LOCUS       sequence-439010-        9197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human integrin alpha5 full length.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9197)
  AUTHORS   Li J, Jo MH, Yan J, Hall T, Lee J, Lopez-Sanchez U, Yan S, Ha T, 
            Springer TA
  TITLE     Ligand binding initiates single-molecule integrin conformational 
            activation.
  JOURNAL   Cell. 2024 May 14:S0092-8674(24)00475-6. doi: 
            10.1016/j.cell.2024.04.049.
  PUBMED    38772370
REFERENCE   2  (bases 1 to 9197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            May 14:S0092-8674(24)00475-6. doi: 10.1016/j.cell.2024.04.049."
COMMENT     Sequence Label: pcDNA3.4 human alpha5 full
FEATURES             Location/Qualifiers
     source          1..9197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      117..545
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(204..223)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     414..435
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(554..574)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        559..888
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      739..874
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     801..820
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             955..1749
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1009..1028)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1619..1638
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1925..2046
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1962..1981)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2016..2035
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2095..2111)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2095..2111)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2108..2130)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2119..2135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2143..2149,2150..2167,2168..2173))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2188..2209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2326..2343)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2497..3085)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2577..2596)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3256..4047,4048..4116))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3879..3898
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4117..4221)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4296..4315)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4487..4866
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4867..5070
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5021..5041
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5067..5091
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             8250..8273
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             join(8283..8288,8289..8312,8313..8348,8349..8372)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     misc_feature    8411..8999
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8464..8484)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8882..8893)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9020..9039)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9064..9112
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
       61 ggcggcaggc cctgccatag cagatctgcg cagctggggc tctagggggt atccccacgc
      121 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      181 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      241 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      301 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      361 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
      421 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
      481 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
      541 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      601 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      661 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      721 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
      781 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
      841 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
      901 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
      961 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     1021 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     1081 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     1141 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     1201 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     1261 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     1321 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     1381 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     1441 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     1501 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     1561 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     1621 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     1681 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     1741 ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc ccaacctgcc
     1801 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     1861 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     1921 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     1981 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     2041 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     2101 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     2161 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     2221 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2281 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     2341 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2401 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2461 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2521 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2581 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2641 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2701 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2761 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2821 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2881 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2941 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3001 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3061 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3121 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3181 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3241 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3301 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3361 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3421 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3481 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3541 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3601 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3661 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3721 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3781 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3841 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3901 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3961 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4021 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4081 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4141 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4201 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     4261 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4321 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     4381 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     4441 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     4501 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     4561 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     4621 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     4681 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     4741 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     4801 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     4861 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     4921 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     4981 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     5041 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     5101 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctccggact
     5161 ctagagccac catgcctttg ctcctcctgt tgcccttgct ctgggctggc gccctcgcgt
     5221 tcaacttaga cgcggaggcc ccagcagtac tctcggggcc cccgggctcc ttcttcggat
     5281 tctcagtgga gttttaccgg ccgggaacag acggggtcag tgtgctggtg ggagcaccca
     5341 aggctaatac cagccagcca ggagtgctgc agggtggtgc tgtctacctc tgtccttggg
     5401 gtgccagccc cacacagtgc acccccattg aatttgacag caaaggctct cggctcctgg
     5461 agtcctcact gtccagctca gagggagagg agcctgtgga gtacaagtcc ttgcagtggt
     5521 tcggggcaac agttcgagcc catggctcct ccatcttggc atgcgctcca ctgtacagct
     5581 ggcgcacaga gaaggagcca ctgagcgacc ccgtgggcac ctgctacctc tccacagata
     5641 acttcacccg aattctggag tatgcaccct gccgctcaga tttcagctgg gcagcaggac
     5701 agggttactg ccaaggaggc ttcagtgccg agttcaccaa gactggccgt gtggttttag
     5761 gtggaccagg aagctatttc tggcaaggcc agatcctgtc tgccactcag gagcagattg
     5821 cagaatctta ttaccccgag tacctgatca acctggttca ggggcagctg cagactcgcc
     5881 aggccagttc catctatgat gacagctacc taggatactc tgtggctgtt ggtgaattca
     5941 gtggtgatga cacagaagac tttgttgctg gtgtgcccaa agggaacctc acttacggct
     6001 atgtcaccat ccttaatggc tcagacattc gatccctcta caacttctca ggggaacaga
     6061 tggcctccta ctttggctat gcagtggccg ccacagacgt caatggggac gggctggatg
     6121 acttgctggt gggggcaccc ctgctcatgg atcggacccc tgacgggcgg cctcaggagg
     6181 tgggcagggt ctacgtctac ctgcagcacc cagccggcat agagcccacg cccaccctta
     6241 ccctcactgg ccatgatgag tttggccgat ttggcagctc cttgaccccc ctgggggacc
     6301 tggaccagga tggctacaat gatgtggcca tcggggctcc ctttggtggg gagacccagc
     6361 agggagtagt gtttgtattt cctgggggcc caggagggct gggctctaag ccttcccagg
     6421 ttctgcagcc cctgtgggca gccagccaca ccccagactt ctttggctct gcccttcgag
     6481 gaggccgaga cctggatggc aatggatatc ctgatctgat tgtggggtcc tttggtgtgg
     6541 acaaggctgt ggtatacagg ggccgcccca tcgtgtccgc tagtgcctcc ctcaccatct
     6601 tccccgccat gttcaaccca gaggagcgga gctgcagctt agaggggaac cctgtggcct
     6661 gcatcaacct tagcttctgc ctcaatgctt ctggaaaaca cgttgctgac tccattggtt
     6721 tcacagtgga acttcagctg gactggcaga agcagaaggg aggggtacgg cgggcactgt
     6781 tcctggcctc caggcaggca accctgaccc agaccctgct catccagaat ggggctcgag
     6841 aggattgcag agagatgaag atctacctca ggaacgagtc agaatttcga gacaaactct
     6901 cgccgattca catcgctctc aacttctcct tggaccccca agccccagtg gacagccacg
     6961 gcctcaggcc agccctacat tatcagagca agagccggat agaggacaag gctcagatct
     7021 tgctggactg tggagaagac aacatctgtg tgcctgacct gcagctggaa gtgtttgggg
     7081 agcagaacca tgtgtacctg ggtgacaaga atgccctgaa cctcactttc catgcccaga
     7141 atgtgggtga gggtggcgcc tatgaggctg agcttcgggt caccgcccct ccagaggctg
     7201 agtactcagg actcgtcaga cacccaggga acttctccag cctgagctgt gactactttg
     7261 ccgtgaacca gagccgcctg ctggtgtgtg acctgggcaa ccccatgaag gcaggagcca
     7321 gtctgtgggg tggccttcgg tttacagtcc ctcatctccg ggacactaag aaaaccatcc
     7381 agtttgactt ccagatcctc agcaagaatc tcaacaactc gcaaagcgac gtggtttcct
     7441 ttcggctctc cgtggaggct caggcccagg tcaccctgaa cggtgtctcc aagcctgagg
     7501 cagtgctatt cccagtaagc gactggcatc cccgagacca gcctcagaag gaggaggacc
     7561 tgggacctgc tgtccaccat gtctatgagc tcatcaacca aggccccagc tccattagcc
     7621 agggtgtgct ggaactcagc tgtccccagg ctctggaagg tcagcagctc ctatatgtga
     7681 ccagagttac gggactcaac tgcaccacca atcaccccat taacccaaag ggcctggagt
     7741 tggatcccga gggttccctg caccaccagc aaaaacggga agctccaagc cgcagctctg
     7801 cttcctcggg acctcagatc ctgaaatgcc cggaggctga gtgtttcagg ctgcgctgtg
     7861 agctcgggcc cctgcaccaa caagagagcc aaagtctgca gttgcatttc cgagtctggg
     7921 ccaagacttt cttgcagcgg gagcaccagc catttagcct gcagtgtgag gctgtgtaca
     7981 aagccctgaa gatgccctac cgaatcctgc ctcggcagct gccccaaaaa gagcgtcagg
     8041 tggccacagc tgtgcaatgg accaaggcag aaggcagcta tggcgtccca ctgtggatca
     8101 tcatcctagc catcctgttt ggcctcctgc tcctaggtct actcatctac atcctctaca
     8161 agcttggatt cttcaaacgc tccctcccat atggcaccgc catggaaaaa gctcagctca
     8221 agcctccagc cacctctgat gccggtaccc ttgaggtgct tttccaaggt cccggttccg
     8281 gtagtgcatg gtctcatccc caatttgaaa aaggtggggg gtcaggcggc ggttcaggcg
     8341 ggagtgcttg gtcacacccc caatttgaga aataatgatc actcgaggat atcgcggccg
     8401 cgatctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     8461 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     8521 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     8581 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     8641 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     8701 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     8761 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     8821 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     8881 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     8941 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     9001 tggaacgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
     9061 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc
     9121 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     9181 tacgcccgcg tttcttc
//
