LOCUS       sequence-439020-       10446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human integrin alpha5 full length.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Li J, Jo MH, Yan J, Hall T, Lee J, Lopez-Sanchez U, Yan S, Ha T, 
            Springer TA
  TITLE     Ligand binding initiates single-molecule integrin conformational 
            activation.
  JOURNAL   Cell. 2024 May 14:S0092-8674(24)00475-6. doi: 
            10.1016/j.cell.2024.04.049.
  PUBMED    38772370
REFERENCE   2  (bases 1 to 10446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            May 14:S0092-8674(24)00475-6. doi: 10.1016/j.cell.2024.04.049."
COMMENT     Sequence Label: PD2529 human alpha5 full
FEATURES             Location/Qualifiers
     source          1..10446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    944..999
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(998..1017)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(1709..2297)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1789..1808)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2383..3192)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3106..3125
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(3211..3258)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             complement(3684..4283)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3767..3787)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4264..4283
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(4293..4792)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4341..4360)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     4734..4757
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     enhancer        4876..5179
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          5490..6506
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6514..6533
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 ttttcatagg tcacttacat atatcaatgg gtctgtttct gagctctact ctattttatc
       61 agcctcactg tctatcccca cacatctcat gctttgctct aaatcttgat atttagtgga
      121 acattctttc ccattttgtt ctacaagaat atttttgtta ttgtctttgg gctttctata
      181 tacattttga aatgaggttg acaagttaat aacaggccta ttgattggaa agtttgtcaa
      241 cgaattgtgg gtcttttggg gtttgctgcc ccttttacgc aatgtggata tcctgcttta
      301 atgcctttat atgcatgtat acaagcaaaa caggctttta ctttctcgcc aacttacaag
      361 gcctttctca gtaaacagta tatgaccctt taccccgttg ctcggcaacg gcctggtctg
      421 tgccaagtgt ttgctgacgc aacccccact ggttggggct tggccatagg ccatcagcgc
      481 atgcgtggaa cctttgtgtc tcctctgccg atccatactg cggaactcct agccgcttgt
      541 tttgctcgca gcaggtctgg agcaaacctc atcgggaccg acaattctgt cgtactctcc
      601 cgcaagtata catcgtttcc atggctgcta ggctgtgctg ccaactggat cctgcgcggg
      661 acgtcctttg tttacgtccc gtcggcgctg aatcccgcgg acgacccctc ccggggccgc
      721 ttggggctct accgcccgct tctccgtctg ccgtaccgtc cgaccacggg gcgcacctct
      781 ctttacgcgg actccccgtc tgtgccttct catctgccgg accgtgtgca cttcgcttca
      841 cctctgcacg tcgcatggag gccaccgtga acgcccaccg gaacctgccc aaggtcttgc
      901 ataagaggac tcttggactt tcagcaatgt catctcagtg gctaataaag gaaatttatt
      961 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     1021 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
     1081 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
     1141 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
     1201 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
     1261 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
     1321 agatctttgc atttttagac atttagaagc ctatatcttg ttacagaatt ggaattacac
     1381 aaaaattcta ccatattttg aaagcttagg ttgttctgaa aaaaacaata tattgttttc
     1441 ctgggtaaac taaaagtccc ctcgaggaaa ggcccctaaa gtgaaacagt gcaaaacgtt
     1501 caaaaactgt ctggcaatac aagttccact ttgaccaaaa cggctggcag taaaagggtt
     1561 aagaagactg tcagccttga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1621 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1681 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1741 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1801 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1861 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1921 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1981 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2041 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2101 gtgctacaga gttcttgaag tggtgggcta actacggcta cactagaaga acagtatttg
     2161 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2221 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2281 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2341 acgacgcgcg cgtaactcac gttaagggat tttggtcatg agttagaaaa actcatcgag
     2401 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     2461 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     2521 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     2581 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     2641 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     2701 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgaggcgaaa
     2761 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gagtgcaacc ggcgcaggaa
     2821 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     2881 cgctgttttt ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     2941 atgcttgatg gtcggaagtg gcataaattc cgtcagccag tttagtctga ccatctcatc
     3001 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     3061 cttcccatac aagcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     3121 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctcgacg tttcccgttg
     3181 gatatggctc attttttact tcctcacctt gtcgtattat actatgccga tatactatgc
     3241 cgatgattaa ttgtcgacac tgcgggggct ctgtgtggta agcaggtctt aaccttttta
     3301 ctgccaatga cgcatgggat acgtcgtggc agtaaaaggg cttaaatgcc aacgacgcgt
     3361 cccatacgtt gttggcattt taattcttct ctctgcagcg gcagcatgtg ccgccgctgc
     3421 agagagtttc tagcgatgac agcccctctg ggcaacgagc cgggggggct gtctttcttt
     3481 atgttttaaa tgcactgacc tcccacattc cctttttagt aaaatattca gaaataattt
     3541 aaatacatca ttgcaatgaa aataaatgtt ttttattagg cagaatccag atgctcaagg
     3601 cccttcataa tatcccccag tttagtagtt ggacttaggg aacaaaggaa cctttaatag
     3661 aaattggaca gcaagaaagc gagtcaggca ccgggcttgc gggtcatgca ccaggtgcgc
     3721 ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc cgagccgctc gtagaagggg
     3781 aggttgcggg gcgcggatgt ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc
     3841 actccgggga gcacgacggc gctgcccaga cccttgccct ggtggtcggg cgacacgccg
     3901 acggtggcca ggaaccacgc gggctccttg ggccggtgcg gcgccaggag gccttccatc
     3961 tgttgctgcg cggccagccg ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg
     4021 aacaccgccc ccgcttcgac gctctccggc gtggtccaga ccgccaccgc ggcgccgtcg
     4081 tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg tgaggaagag ttcttgcagc
     4141 tcggtgaccc gctcgatgtg gcggtccgga tcgacggtgt ggcgcgtggc ggggtagtcg
     4201 gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt cgtcgcgggt ggcgaggcgc
     4261 accgtgggct tgtactcggt catggtggcg gacgaaaggc ccggagatga ggaagaggag
     4321 aacagcgcgg cagacgtgcg cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt
     4381 cgggcgcccg ccccgcccct gagcccgccc ctgagcccgc ccccggaccc accccttccc
     4441 agcctctgag cccagaaagc gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct
     4501 acccgcttcc attgctcagc ggtgctgtcc atctgcacga gactagtgag tcgtgctact
     4561 tccatttgtc acgtcctgca cgacgcgagc tgcggggcgg gggggaactt cctgactagg
     4621 ggaggagtag aaggtggcgc gaaggggcca ccaaagaacg gagccggttg gcgcctaccg
     4681 gtggatgtgg aatgtgtgcg aggccagagg ccacttgtgt agcgccaagt gcccagcggg
     4741 gctgctaaag cgcatgctcc agactgcctt gggaaaagcg cctcccctac ccggtagaga
     4801 aacttgatct gtcgccgcaa ttcaaacttc gtgaggctcc ggtgcccgtc agtgacctgc
     4861 tatactctgg agacgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     4921 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     4981 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     5041 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     5101 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     5161 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
     5221 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
     5281 gggggcgggg gggggggggg cgcgcgccag gcgggggggg gggggggggg gggcgcgcgc
     5341 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
     5401 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
     5461 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc
     5521 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
     5581 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
     5641 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
     5701 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
     5761 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
     5821 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
     5881 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
     5941 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     6001 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     6061 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     6121 aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt
     6181 gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg
     6241 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg
     6301 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
     6361 ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg
     6421 gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg
     6481 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
     6541 atcattttgg caaagaattc ggcttgatcg aagccgtctc aggggagaga ccacacccaa
     6601 gctgtctaga gccgccacct tgtgaagagc gccgccacca tgcctttgct cctcctgttg
     6661 cccttgctct gggctggcgc cctcgcgttc aacttagacg cggaggcccc agcagtactc
     6721 tcggggcccc cgggctcctt cttcggattc tcagtggagt tttaccggcc gggaacagac
     6781 ggggtcagtg tgctggtggg agcacccaag gctaatacca gccagccagg agtgctgcag
     6841 ggtggtgctg tctacctctg tccttggggt gccagcccca cacagtgcac ccccattgaa
     6901 tttgacagca aaggctctcg gctcctggag tcctcactgt ccagctcaga gggagaggag
     6961 cctgtggagt acaagtcctt gcagtggttc ggggcaacag ttcgagccca tggctcctcc
     7021 atcttggcat gcgctccact gtacagctgg cgcacagaga aggagccact gagcgacccc
     7081 gtgggcacct gctacctctc cacagataac ttcacccgaa ttctggagta tgcaccctgc
     7141 cgctcagatt tcagctgggc agcaggacag ggttactgcc aaggaggctt cagtgccgag
     7201 ttcaccaaga ctggccgtgt ggttttaggt ggaccaggaa gctatttctg gcaaggccag
     7261 atcctgtctg ccactcagga gcagattgca gaatcttatt accccgagta cctgatcaac
     7321 ctggttcagg ggcagctgca gactcgccag gccagttcca tctatgatga cagctaccta
     7381 ggatactctg tggctgttgg tgaattcagt ggtgatgaca cagaagactt tgttgctggt
     7441 gtgcccaaag ggaacctcac ttacggctat gtcaccatcc ttaatggctc agacattcga
     7501 tccctctaca acttctcagg ggaacagatg gcctcctact ttggctatgc agtggccgcc
     7561 acagacgtca atggggacgg gctggatgac ttgctggtgg gggcacccct gctcatggat
     7621 cggacccctg acgggcggcc tcaggaggtg ggcagggtct acgtctacct gcagcaccca
     7681 gccggcatag agcccacgcc cacccttacc ctcactggcc atgatgagtt tggccgattt
     7741 ggcagctcct tgacccccct gggggacctg gaccaggatg gctacaatga tgtggccatc
     7801 ggggctccct ttggtgggga gacccagcag ggagtagtgt ttgtatttcc tgggggccca
     7861 ggagggctgg gctctaagcc ttcccaggtt ctgcagcccc tgtgggcagc cagccacacc
     7921 ccagacttct ttggctctgc ccttcgagga ggccgagacc tggatggcaa tggatatcct
     7981 gatctgattg tggggtcctt tggtgtggac aaggctgtgg tatacagggg ccgccccatc
     8041 gtgtccgcta gtgcctccct caccatcttc cccgccatgt tcaacccaga ggagcggagc
     8101 tgcagcttag aggggaaccc tgtggcctgc atcaacctta gcttctgcct caatgcttct
     8161 ggaaaacacg ttgctgactc cattggtttc acagtggaac ttcagctgga ctggcagaag
     8221 cagaagggag gggtacggcg ggcactgttc ctggcctcca ggcaggcaac cctgacccag
     8281 accctgctca tccagaatgg ggctcgagag gattgcagag agatgaagat ctacctcagg
     8341 aacgagtcag aatttcgaga caaactctcg ccgattcaca tcgctctcaa cttctccttg
     8401 gacccccaag ccccagtgga cagccacggc ctcaggccag ccctacatta tcagagcaag
     8461 agccggatag aggacaaggc tcagatcttg ctggactgtg gagaagacaa catctgtgtg
     8521 cctgacctgc agctggaagt gtttggggag cagaaccatg tgtacctggg tgacaagaat
     8581 gccctgaacc tcactttcca tgcccagaat gtgggtgagg gtggcgccta tgaggctgag
     8641 cttcgggtca ccgcccctcc agaggctgag tactcaggac tcgtcagaca cccagggaac
     8701 ttctccagcc tgagctgtga ctactttgcc gtgaaccaga gccgcctgct ggtgtgtgac
     8761 ctgggcaacc ccatgaaggc aggagccagt ctgtggggtg gccttcggtt tacagtccct
     8821 catctccggg acactaagaa aaccatccag tttgacttcc agatcctcag caagaatctc
     8881 aacaactcgc aaagcgacgt ggtttccttt cggctctccg tggaggctca ggcccaggtc
     8941 accctgaacg gtgtctccaa gcctgaggca gtgctattcc cagtaagcga ctggcatccc
     9001 cgagaccagc ctcagaagga ggaggacctg ggacctgctg tccaccatgt ctatgagctc
     9061 atcaaccaag gccccagctc cattagccag ggtgtgctgg aactcagctg tccccaggct
     9121 ctggaaggtc agcagctcct atatgtgacc agagttacgg gactcaactg caccaccaat
     9181 caccccatta acccaaaggg cctggagttg gatcccgagg gttccctgca ccaccagcaa
     9241 aaacgggaag ctccaagccg cagctctgct tcctcgggac ctcagatcct gaaatgcccg
     9301 gaggctgagt gtttcaggct gcgctgtgag ctcgggcccc tgcaccaaca agagagccaa
     9361 agtctgcagt tgcatttccg agtctgggcc aagactttct tgcagcggga gcaccagcca
     9421 tttagcctgc agtgtgaggc tgtgtacaaa gccctgaaga tgccctaccg aatcctgcct
     9481 cggcagctgc cccaaaaaga gcgtcaggtg gccacagctg tgcaatggac caaggcagaa
     9541 ggcagctatg gcgtcccact gtggatcatc atcctagcca tcctgtttgg cctcctgctc
     9601 ctaggtctac tcatctacat cctctacaag cttggattct tcaaacgctc cctcccatat
     9661 ggcaccgcca tggaaaaagc tcagctcaag cctccagcca cctctgatgc ctgataaggg
     9721 ttctttaaac gccaatacaa gtcaatcctt caagaagaaa atagaagaga ctcttggtcc
     9781 tatatcaaca gcaagtcaaa cgacgattga taagctagcg gttgaggtct ctaaaagcgt
     9841 cttcctgttc tcatcacatc atatcaaggt tatataccat caatattgcc acagatgtta
     9901 cttagccttt taatatttct ctaatttagt gtatatgcaa tgatagttct ctgatttctg
     9961 agattgagtt tctcatgtgt aatgattatt tagagtttct ctttcatctg ttcaaatttt
    10021 tgtctagttt tattttttac tgatttgtaa gacttctttt tataatctgc atattacaat
    10081 tctctttact ggggtgttgc aaatattttc tgtcattcta tggcctgact tttcttaatg
    10141 gttttttaat tttaaaaata agtcttaata ttcatgcaat ctaattaaca atcttttctt
    10201 tgtggttagg actttgagtc ataagaaatt tttctctaca ctgaagtcat gatggcatgc
    10261 ttctatatta ttttctaaaa gatttaaagt tttgccttct ccatttagac ttataattca
    10321 ctggaatttt tttgtgtgta tggtatgaca tatgggttcc cttttatttt ttacatataa
    10381 atatatttcc ctgtttttct aaaaaagaaa aagatcatca ttttcccatt gtaaaatgcc
    10441 atattt
//
