LOCUS       sequence-438902-        2626 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG-TurboID-mutLIR-SQSTM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ortiz ANR, Zhang L, Ash PEA, Basu A, Puri S, van der Spek SJF, Wang 
            Z, Dorrian L, Emili A, Wolozin B
  TITLE     Proximity labeling reveals dynamic changes in the SQSTM1 protein 
            network.
  JOURNAL   bioRxiv [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324.
  PUBMED    38168279
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324."
COMMENT     Sequence Label: pHR-SFFV-FLAG-TurboID-mutLIR-SQSTM1
FEATURES             Location/Qualifiers
     source          1..2626
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      66..75
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             75..98
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             99..1055
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     primer_bind     complement(2606..2626)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 cctccgacag actgagtcgc ccggggggga tctggagctc tcgagaattc tcacgcgtac
       61 cggtagccac catggactac aaggatgacg acgataagaa agacaatact gtgcctctga
      121 agctgatcgc tctcctggct aatggcgagt tccatagtgg cgaacagctg ggagaaaccc
      181 tgggcatgtc cagggccgct atcaacaagc acattcagac tctgcgcgac tggggcgtgg
      241 acgtgttcac cgtgcccgga aagggctact ctctgcccga gcctatcccg ctgctgaacg
      301 ctaaacagat tctgggacag ctggacggcg ggagcgtggc agtcctgcct gtggtcgact
      361 ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa gagtggggat gcttgcattg
      421 cagaatatca gcaggcaggg agaggaagca gagggaggaa atggttctct ccttttggag
      481 ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg accagcagca atcggcctgg
      541 gcccggtcat cggaattgtc atggcagaag cgctgcgaaa gctgggagca gacaaggtgc
      601 gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa gctggcaggc atcctggtgg
      661 agctggccgg aataacaggc gatgctgcac agatcgtcat tggcgccggg attaacgtgg
      721 ctatgaggcg cgtggaggaa agcgtggtca atcagggctg gatcacactg caggaagcag
      781 ggattaacct ggacaggaat actctggccg ctacgctgat ccgagagctg cgggcagccc
      841 tggaactgtt cgagcaggaa ggcctggctc catatctgcc acggtgggag aagctggata
      901 acttcatcaa tagacccgtg aagctgatca ttggggacaa agagattttc gggattagcc
      961 gggggattga taaacaggga gccctgctgc tggaacagga cggagttatc aaaccctgga
     1021 tgggcggaga aatcagtctg cggtctgccg aaaagtccgg actcagatct ggtggcagtg
     1081 gatctcgagt catggcgtcg ctcaccgtga aggcctacct tctgggcaag gaggacgcgg
     1141 cgcgcgagat tcgccgcttc agcttctgct gcagccccga gcctgaggcg gaagccgagg
     1201 ctgcggcggg tccgggaccc tgcgagcggc tgctgagccg ggtggccgcc ctgttccccg
     1261 cgctgcggcc tggcggcttc caggcgcact accgcgatga ggacggggac ttggttgcct
     1321 tttccagtga cgaggaattg acaatggcca tgtcctacgt gaaggatgac atcttccgaa
     1381 tctacattaa agagaaaaaa gagtgccggc gggaccaccg cccaccgtgt gctcaggagg
     1441 cgccccgcaa catggtgcac cccaatgtga tctgcgatgg ctgcaatggg cctgtggtag
     1501 gaacccgcta caagtgcagc gtctgcccag actacgactt gtgtagcgtc tgcgagggaa
     1561 agggcttgca ccgggggcac accaagctcg cattccccag ccccttcggg cacctgtctg
     1621 agggcttctc gcacagccgc tggctccgga aggtgaaaca cggacacttc gggtggccag
     1681 gatgggaaat gggtccacca ggaaactgga gcccacgtcc tcctcgtgca ggggaggccc
     1741 gccctggccc cacggcagaa tcagcttctg gtccatcgga ggatccgagt gtgaatttcc
     1801 tgaagaacgt tggggagagt gtggcagctg cccttagccc tctgggcatt gaagttgata
     1861 tcgatgtgga gcacggaggg aaaagaagcc gcctgacccc cgtctctcca gagagttcca
     1921 gcacagagga gaagagcagc tcacagccaa gcagctgctg ctctgatccc agcaagccgg
     1981 gtgggaatgt tgagggcgcc acgcagtctc tggcggagca gatgagaaag atcgccttgg
     2041 agtccgaggg gcgccctgag gaacagatgg agtcggataa ctgttcagga ggagccgctg
     2101 cagctaccca tctgtcttca aaagaagtgg acccgtctac aggtgaactc cagtccctac
     2161 agatgccaga atccgaaggg ccaagctctc tggacccctc ccaggaggga cccacagggc
     2221 tgaaggaagc tgccttgtac ccacatctcc cgccagaggc tgacccgcgg ctgattgagt
     2281 ccctctccca gatgctgtcc atgggcttct ctgatgaagg cggctggctc accaggctcc
     2341 tgcagaccaa gaactatgac atcggagcgg ctctggacac catccagtat tcaaagcatc
     2401 ccccgccgtt gtaaggatcc accggatcta gataactgat taagatcctt gacttgcggc
     2461 cgcaactccc acctgcaaca tgcgtgactg actgaggccg cgactctaga gtcgacctgc
     2521 aggcatgcaa gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     2581 aaagattgac tggtattctt aactatgttg ctccttttac gctatg
//
