LOCUS       sequence-438858-       14549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This is Caspase1 from mouse (NM_009807.2) with its CARD domain 
            replaced by the FKBP-V domain (dimerising domain)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Bonfim-Melo A, Noordstra I, Gupta S, Chan AH, Jones MJK, Schroder K,
            Yap AS
  TITLE     Rapid lamellipodial responses by neighbor cells drive epithelial 
            sealing in response to pyroptotic cell death.
  JOURNAL   Cell Rep. 2022 Feb 1;38(5):110316. doi: 
            10.1016/j.celrep.2022.110316.
  PUBMED    35108534
REFERENCE   2  (bases 1 to 14549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-02-1"; volume: "38(5):110316. doi"; pages: " 
            10.1016/j.celrep.2022.110316"
COMMENT     Sequence Label: pTripz Casp1-IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..14549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2998..3007
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3025..3345
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4279..4305
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(4317..4891,4892..4894,4895..4903)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             4910..5620
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5055..5073)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5323..5342
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     ncRNA           5642..5735
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           5761..5873
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(5968..5986)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        6000..6510
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6429..6447
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    complement(6523..6556)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             6563..7270
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(7271..7304)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    7327..7899
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             7902..8504
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8401..8421
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8520..9108
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8573..8593)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8991..9002)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9315..9548
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9649..9666)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9655..9879
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9925..10353
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10012..10031)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10222..10243
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10362..10382)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10367..10696
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10547..10682
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10609..10628
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    11904..12025
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12074..12090)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12074..12090)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12087..12109)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12098..12114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12122..12128,12129..12146,12147..12152))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12167..12188
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12305..12322)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12476..13064)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12556..12575)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13235..14026,14027..14095))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13858..13877
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14096..14200)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    14248..14382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggtgcc
     3001 accatgggta ccatggcatc tagaggagtg caggtggaaa ccatctcccc aggagacggg
     3061 cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact acaccgggat gcttgaagat
     3121 ggaaagaaag tcgattcctc ccgggacaga aacaagccct ttaagtttat gctaggcaag
     3181 caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc
     3241 aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg catcatccca
     3301 ccacatgcca ctctcgtctt cgatgtggag cttctaaaac tggaaactag tgctccatca
     3361 gctgaaacat ttgttgctac agaagattct aaaggaggac atccttcatc ctcagaaaca
     3421 aaggaagaac agaacaaaga agatggcaca tttccaggac tgactgggac cctcaagttt
     3481 tgccctttag aaaaagccca gaagttatgg aaagaaaatc cttcagagat ttatccaata
     3541 atgaatacaa ccactcgtac acgtcttgcc ctcattatct gcaacacaga gtttcaacat
     3601 ctttctccga gggttggagc tcaagttgac ctcagagaaa tgaagttgct gctggaggat
     3661 ctggggtata ccgtgaaagt gaaagaaaat ctcacagctc tggagatggt gaaagaggtg
     3721 aaagaatttg ctgcctgccc agagcacaag acttctgaca gtactttcct tgtattcatg
     3781 tctcatggta tccaggaggg aatatgtggg accacatact ctaatgaagt ttcagatatt
     3841 ttaaaggttg acacaatctt tcagatgatg aacactttga agtgcccaag cttgaaagac
     3901 aagcccaagg tgatcattat tcaggcatgc cgtggagaga aacaaggagt ggtgttgtta
     3961 aaagattcag taagagactc tgaagaggat ttcttaacgg atgcaatttt tgaagatgat
     4021 ggcattaaga aggcccatat agagaaagat tttattgctt tctgctcttc aacaccagat
     4081 aatgtgtctt ggagacatcc tgtcaggggc tcacttttca ttgagtcact catcaaacac
     4141 atgaaagaat atgcctggtc ttgtgacttg gaggacattt tcagaaaggt tcgattttca
     4201 tttgaacaac cagaatttag gctacagatg cccactgctg atagggtgac cctgacaaaa
     4261 cgtttctacc tcttcccgta tccttatgac gtgcctgact atgcctgagc ggccgccccc
     4321 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     4381 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     4441 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     4501 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     4561 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     4621 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     4681 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     4741 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtacaca
     4801 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     4861 gtggttttcc tttgaaaaac acgatgataa tatggccaca accgaattca tggtgagcaa
     4921 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     4981 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     5041 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     5101 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     5161 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     5221 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     5281 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     5341 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     5401 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     5461 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     5521 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     5581 ccgccactcc accggcggca tggacgagct gtacaagtga gtttaaacat cgatagtttg
     5641 tttgaatgag gcttcagtac tttacagaat cgttgcctgc acatcttgga aacacttgct
     5701 gggattactt cttcaggtta acccaacaga aggctcgagg taaccggatc ctgatcagaa
     5761 ttcaaggggc tactttagga gcaattatct tgtttactaa aactgaatac cttgctatct
     5821 ctttgataca tttttacaaa gctgaattaa aatggtataa attaaatcac ttttttcaat
     5881 tggaagacta atgcggccgg ccattactcc gtctcgtgtc ttgttgcata tgtctgctgg
     5941 tttgtttgat gttgtttgcg ggcgggccct atagtgagtc gtattaccta ggacgcgtgg
     6001 gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct
     6061 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc
     6121 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct
     6181 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg
     6241 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg
     6301 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc
     6361 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg
     6421 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc
     6481 gttgaccgaa tcaccgacct ctctccccag gctagcggat ccataacttc gtatagtata
     6541 cattatacga agttatgcca ccatgtctag gctggacaag agcaaagtca taaacggagc
     6601 tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa
     6661 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga
     6721 tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga
     6781 gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca
     6841 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct
     6901 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc
     6961 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt
     7021 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc
     7081 aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat
     7141 catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga
     7201 cgattttgac ttagacatgc tcccagccga tgcccttgac gattttgacc ttgacatgct
     7261 ccccgggtaa ataacttcgt atagtataca ttatacgaag ttatggatcc gcggccgcaa
     7321 attccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     7381 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     7441 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     7501 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     7561 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     7621 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     7681 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     7741 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     7801 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     7861 ccacggggac gtggttttcc tttgaaaaac acgataatac catggccacc gagtacaagc
     7921 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg
     7981 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg
     8041 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     8101 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     8161 cggtgttcgc cgagatcggc tcgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     8221 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     8281 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     8341 ccggagtgga ggcggccgag cgcgctgggg tgcccgcctt cctggagacc tccgcgcccc
     8401 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     8461 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagttcgc gtctggaaca
     8521 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     8581 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     8641 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     8701 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     8761 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     8821 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     8881 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     8941 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     9001 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     9061 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt
     9121 ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     9181 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     9241 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     9301 agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     9361 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     9421 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     9481 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     9541 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     9601 aatatcagag agtgagaggc cttgacattg tttaaacccg ctgatcagcc tcgactgtgc
     9661 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     9721 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     9781 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     9841 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     9901 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     9961 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
    10021 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
    10081 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
    10141 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
    10201 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
    10261 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
    10321 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
    10381 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    10441 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    10501 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
    10561 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    10621 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    10681 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
    10741 cgtgatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
    10801 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
    10861 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
    10921 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
    10981 cattggggaa ttaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
    11041 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
    11101 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
    11161 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
    11221 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
    11281 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
    11341 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
    11401 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
    11461 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
    11521 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
    11581 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
    11641 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
    11701 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
    11761 gggcaaagga atagcacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
    11821 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
    11881 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
    11941 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    12001 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
    12061 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    12121 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    12181 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    12241 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    12301 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    12361 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    12421 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    12481 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    12541 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    12601 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    12661 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    12721 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    12781 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    12841 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    12901 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    12961 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    13021 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    13081 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    13141 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    13201 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    13261 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    13321 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    13381 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    13441 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    13501 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    13561 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    13621 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    13681 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    13741 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    13801 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    13861 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    13921 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    13981 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    14041 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    14101 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    14161 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    14221 cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa
    14281 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    14341 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
    14401 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
    14461 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
    14521 tgttaaatat gtactacaaa cttagtagt
//
