LOCUS       sequence-438797-        8508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for transient expression of pspCas9(BB)-2A-Puro nuclease and
            sgRNA targetting the C terminal of Zfp143 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8508)
  TITLE     mammalian expression plasmid for targetting of Zfp143 gene
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pX330-MM002-ZFP143-C-terminal-sgRNA
FEATURES             Location/Qualifiers
     source          1..8508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4019..4066
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4094..4111)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4100..4307
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4316..4456
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4316..4445
                     /label=AAV2 ITR
     rep_origin      4531..4986
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4618..4637)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4828..4849
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5003..5022)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5122..5144
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5182..5200)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5268..5372
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5373..5441,5442..6233)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5591..5610)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6404..6992
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6893..6912
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7054..7302
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7054..7074
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7225..7244
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7324..7399
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        7496..7781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          8061..8288
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      8300..8309
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8309..8374
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             8381..8401
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(8426..8508,1..4018)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(8426..8508,1..4018)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg
       61 agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag
      121 aagaagatac accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga
      181 gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga
      241 ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca
      301 cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc
      361 cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct
      421 gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt
      481 gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa
      541 ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct
      601 gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc tgggcctgac
      661 ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga
      721 cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct
      781 gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa
      841 caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca
      901 ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga
      961 gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg gagccagcca
     1021 ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact
     1081 gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag
     1141 catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt
     1201 ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc
     1261 ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag
     1321 cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg cttccgccca
     1381 gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc
     1441 caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca aagtgaaata
     1501 cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aggccatcgt
     1561 ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt
     1621 caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc
     1681 ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa
     1741 tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag
     1801 agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa
     1861 gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg
     1921 catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc
     1981 caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca
     2041 gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg
     2101 cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa
     2161 agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac
     2221 cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa
     2281 agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga
     2341 gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga
     2401 catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct ttctgaagga
     2461 cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca agagcgacaa
     2521 cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc tgctgaacgc
     2581 caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag gcggcctgag
     2641 cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa
     2701 gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct
     2761 gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga
     2821 tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct
     2881 gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt
     2941 gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat
     3001 cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga
     3061 gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac
     3121 cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat
     3181 gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc
     3241 tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa
     3301 gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt
     3361 ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat
     3421 ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga
     3481 agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg
     3541 ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc
     3601 ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc
     3661 cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat
     3721 catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa
     3781 agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat
     3841 catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac
     3901 caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca
     3961 ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg gaggcgacaa
     4021 aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagtaag aattcctaga
     4081 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     4141 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     4201 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     4261 gacagcaagg gggaggattg ggaagagaat agcaggcatg ctggggagcg gccgcaggaa
     4321 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     4381 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     4441 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4501 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     4561 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
     4621 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4681 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4741 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4801 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4861 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
     4921 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     4981 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     5041 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     5101 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     5161 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     5221 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5281 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5341 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5401 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5461 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5521 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5581 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5641 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5701 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5761 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5821 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5881 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5941 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6001 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6061 ctgataaatc tggagccggt gagcgtggaa gccgcggtat cattgcagca ctggggccag
     6121 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6181 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6241 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6301 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6361 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6421 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6481 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6541 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6601 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6661 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6721 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6781 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6841 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6901 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6961 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7021 tcctggcctt ttgctggcct tttgctcaca tgtgagggcc tatttcccat gattccttca
     7081 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac
     7141 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
     7201 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
     7261 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgagggttg gatgattaac
     7321 actgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
     7381 agtggcaccg agtcggtgct tttttgtttt agagctagaa atagcaagtt aaaataaggc
     7441 tagtccgttt ttagcgcgtg cgccaattct gcagacaaat ggctctagag gtacccgtta
     7501 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     7561 caatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     7621 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     7681 atgacggtaa atggcccgcc tggcattgtg cccagtacat gaccttatgg gactttccta
     7741 cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt
     7801 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
     7861 tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccgggggggg
     7921 gggggggggg gggggggggg ggsggggsgr ggsggrgrgg kgcggcggca gccaatcaga
     7981 gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa
     8041 agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg
     8101 ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg
     8161 ggcgggacgg cccttctcct ccgggctgta attagctgag caagaggtaa gggtttaagg
     8221 gatggttggt tggtggggta ttaatgttta attacctgga gcacctgcct gaaatcactt
     8281 tttttcaggt tggaccggtg ccaccatgga ctataaggac cacgacggag actacaagga
     8341 tcatgatatt gattacaaag acgatgacga taagatggcc ccaaagaaga agcggaaggt
     8401 cggtatccac ggagtcccag cagccgacaa gaagtacagc atcggcctgg acatcggcac
     8461 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccag
//
