LOCUS       sequence-438762-        9376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression Plasmid of anti-Neurabin-1/Ppp1r9a (Mouse) 
            IgG2a R-mAb. Derived from hybridoma L129/96..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9376)
  AUTHORS   Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, 
            Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles 
            ML, Froenicke L, Trimmer JS
  TITLE     High-volume hybridoma sequencing on the NeuroMabSeq platform enables
            efficient generation of recombinant monoclonal antibodies and scFvs 
            for neuroscience research.
  JOURNAL   Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4.
  PUBMED    37758930
REFERENCE   2  (bases 1 to 9376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-09-27"; volume: "13(1):16200. doi"; pages: " 
            10.1038/s41598-023-43233-4"
COMMENT     Sequence Label: Anti-Neurabin-1/Ppp1r9a [L129/96R]
FEATURES             Location/Qualifiers
     source          1..9376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        271..650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        651..854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      1398..1407
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     enhancer        2393..2696
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2697..2900
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4127..4822
                     /codon_start=1
                     /product="CH2 and CH3 domains of the mouse IgG2a heavy 
                     chain plus the hinge region"
                     /label=Fc tag
                     /translation="PRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVT
                     CVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEF
                     KCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIY
                     VEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHT
                     TKSFSRTPGK"
     polyA_signal    4873..4928
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4927..4946)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7164..7182)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7250..7354
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7355..7423,7424..8215)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7573..7592)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8386..8974
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8875..8894
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9128..9145
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9262..9283
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9298..9303,9304..9321,9322..9328)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9336..9352
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9341..9363
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9360..9376
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9360..9376
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgattacg ccaagctcta gctagaggtc gaccaattct catgtttgac agcttatcat
       61 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
      121 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
      181 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
      241 ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt
      301 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      361 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      421 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      481 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
      541 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
      601 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      661 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      721 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      781 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      841 atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc atcgctgtct
      901 gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct ttccagtact
      961 cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga cctgagcgag
     1021 tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa
     1081 ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt tctggcggag
     1141 gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt cgaggtgagg
     1201 tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag cgggcattac
     1261 ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccga
     1321 tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca caggtgtcca
     1381 ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg tccctgctgc
     1441 tctgcatgtc tggtgcggcc gcagatgttg tgatgaccca gactccgctc actttgtcgg
     1501 ttaccattgg acaaccagcc tccatctctt gcaaatcaag tcagagcctc ttagatagtg
     1561 atggaaagac atatttgaat tggttgttac agagaccagg ccagtctcca aagcgcctaa
     1621 tctatctggt gtctacactg gactctggag tccctgacag gttcactggc agtggatcag
     1681 ggacagattt cacactgaaa atcagcagag tggaggctga ggatttggga atttattatt
     1741 gctggcaagg tacacatttt ccgtggacgt tcggtggagg caccaagctg gaaatcaaac
     1801 gggctgatgc tgcaccaact gtatccatct tcccaccatc cagtgagcag ttaacatctg
     1861 gaggtgcctc agtcgtgtgc ttcttgaaca acttctaccc caaagacatc aatgtcaagt
     1921 ggaagattga tggcagtgaa cgacaaaatg gcgtcctgaa cagttggact gatcaggaca
     1981 gcaaagacag cacctacagc atgagcagca ccctcacgtt gaccaaggac gagtatgaac
     2041 gacataacag ctatacctgt gaggccactc acaagacatc aacttcaccc attgtcaaga
     2101 gcttcaacag gaatgagtgt tagagacaaa ggtcctgaga cgccaccacc agctccccag
     2161 ctccatccta tcttcccttc taaggtcttg gaggcttccc cacgagcgac ctaccactgt
     2221 tgcggtgctc caaacctcct ccccacctcc ttctcctcct cctccctttc cttggctttt
     2281 atcatgctaa tatttgcaga aaatattcaa taaagtgagt ctttgcactt gatagttatt
     2341 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     2401 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     2461 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     2521 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc
     2581 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     2641 tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     2701 tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     2761 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     2821 caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg
     2881 aggtctatat aagcagagct cgtttagtga accgtcagat cctcactctc ttccgcatcg
     2941 ctgtctgcga gggccagctg ttgggctcgc ggttgaggac aaactcttcg cggtctttcc
     3001 agtactctcg gatcggaaac ccgtcggcct ccgaacggta ctccgccacc gagggacctg
     3061 agcgagtccg catcgaccgg atcggaaaac ctctcgagaa aggcgtctaa ccagtcacag
     3121 tcgcaaggta ggctgagcac cgtggcgggc ggcagcgggt ggcggtcggg gttgtttctg
     3181 gcggaggtgc tgctgatgat gtaattaaag taggcggtct tgagacggcg gatggtcgag
     3241 gtgaggtgtg gcaggcttga gatccagctg ttggggtgag tactccctct caaaagcggg
     3301 cattacttct gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcaccta
     3361 gcccgatctg gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg
     3421 tgtccactcc caggccacca tgaagctgcc tgttctgcta gtggtgctgc tattgttcac
     3481 gagtccagcc tcaagcagtg aggtccagct gcagcagtct ggacctgagc tggtgaagcc
     3541 cggggcttca gtgaagatat cctgtaagac ttctggatac acattcactg attacaccat
     3601 gaactgggtg aaacagagcc atggaaagag ccttgagtgg attggagtta ttagtccaag
     3661 cgatggtgtt accacctaca accagatgtt caagggcaag gccactttga ctgtagacaa
     3721 gtcctccagc acagcctaca tggaactccg cagactgaca tctgaggatt ctgcagtcta
     3781 ttactgtgca ttttctttct ggggccaagg gactctggtc actgtctctg cagcgcgccc
     3841 aacagcccca tcggtctatc cactggcccc tgtgtgtgga gatacaactg gctcctcggt
     3901 gactctagga tgcctggtca agggttattt ccctgagcca gtgaccttga cctggaactc
     3961 tggatccctg tccagtggtg tgcacacctt cccagctgtc ctgcagtctg acctctacac
     4021 cctcagcagc tcagtgactg taacctcgag cacctggccc agccagtcca tcacctgcaa
     4081 tgtggcccac ccggcaagca gcaccaaggt ggacaagaaa attgagccca gagggcccac
     4141 aatcaagccc tgtcctccat gcaaatgccc agcacctaac ctcttgggtg gaccatccgt
     4201 cttcatcttc cctccaaaga tcaaggatgt actcatgatc tccctgagcc ccatagtcac
     4261 atgtgtggtg gtggatgtga gcgaggatga cccagatgtc cagatcagct ggtttgtgaa
     4321 caacgtggaa gtacacacag ctcagacaca aacccataga gaggattaca acagtactct
     4381 ccgggtggtc agtgccctcc ccatccagca ccaggactgg atgagtggca aggagttcaa
     4441 atgcaaggtc aacaacaaag acctcccagc gcccatcgag agaaccatct caaaacccaa
     4501 agggtcagta agagctccac aggtatatgt cttgcctcca ccagaagaag agatgactaa
     4561 gaaacaggtc actctgacct gcatggtcac agacttcatg cctgaagaca tttacgtgga
     4621 gtggaccaac aacgggaaaa cagagctaaa ctacaagaac actgaaccag tcctggactc
     4681 tgatggttct tacttcatgt acagcaagct gagagtggaa aagaagaact gggtggaaag
     4741 aaatagctac tcctgttcag tggtccacga gggtctgcac aatcaccaca cgactaagag
     4801 cttctcccgg actccgggta aatgatctag acacgtgatt aattaaggat cccccgacct
     4861 cgacctctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     4921 gtctctcact cggaaggaca tatgggaggg caaatcattt ggtcgagatc cctcggagat
     4981 ctctagctag aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc
     5041 cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg
     5101 ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag
     5161 ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag
     5221 cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga
     5281 agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag
     5341 gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg
     5401 gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc
     5461 ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg
     5521 ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc
     5581 caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa
     5641 ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa
     5701 tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa
     5761 cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa
     5821 tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc
     5881 ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa
     5941 tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta
     6001 tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca
     6061 gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg
     6121 gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc
     6181 tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg
     6241 agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg
     6301 tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa
     6361 aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg
     6421 cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag
     6481 ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg
     6541 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     6601 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     6661 tatatgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag
     6721 catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag
     6781 catatgctat cctaatagag attagggtag tatatgctat cctaatttat atctgggtag
     6841 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     6901 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     6961 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     7021 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     7081 tgctatccta atctgtatcc gggtagcata tgctatcctc atgataagct gtcaaacatg
     7141 agaattttct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     7201 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7261 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7321 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7381 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7441 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7501 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7561 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     7621 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7681 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7741 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7801 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7861 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     7921 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7981 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8041 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8101 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8161 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8221 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8281 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8341 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8401 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8461 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8521 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8581 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8641 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8701 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8761 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8821 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8881 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8941 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9001 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9061 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9121 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9181 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     9241 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     9301 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     9361 aggaaacagc tatgac
//
