LOCUS       sequence-43901-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2-Flag-Kdrl-5p-FokI-EL
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             268..855
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 ggtacccgcc catatgcttg ccctgtcgag tcctgtgacc gccgcttcag ccagaagggg
       61 cacctcaccc gccatatccg gattcacaca ggccagaagc ccttccagtg tcgcatctgc
      121 atgaggaact tcagccgcag cgacgcgctc acccgccaca tccgcaccca cacaggcgag
      181 aagccttttg catgcgacat ctgcggcagg aagttcgcgc gcagcgacgc gctcaggaag
      241 cacaccaaaa tccatacagg tggatcccaa ctagtcaaaa gtgaactgga ggagaagaaa
      301 tctgaacttc gtcataaatt gaaatatgtg cctcatgaat atattgaatt aattgaaatt
      361 gccagaaatt ccactcagga tagaattctt gaaatgaagg taatggaatt ttttatgaaa
      421 gtttatggat atagaggtaa acatttgggt ggatcaagga aaccggacgg agcaatttat
      481 actgtcggat ctcctattga ttacggtgtg atcgtggata ctaaagctta tagcggaggt
      541 tataatctgc caattggcca agcagatgaa atggaacgat atgtcgaaga aaatcaaaca
      601 cgaaacaaac atctcaaccc taatgaatgg tggaaagtct atccatcttc tgtaacggaa
      661 tttaagtttt tatttgtgag tggtcacttt aaaggaaact acaaagctca gcttacacga
      721 ttaaatcata tcactaattg taatggagct gttcttagtg tagaagagct tttaattggt
      781 ggagaaatga ttaaagccgg cacattaacc ttagaggaag tgagacggaa atttaataac
      841 ggcgagataa acttttaatc taga
//
