LOCUS       sequence-438881-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses autophagy flux LC3B fluorescence reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Ortiz ANR, Zhang L, Ash PEA, Basu A, Puri S, van der Spek SJF, Wang 
            Z, Dorrian L, Emili A, Wolozin B
  TITLE     Proximity labeling reveals dynamic changes in the SQSTM1 protein 
            network.
  JOURNAL   bioRxiv [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324.
  PUBMED    38168279
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324."
COMMENT     Sequence Label: pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(76..95)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     423..444
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             514..888
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     primer_bind     complement(968..988)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
       61 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      121 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      181 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
      241 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      301 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      361 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
      421 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      481 tacaagtccg gactcagatc cggtaccgaa ttcatgccgt cggagaagac cttcaagcag
      541 cgccgcacct tcgaacaaag agtagaagat gtccgactta ttcgagagca gcatccaacc
      601 aaaatcccgg tgataataga acgatacaag ggtgagaagc agcttcctgt tctggataaa
      661 acaaagttcc ttgtacctga ccatgtcaac atgagtgagc tcatcaagat aattagaagg
      721 cgcttacagc tcaatgctaa tcaggccttc ttcctgttgg tgaacggaca cagcatggtc
      781 agcgtctcca caccaatctc agaggtgtat gagagtgaga aagatgaaga tggattcctg
      841 tacatggtct atgcctccca ggagacgttc gggatgaaat tgtcagtgta actcgagcac
      901 cagaattcgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
      961 ttaactatgt tgctcctttt acgctatg
//
