LOCUS       sequence-438935-       11138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To visualize free autophagosomes (GFP and mCherry fluorescence) and 
            autophagosomes that have fused with the lysosome (autolyosomes; 
            mCherry fluorescence only, due to acid sensitivity of GFP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11138)
  AUTHORS   Leeman DS, Hebestreit K, Ruetz T, Webb AE, McKay A, Pollina EA, 
            Dulken BW, Zhao X, Yeo RW, Ho TT, Mahmoudi S, Devarajan K, Passegue 
            E, Rando TA, Frydman J, Brunet A
  TITLE     Lysosome activation clears aggregates and enhances quiescent neural 
            stem cell activation during aging.
  JOURNAL   Science. 2018 Mar 16;359(6381):1277-1283. doi: 
            10.1126/science.aag3048. Epub 2018 Mar 15.
  PUBMED    29590078
REFERENCE   2  (bases 1 to 11138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aag3048"; journalName: "Science"; date: 
            "2018-03-16- 16"; volume: "359"; issue: "6381"; pages: "1277-1283"
COMMENT     Sequence Label: FUW mCherry-GFP-LC3
FEATURES             Location/Qualifiers
     source          1..11138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     89..109
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             152..332
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    379..504
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    997..1230
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1415..1459
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1757..1874
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1939..3150
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2987..3006
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3189..3198
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3195..3902
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3340..3358)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3608..3627
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    3930..3954
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3963..3972
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3969..3971,3972..3974,3975..4685)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3969..3971,3972..3974,3975..4685)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4014..4035)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4275..4294)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4622..4643
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4713..5087
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     misc_feature    5147..5735
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5200..5220)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5618..5629)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5738..5754)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5739..5755)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6260..6440
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6466..6483)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6472..6696
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6742..7170
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6829..6848)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7039..7060
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7179..7199)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7184..7513
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7364..7499
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7426..7445
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7561..7608
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7627..8001
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8131..8252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8168..8187)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8222..8241
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8301..8317)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8301..8317)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8314..8336)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8325..8341
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8349..8355,8356..8373,8374..8379))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8394..8415
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8532..8549)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8703..9291)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8783..8802)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9462..10253,10254..10322))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10085..10104
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10323..10427)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10502..10521)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10693..11072
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(11074..11138,1..134)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
       61 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      121 gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc
      181 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
      241 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
      301 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
      361 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
      421 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
      481 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
      541 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
      601 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
      661 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
      721 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
      781 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
      841 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
      901 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
      961 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     1021 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     1081 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     1141 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     1201 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     1261 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     1321 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     1381 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     1441 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     1501 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     1561 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     1621 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     1681 cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg
     1741 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     1801 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     1861 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaacc
     1921 cgtgtcggct ccagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc
     1981 tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg
     2041 cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc
     2101 agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag
     2161 agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt
     2221 ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt
     2281 cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga
     2341 gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga
     2401 agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac
     2461 tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc
     2521 ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac
     2581 ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg
     2641 gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc
     2701 tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc
     2761 gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc
     2821 cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta
     2881 gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg
     2941 cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt
     3001 tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa
     3061 atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa
     3121 ttctggccgt ttttggcttt tttgttagac gaagcttggg ctgcaggtcg actctagagg
     3181 atccggtcgc caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     3241 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     3301 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     3361 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     3421 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     3481 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     3541 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     3601 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     3661 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     3721 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     3781 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     3841 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     3901 agtccggact cagatccgct ggcccaggaa caagtttgta caaaaaagca ggctttccgg
     3961 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     4021 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     4081 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     4141 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     4201 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     4261 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     4321 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     4381 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     4441 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     4501 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     4561 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     4621 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     4681 acaagtccgg actcagatcc ggtaccgaat tcatgccgtc ggagaagacc ttcaagcagc
     4741 gccgcacctt cgaacaaaga gtagaagatg tccgacttat tcgagagcag catccaacca
     4801 aaatcccggt gataatagaa cgatacaagg gtgagaagca gcttcctgtt ctggataaaa
     4861 caaagttcct tgtacctgac catgtcaaca tgagtgagct catcaagata attagaaggc
     4921 gcttacagct caatgctaat caggccttct tcctgttggt gaacggacac agcatggtca
     4981 gcgtctccac accaatctca gaggtgtatg agagtgagaa agatgaagat ggattcctgt
     5041 acatggtcta tgcctcccag gagacgttcg ggatgaaatt gtcagtgtaa gtcgagcatg
     5101 catctagacc cagcggcgcg ccgaattcga tatcaagctt atcgataatc aacctctgga
     5161 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5221 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5281 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5341 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5401 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5461 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5521 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     5581 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     5641 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     5701 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct
     5761 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     5821 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     5881 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     5941 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     6001 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
     6061 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
     6121 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     6181 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
     6241 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     6301 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     6361 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     6421 agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     6481 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     6541 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     6601 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     6661 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     6721 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6781 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6841 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6901 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6961 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     7021 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     7081 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7141 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     7201 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7261 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7321 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     7381 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7441 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7501 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     7561 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     7621 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
     7681 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
     7741 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     7801 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
     7861 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
     7921 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     7981 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
     8041 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8101 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     8161 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8221 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     8281 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8341 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8401 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8461 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8521 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8581 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     8641 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8701 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8761 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8821 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8881 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8941 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9001 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9061 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9121 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     9181 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9241 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9301 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9361 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9421 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9481 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9541 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9601 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9661 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9721 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9781 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9841 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9901 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9961 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10021 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    10081 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10141 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10201 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10261 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10321 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10381 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10441 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
    10501 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
    10561 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
    10621 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
    10681 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
    10741 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
    10801 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
    10861 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
    10921 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
    10981 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
    11041 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
    11101 ggcgtggata gcggtttgac tcacggggat ttccaagt
//
