LOCUS       sequence-438921-        4458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC5A6_STOP
FEATURES             Location/Qualifiers
     source          1..4458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..85
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(244..271)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(363..449)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     498..520
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     512..529
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     513..529
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    545..644
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2559..2658)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2675..2694)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2676..2694)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2699..2715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2699..2715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2817..2836)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2828..3637
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2895..2914)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3784..4372
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4273..4292
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
       61 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
      121 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
      181 gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac
      241 gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc
      301 gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc
      361 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
      421 tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac
      481 gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg
      541 gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac acattgatga
      601 gcaatgcttt tttataatgc caactttgta caaaaaagca ggctccacca tgtccgtggg
      661 cgtgtccaca tctgccccac tgtcccctac ctctggcaca agcgtgggca tgtccacctt
      721 ttctatcatg gactacgtgg tgttcgtgct gctgctggtg ctgtctctgg caatcggact
      781 gtatcacgca tgcaggggat ggggccggca caccgtggga gagctgctga tggccgatcg
      841 gaagatgggc tgtctgcctg tggccctgtc tctgctggcc acattccaga gcgccgtggc
      901 aatcctggga gtgccatccg agatctacag attcggcacc cagtattggt ttctgggctg
      961 ctgttacttc ctgggcctgc tgatccctgc ccacatcttc atcccagtgt tttataggct
     1021 gcacctgaca agcgcctacg agtatctgga gctgcggttc aacaagaccg tgagggtgtg
     1081 cggcaccgtg acattcatct ttcagatggt catctacatg ggagtggtgc tgtatgcacc
     1141 atccctggcc ctgaatgcag tgaccggctt cgacctgtgg ctgtctgtgc tggccctggg
     1201 catcgtgtgc accgtgtaca cagccctggg aggcctgaag gccgtgatct ggacagacgt
     1261 gttccagacc ctggtcatgt tcctgggcca gctggccgtg atcatcgtgg gctccgccaa
     1321 agtgggagga ctgggacgcg tgtgggcagt ggccagccag cacggccgga tctccggctt
     1381 tgagctggac cctgatccat tcgtgcgcca caccttttgg acactggcct tcggcggcgt
     1441 gtttatgatg ctgagcctgt acggcgtgaa ccaggcacag gtgcagcggt acctgagctc
     1501 caggaccgag aaggcagccg tgctgtcttg ctacgccgtg ttcccctttc agcaggtgag
     1561 cctgtgcgtg ggctgtctga tcggcctggt catgttcgcc tactatcagg agtaccctat
     1621 gtccatccag caggcacagg cagcaccaga ccagttcgtg ctgtattttg tgatggatct
     1681 gctgaaggga ctgccaggac tgccaggact gttcatcgca tgcctgttta gcggctccct
     1741 gtctaccatc tctagcgcct ttaacagcct ggccaccgtg acaatggagg acctgatccg
     1801 gccttggttc ccagagtttt ccgaggcaag ggcaatcatg ctgtctaggg gactggcctt
     1861 cggctacgga ctgctgtgcc tgggaatggc ctatatctcc tctcagatgg gccctgtgct
     1921 gcaggccgcc atcagcatct ttggaatggt gggaggacca ctgctgggac tgttctgcct
     1981 gggcatgttc tttccttgtg ccaatccacc tggagcagtg gtgggactgc tggcaggcct
     2041 ggtcatggcc ttttggatcg gcatcggcag catcgtgaca tccatgggca gctccatgcc
     2101 acccagccca tccaacggct ctagcttcag cctgcccacc aatctgacag tggccaccgt
     2161 gaccacactg atgcccctga ccacattttc caagcctacc ggcctgcaga gattctactc
     2221 tctgagctac ctgtggtatt ctgcccacaa cagcaccaca gtgatcgtgg tgggcctgat
     2281 cgtgagcctg ctgacaggcc ggatgagagg caggtccctg aatccagcca ccatctaccc
     2341 cgtgctgcct aagctgctgt ccctgctgcc cctgtcttgc cagaagagac tgcactgtag
     2401 gtcttatggc caggaccacc tggatacagg cctgttccca gagaagccca gaaatggcgt
     2461 gctgggcgac agcagggata aggaggccat ggccctggat ggcaccgcct atcagggctc
     2521 ctctagcaca tgtatcctgc aggagaccag cctgtaagac ccagctttct tgtacaaagt
     2581 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2641 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     2701 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     2761 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     2821 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
     2881 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     2941 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     3001 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     3061 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     3121 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     3181 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     3241 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     3301 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     3361 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     3421 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     3481 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     3541 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     3601 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     3661 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     3721 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3781 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3841 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3901 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3961 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4021 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4081 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4141 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4201 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4261 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4321 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4381 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4441 tgcgttatcc cctgattc
//
