LOCUS       sequence-438791-        9503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human IFIT5_K206R in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9503)
  AUTHORS   Broderick K, Moutaoufik MT, Saccon T, Malty R, Amin S, Phanse S, 
            Joseph TP, Zilocchi M, Hosseinnia A, Istace Z, Hajikarimlou M, Abrar
            S, Fisher J, Brassard R, Perera R, Kumar A, Aoki H, Rahmatbakhsh M, 
            Jessulat M, Kobasa D, Dehne F, Prasad B, Gagarinova A, Joanne 
            Lemieux M, Cochrane A, Houry WA, Aly KA, Golshani A, Babu M
  TITLE     Human protein interaction networks of ancestral and variant 
            SARS-CoV-2 in organ-specific cells and bodily fluids.
  JOURNAL   Nat Commun. 2025 Jul 1;16(1):5784. doi: 10.1038/s41467-025-60949-1.
  PUBMED    40593736
REFERENCE   2  (bases 1 to 9503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-1"; volume: "16(1):5784. doi"; pages: " 
            10.1038/s41467-025-60949-1"
COMMENT     Sequence Label: pLD-puro-Cc-IFIT5_K206R-VA
FEATURES             Location/Qualifiers
     source          1..9503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             419..463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     761..781
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     enhancer        1009..1312
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1313..1516
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1467..1487
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1513..1537
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1574..1639
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1649..1669
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1682..1702
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1715..1732
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1745..1768
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1805..1828
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    1910..1934
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3432..3456)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3525..3642
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3691..4201
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4120..4138
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4223..4822
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4223..4242)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4719..4739
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4950..5183
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5255..5376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5292..5311)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5346..5365
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5416..5551
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5571..5590)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5572..5590)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5600..5617)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5600..5616)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5609..5631)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5758..6213
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5845..5864)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6055..6076
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6239..6343
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6344..6412,6413..7204)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6562..6581)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7375..7963
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7864..7883
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8117..8134
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8251..8272
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8287..8292,8293..8310,8311..8317)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8325..8341
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8330..8352
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8349..8365
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8349..8365
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8384..8404
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8386..8404
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8432..8658
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8659..8839
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8886..9011
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
       61 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      301 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      421 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      481 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      541 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      601 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      661 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
      721 tcgatcacga gactagcctc gagcggccgc ccccttcacc gagggcctat ttcccatgat
      781 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
      841 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      901 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgttgacta gttattaata
      961 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     1021 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     1081 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     1141 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     1201 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     1261 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     1321 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     1381 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     1441 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     1501 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
     1561 tgctagcacc atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta
     1621 caaggatgac gatgacaagc ctgcaggaga aaatctgtac ttccagggga gaggaggcag
     1681 cgagaacctg tactttcagg ggcctgcagg aagacatcac catcaccatc acagcggcgg
     1741 aagatggagc caccctcagt tcgagaaggg aggaggaagc ggcggaggca gcggaggagg
     1801 aagctggagc cacccgcagt tcgagaaagc gatcgccaga gagctgttca acctgctggg
     1861 cgagaaccag ccccccgtgg tgatcaaggc gatcgccacc ggtaagctta caagtttgta
     1921 caaaaaagtt ggcatgagtg aaattcgtaa ggacaccttg aaggccattc tgttggagtt
     1981 agaatgtcat tttacatgga atttacttaa ggaagacatt gatctgtttg aggtagaaga
     2041 tacaattggg caacagcttg aatttcttac cacaaaatct agacttgctc tttataacct
     2101 attggcctat gtgaaacacc taaaaggcca aaataaagac gcccttgagt gcttggaaca
     2161 agcagaagaa ataatccagc aagaacactc agacaaagaa gaagtacgaa gcctggtcac
     2221 ttggggaaac tatgcctggg tgtattatca catggaccag cttgaagaag ctcagaagta
     2281 tacaggtaag atagggaatg tctgtaagaa attgtccagt ccttctaact acaagttgga
     2341 gtgtcctgag actgactgtg agaaaggctg ggcactcttg aaatttggag gaaagtatta
     2401 tcaaaaggct aaagcggctt ttgagaaggc tctggaagtg gagcctgaca atccagaatt
     2461 taacatcggc tatgctatca cagtgtatcg gctggatgat tctgatagag aagggtctgt
     2521 aaagagcttt tctctggggc ctttgagacg ggctgttacc ctaaagtctg taaagagctt
     2581 ttctctgggg cctttgagac gggctgttac cctgaaccca gataacagct atattaaggt
     2641 ttttctggca ctgaagcttc aagatgtaca tgcagaagct gaaggggaaa agtatattga
     2701 agaaatcctg gaccaaatat catcccagcc ttacgtcctt cgttatgcag ccaagttcta
     2761 taggagaaaa aattcctgga acaaagctct cgaactttta aaaaaggcct tggaggtgac
     2821 accaacttct tctttcctgc atcaccagat gggactttgc tacagggcac aaatgatcca
     2881 aatcaagaag gccacacaca acagacctaa aggaaaggat aaactaaagg ttgatgagct
     2941 gatttcatct gctatatttc atttcaaagc agccatggaa cgagactcta tgtttgcatt
     3001 tgcctacaca gacctggcca acatgtatgc tgaaggaggc cagtatagca atgctgagga
     3061 cattttccgg aaagctcttc gtctggagaa cataaccgat gatcacaaac atcagatcca
     3121 ttaccactat ggccgctttc aggaatttca ccgtaaatca gaaaatactg ccatccatca
     3181 ttatttagaa gccttaaagg tcaaagacag atcacccctt cgcaccaaac tgacaagtgc
     3241 tctgaagaaa ttgtctacca agagactttg tcacaatgct ttagatgtgc agagtttaag
     3301 tgccctaggg tttgtttaca agctggaagg agaaaagagg caagctgctg agtactatga
     3361 gaaggcacaa aagatagatc cagaaaatgc agaattcctg actgctctct gtgagctccg
     3421 actttccatt tacccaactt tcttgtacaa agtggtgtga tctagaggat cccgggtggc
     3481 ttcgaattct cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     3541 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     3601 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     3661 agcagagatc cactttggcc gcggctcgag ggggttgggg ttgcgccttt tccaaggcag
     3721 ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc
     3781 cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc
     3841 tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt
     3901 gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc
     3961 agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag
     4021 cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg
     4081 gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc
     4141 tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca
     4201 gggggatcca ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     4261 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     4321 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     4381 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     4441 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     4501 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     4561 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     4621 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     4681 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     4741 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     4801 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
     4861 cgcacgaccc catgcatcgg tacctttaag accaatgact tacaaggcag ctgtagatct
     4921 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     4981 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     5041 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     5101 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5161 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     5221 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     5281 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5341 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     5401 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5461 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     5521 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     5581 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     5641 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     5701 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     5761 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5821 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5881 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5941 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6001 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6061 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6121 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6181 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     6241 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6301 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     6361 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     6421 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     6481 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     6541 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     6601 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6661 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     6721 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6781 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6841 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6901 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6961 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7021 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7081 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7141 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7201 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7261 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7321 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7381 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7441 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7501 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     7561 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7621 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7681 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7741 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7801 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7861 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7921 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7981 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8041 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8101 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     8161 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     8221 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     8281 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     8341 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     8401 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     8461 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     8521 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     8581 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     8641 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8701 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     8761 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8821 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     8881 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     8941 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     9001 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     9061 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     9121 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     9181 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     9241 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     9301 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     9361 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     9421 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     9481 agagaaaaaa gagcagtggg aat
//
