LOCUS       sequence-438936-        8648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle vector for baculovirus production, using FlashBac bacmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Wang J, Choi JM, Holehouse AS, Lee HO, Zhang X, Jahnel M, Maharana 
            S, Lemaitre R, Pozniakovsky A, Drechsel D, Poser I, Pappu RV, 
            Alberti S, Hyman AA
  TITLE     A Molecular Grammar Governing the Driving Forces for Phase 
            Separation of Prion-like RNA Binding Proteins.
  JOURNAL   Cell. 2018 Jul 26;174(3):688-699.e16. doi: 
            10.1016/j.cell.2018.06.006. Epub 2018 Jun 28.
  PUBMED    29961577
REFERENCE   2  (bases 1 to 8648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.06.006"; journalName: "Cell"; date: 
            "2018-07-26- 26"; volume: "174"; issue: "3"; pages: "688-699.e16"
COMMENT     Sequence Label: 
            TH1258-013_Jie_TAF15_pOCC119_pOEM1-N-HIS6-MBP-NotI-AscI-C-TEV-mGFP
FEATURES             Location/Qualifiers
     source          1..8648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        79..170
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     95..112
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     regulatory      212..221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             230..247
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             260..1360
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1334..1357
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1415..1438
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3242..3262
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(3263..3265,3266..3268,3269..3982)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3308..3329)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3569..3588)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3916..3937
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4115..4249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4152..4171)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4206..4225
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     3'UTR           4322..4694
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     primer_bind     complement(5046..5063)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5156..5744)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5236..5255)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5915..6706,6707..6775))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6538..6557
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6776..6880)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7181..7203
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7195..7212
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7196..7212
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7223..7242
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7223..7241
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7852..7976
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(8014..8619)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 agctgccacc attttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat
       61 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
      121 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
      181 attcataccg tcccaccatc gggcgcggat cgccaccatg ggcagcagcc atcaccatca
      241 ccatcacagc agcggcagaa tgaaaatcga agaaggtaaa ctggtaatct ggattaacgg
      301 cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat
      361 taaagtcacc gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac
      421 tggcgatggc cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc
      481 tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac
      541 ctgggatgcc gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt
      601 atcgctgatt tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc
      661 ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga
      721 accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa
      781 cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac
      841 cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc
      901 agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc
      961 caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca
     1021 accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa
     1081 agagctggca aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt
     1141 taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggtgaa
     1201 agatccgcgt attgccgcca ctatggaaaa cgcccagaaa ggtgaaatca tgccgaacat
     1261 cccgcagatg tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg
     1321 tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact aattcgagct cgaacaacaa
     1381 caacaataac aataacaaca acagcagcgg cagactggaa gttctgttcc aggggccggc
     1441 ggccgcaatg agcgatagtg gaagttacgg acagtcggga ggcgagcagc aaagctacag
     1501 cacctacgga aaccctggaa gtcaaggata cggacaggct tcccagagct actctggcta
     1561 cggacagacc actgactcca gctacggaca gaactactct ggttactctt catacggcca
     1621 gtcatactcc cagagctacg gtggctacga gaaccagaag cagtccagct actcacagca
     1681 gccatacaac aaccagggcc agcagcagaa catggaatct tcaggttctc agggaggtcg
     1741 cgccccttca tacgaccagc ccgactacgg tcagcaggac tcctacgacc agcagagcgg
     1801 ttacgaccag caccagggct cttacgacga gcagtcaaac tacgaccagc agcacgactc
     1861 ttactcacag aaccagcagt cttaccactc acagcgcgag aactactctc accacactca
     1921 agacgacagg agggacgtga gcaggtacgg tgaagacaac aggggttacg gcggaagcca
     1981 gggtggcgga cgcggccgtg gtggctacga caaggacggt agaggcccca tgactggttc
     2041 cagcggaggt gaccgcggcg gattcaagaa cttcggtggc caccgcgact acggtccacg
     2101 tactgacgct gactccgaga gcgacaactc cgacaacaac accatcttcg tccagggact
     2161 gggagagggc gtgagcactg accaggtcgg cgaattcttc aagcagatcg gtatcatcaa
     2221 gaccaacaag aagactggca agccaatgat caacctgtac accgacaagg acactggcaa
     2281 gcctaagggc gaagctaccg tgtccttcga cgaccctccc agcgccaagg ctgccatcga
     2341 ctggttcgac ggcaaggagt tccacggaaa catcatcaag gtctctttcg ctactaggag
     2401 acctgaattc atgcgtggag gtggctcagg aggtggccgc cgtggaaggg gaggttacag
     2461 gggcagaggc ggattccagg gtcgtggtgg cgacccaaag tctggcgact gggtgtgccc
     2521 aaacccttca tgcggtaaca tgaacttcgc caggagaaac tcctgcaacc agtgcaacga
     2581 gcccaggcca gaagactcca gacctagcgg aggtgacttc cgcggacgtg gttacggcgg
     2641 agaaaggggc tacaggggaa gaggtggcag gggaggtgac agaggcggat acggtggcga
     2701 cagatctgga ggtggctacg gaggtgaccg ctcttcaggc ggaggttact ctggtgaccg
     2761 ttcaggcgga ggttacggcg gagaccgttc cggtggcgga tatggtggcg accgcggagg
     2821 tggctatgga ggtgaccgtg gcggaggtta tggcggagac aggggtggcg gatacggtgg
     2881 cgacagggga ggttatggcg gagacagagg tggcggatac ggtggcgacc gcggaggtta
     2941 tggcggagac cgtggtggct atggaggtga caggggcgga tatggtggcg acagaggagg
     3001 ttatggcgga gacaggtctc gtggtggcta cggaggtgac cgcggcggag gttctggtta
     3061 cggcggagac agaagcggtg gctatggagg tgacaggagc ggcggaggtt atggcggaga
     3121 ccgtggtggc ggatacggtg gcgacagggg aggttacgga ggaaagatgg gaggcagaaa
     3181 cgactaccgc aacgaccaaa ggaacagacc ctacggcgcg ccgggatcca gcagcggcag
     3241 agaaaacttg tatttccagg gcatggtgag caagggcgag gagctgttca ccggggtggt
     3301 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     3361 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     3421 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     3481 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     3541 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     3601 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     3661 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     3721 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     3781 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     3841 cgtgctgctg cccgacaacc actacctgag cacccagtcc aaactgagca aagaccccaa
     3901 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     3961 catggacgag ctgtacaagt aatagaagct tgtcgagaag tactagagga tcataatcag
     4021 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     4081 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     4141 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     4201 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct
     4261 tgagcctagg agatccgaac cagataagtg aaagcctgca tccgcagatc ttaattaata
     4321 aaacacgata cattgttatt agtacattta ttaagcgcta gattctgtgc gttgttgatt
     4381 tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt tagggtggta
     4441 tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa atattaaatc
     4501 ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt tttccgaacc
     4561 gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca aatcttgtag
     4621 cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag gttcgacgtc
     4681 gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt acaattgact
     4741 cgacgtaaac acgttaaata acgcttggac atatttaaca tcgggcgtgt tagctttatt
     4801 aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt
     4861 tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt
     4921 agttgagctt tttgggcctc atgggccttc cgctcactgc ccgctttcca gtcgggaaac
     4981 ctgtcgtgcc agctgcatta acatggtcat agctgtttcc ttgcgtattg ggcgctctcc
     5041 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggg taaagcctgg ggtgcctaat
     5101 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5161 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     5221 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     5281 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     5341 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5401 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5461 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5521 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5581 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5641 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5701 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5761 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5821 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5881 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5941 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6001 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagaac
     6061 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6121 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6181 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     6241 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     6301 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6361 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6421 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6481 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6541 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     6601 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6661 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6721 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6781 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6841 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6901 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     6961 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     7021 cgagataggg ttgagtggcc gctacagggc gctcccattc gccattcagg ctgcgcaact
     7081 gttgggaagg gcgtttcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     7141 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     7201 acgacggcca gtgagcgcga cgtaatacga ctcactatag ggcgaattgg cggaaggccg
     7261 tcaaggccgt gacgttaaaa ctattaagcc atccaatcga ccgttagtcg aatcaggacc
     7321 gctggtgcga gaagccgcga agtatggcga atgcatcgta taacgtgtgg agtccgctca
     7381 ttagagcgtc atgtttagac aagaaagcta catatttaat tgatcccgat gattttattg
     7441 ataaattgac cctaactcca tacacggtat tctacaatgg cggggttttg gtcaaaattt
     7501 ccggactgcg attgtacatg ctgttaacgg ctccgcccac tattaatgaa attaaaaatt
     7561 ccaattttaa aaaacgcagc aagagaaaca tttgtatgaa agaatgcgta gaaggaaaga
     7621 aaaatgtcgt ggacatgctg aacaacaaga ttaatatgcc tccgtgtata aaaaaaatat
     7681 tgaacgattt gaaagaaaac aatgtaccgc gcggcggtat gtacaggaag aggtttatac
     7741 taaactgtta cattgcaaac gtggtttcgt gtgccaagtg tgaaaaccga tgtttaatca
     7801 aggctctgac gcatttctac aaccacgact ccaagtgtgt gggtgaagtc atgcatcttt
     7861 taatcaaatc ccaagatgtg tataaaccac caaactgcca aaaaatgaaa actgtggaca
     7921 agctctgtcc gtttgctggc aactgcaagg gtctcaatcc tatttgtaat tattgaataa
     7981 taaaacaatt ataaatgcta aatttgtttt ttattaacga tacaaaccaa acgcaacaag
     8041 aacatttgta gtattatcta taattgaaaa cgcgtagtta taatcgctga ggtaatattt
     8101 aaaatcattt tcaaatgatt cacagttaat ttgcgacaat ataattttat tttcacataa
     8161 actagacgcc ttgtcgtctt cttcttcgta ttccttctct ttttcatttt tctcctcata
     8221 aaaattaaca tagttattat cgtatccata tatgtatcta tcgtatagag taaatttttt
     8281 gttgtcataa atatatatgt cttttttaat ggggtgtata gtaccgctgc gcatagtttt
     8341 tctgtaattt acaacagtgc tattttctgg tagttcttcg gagtgtgttg ctttaattat
     8401 taaatttata taatcaatga atttgggatc gtcggttttg tacaatatgt tgccggcata
     8461 gtacgcagct tcttctagtt caattacacc attttttagc agcaccggat taacataact
     8521 ttccaaaatg ttgtacgaac cgttaaacaa aaacagttca cctccctttt ctatactatt
     8581 gtctgcgagc agttgtttgt tgttaaaaat aacagccatt gtaatgagac gcacaaacta
     8641 atatcaca
//
