LOCUS       sequence-438883-        1303 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-tagged TurboID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Ortiz ANR, Zhang L, Ash PEA, Basu A, Puri S, van der Spek SJF, Wang 
            Z, Dorrian L, Emili A, Wolozin B
  TITLE     Proximity labeling reveals dynamic changes in the SQSTM1 protein 
            network.
  JOURNAL   bioRxiv [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324.
  PUBMED    38168279
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324."
COMMENT     Sequence Label: pHR-SFFV-FLAG-TurboID
FEATURES             Location/Qualifiers
     source          1..1303
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             72..95
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             96..1052
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    1053..1118
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 actgagtcgc ccggggggga tctggagctc tcgagaattc tcacgcgtgc tagctaccgg
       61 tagccaccat ggactacaag gatgacgacg ataagaaaga caatactgtg cctctgaagc
      121 tgatcgctct cctggctaat ggcgagttcc atagtggcga acagctggga gaaaccctgg
      181 gcatgtccag ggccgctatc aacaagcaca ttcagactct gcgcgactgg ggcgtggacg
      241 tgttcaccgt gcccggaaag ggctactctc tgcccgagcc tatcccgctg ctgaacgcta
      301 aacagattct gggacagctg gacggcggga gcgtggcagt cctgcctgtg gtcgactcca
      361 ccaatcagta cctgctggat cgaatcggcg agctgaagag tggggatgct tgcattgcag
      421 aatatcagca ggcagggaga ggaagcagag ggaggaaatg gttctctcct tttggagcta
      481 acctgtacct gagtatgttt tggcgcctga agcggggacc agcagcaatc ggcctgggcc
      541 cggtcatcgg aattgtcatg gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag
      601 tcaaatggcc caatgacctg tatctgcagg atagaaagct ggcaggcatc ctggtggagc
      661 tggccggaat aacaggcgat gctgcacaga tcgtcattgg cgccgggatt aacgtggcta
      721 tgaggcgcgt ggaggaaagc gtggtcaatc agggctggat cacactgcag gaagcaggga
      781 ttaacctgga caggaatact ctggccgcta cgctgatccg agagctgcgg gcagccctgg
      841 aactgttcga gcaggaaggc ctggctccat atctgccacg gtgggagaag ctggataact
      901 tcatcaatag acccgtgaag ctgatcattg gggacaaaga gattttcggg attagccggg
      961 ggattgataa acagggagcc ctgctgctgg aacaggacgg agttatcaaa ccctggatgg
     1021 gcggagaaat cagtctgcgg tctgccgaaa agtccggact cagatctcga gctcaagctt
     1081 cgaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga taactgatta
     1141 agatccttga cttgcggccg caactcccac ctgcaacatg cgtgactgac tgaggccgcg
     1201 actctagagt cgacctgcag gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc
     1261 tggattacaa aatttgtgaa agattgactg gtattcttaa cta
//
