg
     5221 atgcttaacg tatgccttag agccatacat gaattggggg gacaagatgt cccaagcgaa
     5281 gggcaaagga ccacccttag tcactttgag ctttgcggtt tgggtgccct cgtagggtct
     5341 accttcaccc tcgccctcga tctcgaactc gtgaccgttc atggaacctt ccatcctaac
     5401 cttgaatctc atgaactctt tgataacctc ctcacccttg gaaaccatgg atcgggctgc
     5461 cgcagcggca gcagccgcag caggtttggt gaggacataa atggcgtata tgatcccagg
     5521 aatataccca agtagcgtca aaaccaaaca tatccaaaac tcaacctgca acaaaattca
     5581 taatttcaac ttttaatttc tagtgtggat aaatgtaaaa aaagaaccat atttgactaa
     5641 agtataagat taattaacca aaataaaact cagtctaaat atgagaggaa agataaatac
     5701 cccgcaacca aatctgagaa agacaccgag tggaggcaag aggatggcga taataatatc
     5761 aacgaaagta gctgtactca ttttggtgaa gggggcggcc gctgtaattg taaatagtaa
     5821 ttgtaatgtt gtttgttgtt tgttgttgtt ggtaattgtt gtaaaaatac gcggccgcgg
     5881 agcctgcttt tttgtacaaa cttgttgatg atccctgtta atcagaaaaa ctcagattaa
     5941 tcgacaaatt cgatcgcaca aactagaaac taacaccaga tctagataga aatcacaaat
     6001 cgaagagtaa ttattcgaca aaactcaaat tatttgaaca aatcggatga tatctatgaa
     6061 accctaatcg agaattaaga tgatatctaa cgatcaaacc cagaaaatcg tcttcgatct
     6121 aagattaaca gaatctaaac caaagaacat atacgaaatt gggatcgaac gaaaacaaaa
     6181 tcgaagattt tgagagaata aggaacacag aaatttacct tgatcacggt agagagaatt
     6241 gagagaaagt ttttaagatt ttgagaaatt gaaatctgaa ttgtgaagaa gaagagctct
     6301 ttgggtattg ttttatagaa gaagaagaag aaaagacgag gacgactagg tcacgagaaa
     6361 gctaaggcgg tgaagcaata gctaataata aaatgacacg tgtattgagc gttgtttaca
     6421 cgcaaagttg tttttggcta attgccttat ttttaggttg aggaaaagta tttgtgcttt
     6481 gagttgataa acacgactcg tgtgtgccgg ctgcaaccac tttgacgccg tttattactg
     6541 actcgtcgac aaccacaatt tctaacggtc gtcataagat ccagccgttg agatttaacg
     6601 atcgttacga tttatatttt tttagcatta tcgttttatt ttttaaatat acggtggagc
     6661 tgaaaattgg caataattga accgtgggtc ccactgcatt gaagcgtatt tcgtattttc
     6721 tagaattctt cgtgctttat ttcttttcct ttttgttttt ttttgccatt tatctaatgc
     6781 aagtgggctt ataaaatcag tgaatttctt ggaaaagtaa cttctttatc gtataacata
     6841 ttgtgaaatt atccatttct tttaattttt tagtgttatt ggatattttt gtatgattat
     6901 tgatttgcat aggataatga cttttgtatc aagttggtga acaagtctcg ttaaaaaagg
     6961 caagtggttt ggtgactcga tttattcttg ttatttaatt catatatcaa tggatcttat
     7021 ttggggcctg gtccatattt aacactcgtg ttcagtccaa tgaccaataa tattttttca
     7081 ttaataacaa tgtaacaaga atgatacaca aaacattctt tgaataagtt cgctatgaag
     7141 aagggaactt atccggtcct agatcatcag ttcatacaaa cctccataga gttcaacatc
     7201 ttaaacaaga atatcctgat ctgaagaatg tggaggcttt agtcccttgg atacttggga
     7261 ggctgtggaa gaacagaaac gagctggtgc ttaaagggag ggaatttgga accaatgagg
     7321 tattagtaag gacacaagaa gatgcagatg agtggattag aaggaaagag gctcagaatg
     7381 taaggaaagc accaaccaca acaccgtcgg gacagacgag aaacatggga agctccacca
     7441 caatcttggg ttaagtgcaa ctttgatggg gcatggccaa cagaaggatt aaaatgtggc
     7501 ttagggtggg tgcttcgcga tcatacaggg aaggtgttat ggttaggtgc aagagctgtg
     7561 gtaaaagtaa gaagcgtgct ggaagtagaa gtggaggctc ttagatgggc tgtgctgtca
     7621 ttatcccgat tcaattatag gaagatcatt tttgaggtgg attctcagca acttgtgtct
     7681 ttggttacat gaaagttatg cttgtcaagt ctcaatccaa ttatccaaga cataaagtat
     7741 ctacttagca agtttgagga ttttatgctt gtgcatacaa gccgagaagg aaatggagtg
     7801 gcagatagaa tagctaagga atctctttct tttgagaatt atgatctaaa gttgtattct
     7861 attgtaccaa tttgggttaa aagctctgtt gagctagact catatgcata cctaagagat
     7921 gtcgcaaacc agctcatgtc accaaacaac aattagagcc aatgtgaaat ggggtgggag
     7981 tgtgaccacg tcaggtggga tttgccgtga gaggcccttc tttattgttt gttccttttt
     8041 cttcaattat tattttcaat aataatgtaa ttaaacttat catcaaatac aaatattcga
     8101 taaattaaag gatcctctac aagaaatatg aatattgata acacataaga atagcaaaat
     8161 ttttgtaact attattaaaa ataatattcg agatttgtta accatagtat aacatttgta
     8221 aaccactaaa cataaaagta acgagaaaat ctcctaaagc aaagtaacgt ttctctatat
     8281 ttagcgtttt acaaaattta tgttaaaaaa ttatgtttag cgttttacaa aaattatgct
     8341 agaaaagttt atgtttagcg gaaatctaaa atcacttaaa acgcaaaaaa ttaatcagta
     8401 cgtagattat tttccacaac tctctcttac atacaaatta acaaataatg catatcagaa
     8461 tgacatagta aagtcgagaa aatcatggaa tcgtaacgaa acttcctcgt ctctctctga
     8521 taattcatcc aactcaagtg gtgtattttg tggttacatt tacatatatt gtcaacacca
     8581 actgtcgaat tcattaaatc ggctaaaata cgttgatgtg attctggaaa tatctttagg
     8641 acagtatatg agttattcat cttgttcatt ttccactttc cactgcggac accatacgga
     8701 cacacaaccg gttctgaaat gcgtcacgct cttcttcctt tcattccaac tttaatacat
     8761 gcatgaagtc gtagttcgga gttcatacta aatttcggtc actttgtaat aagaaaacta
     8821 ttaacatttt tctttttctt tttttaaacg ttagctaggc ctcaaagttt cacaaaaaaa
     8881 aaagaaaaaa aaaagctagg cgtcgaaatc cataacttca aaaggccttc ttacattcat
     8941 ccaatctcca aacttctgtc ttcacgcatt tattgctcag ggacatcatg tcgctactca
     9001 ctgccactta tttgaattga catccattca ttccatacat atatattacc ttcacttaaa
     9061 gttgtaagta tatagaattt taagcattcc tgccaaataa gttttggatc gaacctaaac
     9121 taacaaagag gttactgttt tgtaactttc ttttttgatg tggggtcatt caaagggttt
     9181 acatgcattt tacaatggtc tgtgatgaat atggaggttc agtctttggt agataaaact
     9241 tgttatgtgg ccttaaacca aatagattgt agtttgtaca atgacatatt tatggttata
     9301 ttcggttttc caatatgttt aggaatatat atcaccgttt ttttttgggt agacatttta
     9361 acgattatag aggaaaattt tgaatgatgg ctgtactggg aaaagagtgt aagttgtgat
     9421 catcatatgt aattttattt tgtgtgacat tataacactt cttccaaaag acaaaagttt
     9481 aggttaatta atataagaaa ccaataaaga atcatgaaca aagagagaac ttctatcatt
     9541 tttgatatga aatcgtccgt taaaatattg ttctttgtga tcgcatatgc tgtatgtact
     9601 tagatttgat caattaatca acatttacaa caaaataaga tcaattgttt ataatgatga
     9661 tctaagcgaa tttagatttg tttgttatta tagaactcat tactaaacat ttaagttcca
     9721 ttctcgttta aaacatgaac gctagtttat gaaactcaga accaaccatg taaaactcca
     9781 caaaatttct agtaaaatgc ttttgttcaa aataatgaaa atcataaaat tttttgatga
     9841 attatgaatc ctaaaatcta tctgaattaa atatagttaa ctacaaaaga aattgccaaa
     9901 catatatgta cgcactttga tcaaataact agctagtcag cttagtttgt ttttttcaat
     9961 acatccccta taaatctatg ttatccctaa tcaagtatta caataaatga tacagtaata
    10021 ataaccttaa tgaaaaaaac ttatcaaatt atcagagata aggaaaaaga aaacactaat
    10081 aaagttgttt ttttgtgtgt caaagttaaa attttagagg acaataataa cattaagaaa
    10141 ttaaatcgtg gccagtttgc tagtgaatat gtcggcttca tgagcataac taaataggta
    10201 taaaaaaata aaaatatcgg aaaaagaaaa gagaaggaaa aaaaaaggag aggaagaaga
    10261 aaaagctgtt cctgtgcttt agcagacctt ttggtagttg tcctggtcgc tgatcatccc
    10321 tttccgacaa ctctgttgct ctttctctca cgtttgagtc ttaatcttcg attgtccttg
    10381 gtcatcctta agttttgttt ttctctttcg attcattttc cagatttctt tttatagaaa
    10441 cccgtctctt tgatttgatt ccccccaaga aaggaaagca aaacaaagaa gagcagctga
    10501 atcaagtaac tttgtgggtg tttttgaatt atctgactcg agataaagag cttcgttaaa
    10561 aacggaatct tttttaaggt cgtatgcttt gtttgtctct ccggagattc cgcgtgtgaa
    10621 aagtcgtggt ttttgatctc tttgagatac ttaaaatttc cgagaaattg gcggctctgt
    10681 cggagataaa aagctgagat cttggagttc cccatttctg attaacgatt ctctctgctg
    10741 ttacttgagc taggaagcta ttaacaactt gagtgaaagt ttagggcttt tgatgaagct
    10801 ctcacgcaaa gagcttagct ttgtataagc tcagattcag attatttatt gggtttattc
    10861 ttcagagaaa gtaaagttga gtgatcaaca ccatgaaagc tccttcatca aatggagttt
    10921 ctcctaatcc tgttgaaggt ttgtgtgttt ctcgtatctg ataaatgtca atcctttagt
    10981 agaagaaatt cattgctgga tttgaataga ttcaagtttt aagtagaaga ggtcacacat
    11041 ttcttcagaa ttgctggatt tgagaacctg aattgaattg gtttcggtca ttttctgtct
    11101 agagagtaag ctaagttact attgtattgg tttataaaga ctgtggcttt tagttggttt
    11161 agagcagttc tctcctatct tgttggtttg ataatagtaa ctgaatgaag atcctagaga
    11221 ttttaagtaa tcacagcttt gatgttgtgt gaatgcagga gaaaggggct ccatggtgag
    11281 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
    11341 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
    11401 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
    11461 caccttcacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
    11521 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
    11581 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
    11641 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
    11701 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
    11761 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
    11821 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
    11881 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
    11941 gttcgtgacc gccgccggga tcactcacgg catggacgag ctgtacaaga ttgctgcagc
    12001 ggccgaattc aagcgtgaag agcaagcaag gaaagctaag gtgaacaatg agaaaaagac
    12061 ggaaatagtg aaaccagaga gttgtagcaa tgaaggagat gtcaaggatc tgaaaagaaa
    12121 ggactctgag gatggaaacg agggtgagga agaagaagct tcttcgaaac cgaaaaagcc
    12181 aaaagttgct ctttctcatc ttcaggacat tgacgacaca gaagctgatc aagaagaaga
    12241 gtcaggtgga tcctagataa cctttacctt cattccctgt aaattggtac cctgcaaagc
    12301 tttcgttcgt atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca gtttcattgc
    12361 gcacacacca gaatcctact gagtttgagt attatggcat tgggaaaact gtttttcttg
    12421 taccatttgt tgtgcttgta atttactgtg ttttttattc ggttttcgct atcgaactgt
    12481 gaaatggaaa tggatggaga agagttaatg aatgatatgg tccttttgtt cattctcaaa
    12541 ttaatattat ttgttttttc tcttatttgt tgtgtgttga atttgaaatt ataagagata
    12601 tgcaaacatt ttgttttgag taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta
    12661 atatgaggag taaaacactt gtagttgtac cattatgctt attcactagg caacaaatat
    12721 attttcagac ctagaaaagc tgcaaatgtt actgaataca agtatgtcct cttgtgtttt
    12781 agacatttat gaactttcct ttatgtaatt ttccagaatc cttgtcagat tctaatcatt
    12841 gctttataat tatagttata ctcatggatt tgtagttgag tatgaaaata ttttttaatg
    12901 cattttatga cttgccaatt gattgacaac atgcatcaat cgactagtcg acgagtcagt
    12961 aataaacggc gtcaaagtgg ttgcagccgg cacacacgag tcgtgtttat caactcaaag
    13021 cacaaatact tttcctcaac ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta
    13081 aacaacgctc aatacacgtg tcattttatt attagctatt gcttcaccgc cttagctttc
    13141 tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct ataaaacaat acccaaagag
    13201 ctcttcttct tcacaattca gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt
    13261 ctctctaccg tgatcaaggt aaatttctgt gttccttatt ctctcaaaat cttcgatttt
    13321 gttttcgttc gatcccaatt tcgtatatgt tctttggttt agattctgtt aatcttagat
    13381 cgaagacgat tttctgggtt tgatcgttag atatcatctt aattctcgat tagggtttca
    13441 tagatatcat ccgatttgtt caaataattt gagttttgtc gaataattac tcttcgattt
    13501 gtgatttcta tctagatctg gtgttagttt ctagtttgtg cgatcgaatt tgtagattaa
    13561 tctgagtttt tctgattaac accacaccaa tctggtacca gaaaagggga gaagcaaaac
    13621 catgggtaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
    13681 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
    13741 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
    13801 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
    13861 cattggggca ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
    13921 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg caaccggtcg cggaggcgat
    13981 ggatgccatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
    14041 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
    14101 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
    14161 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
    14221 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
    14281 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
    14341 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
    14401 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
    14461 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
    14521 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
    14581 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
    14641 aaaggaatag atgcatcctg ctttaatgag atatgcgaga cgcctatgat cgcatgatat
    14701 ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc tcagatcctt
    14761 accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta tttttatgaa
    14821 taatattctc cgttcaattt actgattg
//
