LOCUS       sequence-43897-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Rosonina E, Blencowe BJ
  TITLE     Analysis of the requirement for RNA polymerase II CTD heptapeptide 
            repeats in pre-mRNA splicing and 3'-end cleavage.
  JOURNAL   RNA. 2004 Apr;10(4):581-9.
  PUBMED    15037767
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2004-04"; volume: "10(4)"; pages: "581-9"
COMMENT     Sequence Label: FLAG-Pol-II 31-52
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             71..94
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      640..649
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagctggc
       61 taggaccatg gactacaagg acgacgatga caaggctagc atgcacgggg gtggcccccc
      121 ctcgggggac agcgcatgcc cgctgcgcac catcaagaga gtccagttcg gagtcctgag
      181 tccggatgaa ctgaagcgaa tgtctgtgac ggagggtggc atcaaatacc cagagacgac
      241 tgagggaggc cgccccaagc ttggggggct gatggacccg aggcaggggg tgattgagcg
      301 gactggccgc tgccaaacat gtgcaggaaa catgacagag tgtcctggcc actttggcca
      361 cattgaactg gccaagcctg tgtttcacgt gggcttcctg gtgaagacaa tgaaagtttt
      421 gcgctgtgtc tgcttcttct gctccaaact gcttgtggac tctaacaacc caaagatcaa
      481 ggatatcctg gctaagtcca agggacagcc caagaagcgg ctcacacatg tctacgacct
      541 ttgcaagggc aaaaacatat gcgagggtgg ggaggagatg gacaacaagt tcggtgtgga
      601 acaacctgag ggtgacgagg atctgaccaa agaaaagggc catggtggct gtgggcggta
      661 ccagcccagg atccggcgtt ctggcctaga gctgtatgcg gaatggaagc acgttaatga
      721 ggactctcag gagaagaaga tcctgctgag tccagagcga gtgcatgaga tcttcaaacg
      781 catctcagat gaggagtgtt ttgtgctggg catggagccc cgctatgcac ggccagagtg
      841 gatgattgtc acagtgctgc ctgtg
//
