LOCUS       sequence-43893-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens 
            L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW
  TITLE     A mammalian mediator subunit that shares properties with 
            Saccharomyces cerevisiae mediator subunit Cse2.
  JOURNAL   J Biol Chem. 2004 Feb 13. 279(7):5846-51.
  PUBMED    14638676
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2004 Feb 13. 279(7):5846-51."
COMMENT     Sequence Label: GST-MED9
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..49
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             59..76
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             92..136
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             167..181
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             641..664
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(727..745)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      741..788
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctcaatcgga tggttcacta gtggttctgg tcatcaccat caccatcact ccgcgggtct
       61 ggtgccacgc ggtagtactg caattggtat gaaagaaacc gctgctgcta aattcgaacg
      121 ccagcacatg gacagcccag atctgggtac cggtggtggc tccggtgatg acgacgacaa
      181 gagtcccatg ggatatcggg gatccatggc ttcctctggg gtggcgggtg ggagacaggc
      241 cgaagatacg ctacagccac cgcccgagct gctgccggag tccaagccgc caccaccgcc
      301 tcagcctctg ccggtcgctg cgctcccgcc gccggcggct ccgcggcctc agtcccccgc
      361 tggcgcgaag gaggagaatt actccttttt gccgttggtt cacaatgtca tcaaatgcat
      421 ggacaaggac agcccggatc tccaccagga cctgaacgcc ctcaaaacca agttccagga
      481 gctgcggaag ctcatcggca ccatgcccgg catccacgtg agccccgagc agcagcagca
      541 gcagctccac agcctccgag agcaagtgag gaccaagaac gagctgctgc agaagtacaa
      601 gagcctctgc atgtttgaga tccccaagga ctagctcgag caccaccacc accaccacca
      661 ccactaattg attaatacct aggctgctaa acaaagcccg aaaggaagct gagttggctg
      721 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
      781 gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg
      841 cgcattaaac gcggcgggtg tggtggttac
//
