LOCUS       sequence-439007-        4211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUPD2 for the Arabidopsis thaliana ABA-responsive MAPKKK18 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Calvache C, Vazquez-Vilar M, Moreno-Gimenez E, Orzaez D
  TITLE     A quantitative autonomous bioluminescence reporter system with a 
            wide dynamic range for Plant Synthetic Biology.
  JOURNAL   Plant Biotechnol J. 2024 Jan;22(1):37-47. doi: 10.1111/pbi.14146. 
            Epub 2023 Oct 26.
  PUBMED    37882352
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14146";
            journalName: "Plant Biotechnol J"; date: "2024-01"; volume: "22"; 
            issue: "1"; pages: "37-47"
COMMENT     Sequence Label: GB4666
FEATURES             Location/Qualifiers
     source          1..4211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(54..713)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     628..647
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     875..893
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(897..940)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    943..964
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      3127..3184
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(3208..3225)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3379..3967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3459..3478)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(join(4150..4211,1..33))
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
       61 ccgccctgcc actcgtcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
      121 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
      181 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
      241 aaatcaaaac tggtgaaact cacccaggga ttggctgata cgaaaaacat attctcaata
      301 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
      361 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gtgatgaaaa cgtttcagtt
      421 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
      481 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
      541 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
      601 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
      661 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
      721 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
      781 ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta
      841 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt
      901 cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct
      961 agagcgatga gtggtctcgg gagatagtag gtgttggtaa acaacggtga acgaactata
     1021 acttgatagt tacaaatgat atatttaaaa gtagatatca tagtgtttgt gataaaactt
     1081 ttttcaaaat gtaattcaaa caatttgaat tgagtaggtt gttgcttgaa gtaggcgttc
     1141 ctcattatac gaatcttttg cgtttctctt tgttttcatg ccacgtgttg tctctaccgc
     1201 tcacacgcca caagaacatt cttgaaaagg cttatcaaac aactttaaga acatatttct
     1261 attctaccca acagtaagaa aaataaaata tatcagttat ttggtttgac atagctagcg
     1321 tccaactaat tagaaatgat cagtttattt acattatcat ccaagaaaat gcattgattc
     1381 ataatgatta atacaaattt tcattggtta ttcatgggta cttttcttac cattaatgct
     1441 aaaaaataaa ataaaattca ccaatgtaca ataagtttct taattagcac acttttttgc
     1501 gtttggtcat ctgttttatg tttggtttta taagtatagc attacaatta ttattttagc
     1561 tttaaaaact taaaatgtaa attttaaaca cgtgaatgtg caagttatta tgcatgtggt
     1621 taataataaa gggtacatgt gcgtatgagc tgtgaatgtg cgtatatggc agtggacagc
     1681 cgtaaatgtc tgaccaaaat cgttttcctt atctttttac ccaacagtac tactactgcc
     1741 ttaggttaaa actcatcttg tcggacgctt cttcttacaa gtttacgtct tttatattct
     1801 aaaattgtaa caatatatta agtttgagaa atctttctta ttaattatta ctacgtcatt
     1861 tttatgtgtt aactattttt gtcgaagact tttttattct tgatcaatta gtccgaaaat
     1921 cactactaat tcgtaaacaa atttttatta ttaaatacct ctctgttttt cctcatttga
     1981 gaacgtgata taaaatcaac ataatcgaat cttcacagtt cacactagtt tatcattata
     2041 ataagtgagg ggattacatt ttaggtattt agagaaaacg gggaaaagat accaaaatat
     2101 cttagaggcc cgtggttcta tatgtacatt ttaattttta atttgctaaa gtcagccatg
     2161 aatactatag tatacctagt cagatttgat gatgatggtt gttaaaccca ccgctcctcc
     2221 tagtcaaaca tcttctacaa tttccaaaca cattcaaaaa acgcagaaaa aaaaaaaaaa
     2281 aatgtgtata tgtgttacaa ttcttaaaag aaaatgtaaa gccagataat tgttacaaga
     2341 gatgtacaaa gctccaagtg tgaagaacat caacatgaat atgatccatg aaatctacca
     2401 aatttccgtg cttgcacttt cccatacatt tttgtttttc acctaactct tacgaaccta
     2461 aaataaaata aaatacaaaa aaagggagaa agatttgagc acttgtcgac acatcgtagc
     2521 atcctacctc tgccgacacg tggctcgaga caaatacgga actcccggtc ggaacagaaa
     2581 caaatactta ttttgactac ctgaaattcg acacgtcatc accttctaga ctcaaaagag
     2641 tacaaaggcc aatgagactc taccattcct aacgaagtgt cacgtcactt cctatcaaac
     2701 ctttccttaa atacgttcac tgccgcgtca ctcctctttt ttggtcacgt ttcctatttt
     2761 tgtactaata cttcttcttc tctctacaca tattttgttg gtaatcacat cgaagattcg
     2821 aaattggatc acatttggta atcaactttt ttttccaaag tctccacgtg tatggacgat
     2881 tacatacgta cacttattta ttttcgcttt ggtggataaa ctcgatgtta ccaaaagtat
     2941 acactatcta attaccgaac caaaattaat ctgaaaccct tctatatata tatactttct
     3001 ttgcaataac agagtgaaag caaacattca aaaaaaaaaa aaaccatttt ttttttctta
     3061 ctcaaatctt ttttagtatc attctaatgt gagaccactc atcgcgacta gtagcggccg
     3121 ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
     3181 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3241 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3301 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3361 ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg
     3421 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3481 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3541 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3601 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3661 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3721 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3781 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3841 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3901 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3961 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4021 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4081 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc
     4141 gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     4201 cagatggagt t
//
