LOCUS       sequence-438905-        2601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG-TurboID-mutKIR-SQSTM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ortiz ANR, Zhang L, Ash PEA, Basu A, Puri S, van der Spek SJF, Wang 
            Z, Dorrian L, Emili A, Wolozin B
  TITLE     Proximity labeling reveals dynamic changes in the SQSTM1 protein 
            network.
  JOURNAL   bioRxiv [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324.
  PUBMED    38168279
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Jun 27:2023.12.12.571324. doi: 
            10.1101/2023.12.12.571324."
COMMENT     Sequence Label: pHR-SFFV-FLAG-TurboID-mutKIR-SQSTM1
FEATURES             Location/Qualifiers
     source          1..2601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             50..73
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             74..1030
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     primer_bind     complement(2581..2601)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ggggatctgg agctctcgag aattctcacg cgtaccggta gccaccatgg actacaagga
       61 tgacgacgat aagaaagaca atactgtgcc tctgaagctg atcgctctcc tggctaatgg
      121 cgagttccat agtggcgaac agctgggaga aaccctgggc atgtccaggg ccgctatcaa
      181 caagcacatt cagactctgc gcgactgggg cgtggacgtg ttcaccgtgc ccggaaaggg
      241 ctactctctg cccgagccta tcccgctgct gaacgctaaa cagattctgg gacagctgga
      301 cggcgggagc gtggcagtcc tgcctgtggt cgactccacc aatcagtacc tgctggatcg
      361 aatcggcgag ctgaagagtg gggatgcttg cattgcagaa tatcagcagg cagggagagg
      421 aagcagaggg aggaaatggt tctctccttt tggagctaac ctgtacctga gtatgttttg
      481 gcgcctgaag cggggaccag cagcaatcgg cctgggcccg gtcatcggaa ttgtcatggc
      541 agaagcgctg cgaaagctgg gagcagacaa ggtgcgagtc aaatggccca atgacctgta
      601 tctgcaggat agaaagctgg caggcatcct ggtggagctg gccggaataa caggcgatgc
      661 tgcacagatc gtcattggcg ccgggattaa cgtggctatg aggcgcgtgg aggaaagcgt
      721 ggtcaatcag ggctggatca cactgcagga agcagggatt aacctggaca ggaatactct
      781 ggccgctacg ctgatccgag agctgcgggc agccctggaa ctgttcgagc aggaaggcct
      841 ggctccatat ctgccacggt gggagaagct ggataacttc atcaatagac ccgtgaagct
      901 gatcattggg gacaaagaga ttttcgggat tagccggggg attgataaac agggagccct
      961 gctgctggaa caggacggag ttatcaaacc ctggatgggc ggagaaatca gtctgcggtc
     1021 tgccgaaaag tccggactca gatctggtgg cagtggatct cgagtcatgg cgtcgctcac
     1081 cgtgaaggcc taccttctgg gcaaggagga cgcggcgcgc gagattcgcc gcttcagctt
     1141 ctgctgcagc cccgagcctg aggcggaagc cgaggctgcg gcgggtccgg gaccctgcga
     1201 gcggctgctg agccgggtgg ccgccctgtt ccccgcgctg cggcctggcg gcttccaggc
     1261 gcactaccgc gatgaggacg gggacttggt tgccttttcc agtgacgagg aattgacaat
     1321 ggccatgtcc tacgtgaagg atgacatctt ccgaatctac attaaagaga aaaaagagtg
     1381 ccggcgggac caccgcccac cgtgtgctca ggaggcgccc cgcaacatgg tgcaccccaa
     1441 tgtgatctgc gatggctgca atgggcctgt ggtaggaacc cgctacaagt gcagcgtctg
     1501 cccagactac gacttgtgta gcgtctgcga gggaaagggc ttgcaccggg ggcacaccaa
     1561 gctcgcattc cccagcccct tcgggcacct gtctgagggc ttctcgcaca gccgctggct
     1621 ccggaaggtg aaacacggac acttcgggtg gccaggatgg gaaatgggtc caccaggaaa
     1681 ctggagccca cgtcctcctc gtgcagggga ggcccgccct ggccccacgg cagaatcagc
     1741 ttctggtcca tcggaggatc cgagtgtgaa tttcctgaag aacgttgggg agagtgtggc
     1801 agctgccctt agccctctgg gcattgaagt tgatatcgat gtggagcacg gagggaaaag
     1861 aagccgcctg acccccgtct ctccagagag ttccagcaca gaggagaaga gcagctcaca
     1921 gccaagcagc tgctgctctg atcccagcaa gccgggtggg aatgttgagg gcgccacgca
     1981 gtctctggcg gagcagatga gaaagatcgc cttggagtcc gaggggcgcc ctgaggaaca
     2041 gatggagtcg gataactgtt caggaggaga tgatgactgg acccatctgt cttcaaaaga
     2101 agtggccccg tctgctgctg cgctccagtc cctacagatg ccagaatccg aagggccaag
     2161 ctctctggac ccctcccagg agggacccac agggctgaag gaagctgcct tgtacccaca
     2221 tctcccgcca gaggctgacc cgcggctgat tgagtccctc tcccagatgc tgtccatggg
     2281 cttctctgat gaaggcggct ggctcaccag gctcctgcag accaagaact atgacatcgg
     2341 agcggctctg gacaccatcc agtattcaaa gcatcccccg ccgttgtaag gatccaccgg
     2401 atctagataa ctgattaaga tccttgactt gcggccgcaa ctcccacctg caacatgcgt
     2461 gactgactga ggccgcgact ctagagtcga cctgcaggca tgcaagcttg atatcaagct
     2521 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2581 tgttgctcct tttacgctat g
//
