LOCUS       sequence-439055-        9775 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human integrin beta1 Q280* K418* 
            full-length.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9775)
  AUTHORS   Li J, Jo MH, Yan J, Hall T, Lee J, Lopez-Sanchez U, Yan S, Ha T, 
            Springer TA
  TITLE     Ligand binding initiates single-molecule integrin conformational 
            activation.
  JOURNAL   Cell. 2024 May 14:S0092-8674(24)00475-6. doi: 
            10.1016/j.cell.2024.04.049.
  PUBMED    38772370
REFERENCE   2  (bases 1 to 9775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            May 14:S0092-8674(24)00475-6. doi: 10.1016/j.cell.2024.04.049."
COMMENT     Sequence Label: Beta1 Q280* K418* full-length
FEATURES             Location/Qualifiers
     source          1..9775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(482..1070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(562..581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1156..1965)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1879..1898
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1984..2031)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             complement(2457..3056)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2540..2560)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3037..3056
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3066..3565)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3114..3133)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3507..3530
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     enhancer        3649..3952
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3954..4228
                     /label=chicken beta-actin promoter
     intron          4229..5245
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     5169..5191
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     5253..5272
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             7747..7773
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7780..7824
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     polyA_signal    9492..9547
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9546..9565)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
       61 ctcccagtca tagctgtccc tcttctctta tggagatctt tgcattttta gacatttaga
      121 agcctatatc ttgttacaga attggaatta cacaaaaatt ctaccatatt ttgaaagctt
      181 aggttgttct gaaaaaaaca atatattgtt ttcctgggta aactaaaagt cccctcgagg
      241 aaaggcccct aaagtgaaac agtgcaaaac gttcaaaaac tgtctggcaa tacaagttcc
      301 actttgacca aaacggctgg cagtaaaagg gttaagaaga ctgtcagcct tgagcggtat
      361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggg
      901 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1081 tgatcttttc tacggggtct gacgctcagt ggaacgacgc gcgcgtaact cacgttaagg
     1141 gattttggtc atgagttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
     1201 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     1261 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     1321 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     1381 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac
     1441 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     1501 attcattcgt gattgcgcct gagcgaggcg aaatacgcga tcgctgttaa aaggacaatt
     1561 acaaacagga atcgagtgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     1621 acctgaatca ggatattctt ctaatacctg gaacgctgtt tttccgggga tcgcagtggt
     1681 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gtggcataaa
     1741 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     1801 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaagcgat agattgtcgc
     1861 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     1921 ggaatttaat cgcggcctcg acgtttcccg ttggatatgg ctcatttttt acttcctcac
     1981 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcga cactgcgggg
     2041 gctctgtgtg gtaagcaggt cttaaccttt ttactgccaa tgacgcatgg gatacgtcgt
     2101 ggcagtaaaa gggcttaaat gccaacgacg cgtcccatac gttgttggca ttttaattct
     2161 tctctctgca gcggcagcat gtgccgccgc tgcagagagt ttctagcgat gacagcccct
     2221 ctgggcaacg agccgggggg gctgtctttc tttatgtttt aaatgcactg acctcccaca
     2281 ttcccttttt agtaaaatat tcagaaataa tttaaataca tcattgcaat gaaaataaat
     2341 gttttttatt aggcagaatc cagatgctca aggcccttca taatatcccc cagtttagta
     2401 gttggactta gggaacaaag gaacctttaa tagaaattgg acagcaagaa agcgagtcag
     2461 gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc gacgtcggcg
     2521 gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga tgtctccagg
     2581 aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac ggcgctgccc
     2641 agacccttgc cctggtggtc gggcgacacg ccgacggtgg ccaggaacca cgcgggctcc
     2701 ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag ccgggaaccg
     2761 ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc gacgctctcc
     2821 ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt gccgatgtcg
     2881 agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat gtggcggtcc
     2941 ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag ggtgcgtacg
     3001 gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc ggtcatggtg
     3061 gcggacgaaa ggcccggaga tgaggaagag gagaacagcg cggcagacgt gcgcttttga
     3121 agcgtgcaga atgccgggcc tccggaggac cttcgggcgc ccgccccgcc cctgagcccg
     3181 cccctgagcc cgcccccgga cccacccctt cccagcctct gagcccagaa agcgaaggag
     3241 caaagctgct attggccgct gccccaaagg cctacccgct tccattgctc agcggtgctg
     3301 tccatctgca cgagactagt gagtcgtgct acttccattt gtcacgtcct gcacgacgcg
     3361 agctgcgggg cgggggggaa cttcctgact aggggaggag tagaaggtgg cgcgaagggg
     3421 ccaccaaaga acggagccgg ttggcgccta ccggtggatg tggaatgtgt gcgaggccag
     3481 aggccacttg tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc tccagactgc
     3541 cttgggaaaa gcgcctcccc tacccggtag agaaacttga tctgtcgccg caattcaaac
     3601 ttcgtgaggc tccggtgccc gtcagtgacc tgctatactc tggagacgcg ttacataact
     3661 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     3721 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     3781 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     3841 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     3901 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
     3961 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
     4021 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg ggcgcgcgcc
     4081 aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc
     4141 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
     4201 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
     4261 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
     4321 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
     4381 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     4441 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     4501 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     4561 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     4621 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     4681 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     4741 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     4801 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
     4861 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     4921 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
     4981 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
     5041 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     5101 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
     5161 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     5221 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     5281 tcattttggc aaagaattcg gcttgatcga agccgtctca ggggagagac cacacccaag
     5341 ctgtctagag ccgccaccat gcctttgctc ctcctgttgc ccttgctctg ggctggcgcc
     5401 ctcgcgcaga cggacgagaa tcgatgtttg aaagcgaatg caaaaagctg cggagagtgc
     5461 attcaggccg gaccgaattg tggatggtgt acaaattcga catttttgca agagggaatg
     5521 cccacgtcgg cccgatgtga tgacctcgag gccttgaaaa agaaaggatg tccaccggat
     5581 gacatcgaga acccacgggg ctcaaaggac atcaaaaaga acaagaatgt cacaaaccgc
     5641 tcgaagggaa ctgctgaaaa gcttaaaccg gaggacatca cgcagatcca gccccaacag
     5701 ctggtgctcc gattgcggtc cggtgagcca cagacgttta ctctcaagtt caagagagcg
     5761 gaggattacc cgatcgatct ttactatctt atggaccttt cgtactccat gaaggatgat
     5821 ttggaaaatg tcaagagcct cggtactgac ttgatgaatg aaatgcgccg cattaccagc
     5881 gatttccgaa tcggattcgg atcatttgtg gaaaagaccg tcatgcccta catttcgacc
     5941 actccggcaa agttgcgcaa cccgtgcacc tcagaacaga actgcacctc gcctttttcg
     6001 tataagaacg tcctcagctt gacaaacaag ggtgaagtgt tcaatgagct ggtcggaaag
     6061 caaagaatct cgggaaacct cgatagcccg gagggagggt ttgatgcaat catgcaagtg
     6121 gcggtatgcg ggtcccttat tggctggaga aacgtgacca gactcctggt gttttccact
     6181 gatgcgggat ttcatttcgc cggagacggg aaactgggag ggattgtact tccgaatgac
     6241 ggatagtgtc atttggagaa caacatgtat acgatgtccc actactatga ctacccctcg
     6301 atcgcgcacc tcgtccaaaa actgagcgaa aacaatatcc agactatctt tgcggtcacg
     6361 gaggagtttc agcccgtgta taaagaactg aaaaacttga tccctaagtc cgcagtcgga
     6421 acgctgtcgg ccaacagctc gaatgtgatc cagttgatca tcgatgcgta taactcactt
     6481 tcatcagagg tcatccttga gaacggaaag cttagcgaag gagtgacaat ctcgtacaaa
     6541 tcatattgca agaatggagt gaatggcacc ggcgagaatg gccggaaatg ttcaaacatt
     6601 tccatcggcg acgaggtcca gtttgagatc agcattactt ccaataagtg ccctaagtag
     6661 gactcggact cattcaaaat tcggcctctc gggtttaccg aagaggtaga ggtgatcctc
     6721 cagtacattt gtgaatgcga gtgtcaatca gagggtatcc ccgaaagccc caagtgtcat
     6781 gagggtaacg gaacgttcga atgcggggca tgtagatgta acgaaggtcg cgtcggaagg
     6841 cactgcgaat gctcgactga tgaggtaaac tcggaagaca tggatgcgta ttgccgaaaa
     6901 gagaattcga gcgaaatctg ctcaaacaac ggggaatgtg tgtgcggaca atgtgtctgc
     6961 cggaaacggg ataacacaaa tgagatctac tccggaaagt tttgcgaatg cgacaatttc
     7021 aactgtgaca ggtccaacgg tcttatctgt ggtggtaatg gagtctgcaa gtgccgcgtc
     7081 tgcgagtgta atccgaatta cacaggctca gcgtgtgatt gcagcctcga cacgtcaaca
     7141 tgcgaggcgt ccaacgggca gatttgtaac gggcgaggga tttgcgagtg tggggtgtgc
     7201 aaatgcactg accccaaatt ccaggggcag acgtgcgaga tgtgccagac gtgtcttggt
     7261 gtctgtgcgg agcataagga atgcgtgcag tgcagagcgt tcaacaaagg ggaaaagaaa
     7321 gatacgtgta cgcaagaatg ttcgtatttc aatatcacga aggtggagtc gagggataag
     7381 ctcccgcagc cagtacagcc ggaccccgtc agccattgca aggaaaagga cgtggatgat
     7441 tgctggttct atttcacata ctccgtgaat ggtaataatg aggtaatggt acatgtcgtg
     7501 gagaaccccg aatgccccac gggtcccgac atcattccca tagtagctgg agtcgtggca
     7561 ggtatcgtgc ttatcggtct ggcgttgttg ttgatctgga aactcttgat gatcatccat
     7621 gaccgcagag agtttgccaa gtttgagaaa gagaagatga atgcaaaatg ggacactggc
     7681 gaaaacccaa tatacaaaag cgctgttaca acagtggtga accccaagta cgagggcaaa
     7741 gctagctacc catacgatgt tccagattac gctggcagcg ggttgaatga tattttcgag
     7801 gctcagaaga tagaatggca tgaatgataa tcgtggaaca gtacgaacgc gccgagggcc
     7861 gccactccac cggcggcatg gacgagctgt acaagtgata aagtacttga taagctcttc
     7921 aggttgaggt ctctaaaagc gtcttcctgt tctcatcaca tcatatcaag gttatatacc
     7981 atcaatattg ccacagatgt tacttagcct tttaatattt ctctaattta gtgtatatgc
     8041 aatgatagtt ctctgatttc tgagattgag tttctcatgt gtaatgatta tttagagttt
     8101 ctctttcatc tgttcaaatt tttgtctagt tttatttttt actgatttgt aagacttctt
     8161 tttataatct gcatattaca attctcttta ctggggtgtt gcaaatattt tctgtcattc
     8221 tatggcctga cttttcttaa tggtttttta attttaaaaa taagtcttaa tattcatgca
     8281 atctaattaa caatcttttc tttgtggtta ggactttgag tcataagaaa tttttctcta
     8341 cactgaagtc atgatggcat gcttctatat tattttctaa aagatttaaa gttttgcctt
     8401 ctccatttag acttataatt cactggaatt tttttgtgtg tatggtatga catatgggtt
     8461 cccttttatt ttttacatat aaatatattt ccctgttttt ctaaaaaaga aaaagatcat
     8521 cattttccca ttgtaaaatg ccatattttt ttcataggtc acttacatat atcaatgggt
     8581 ctgtttctga gctctactct attttatcag cctcactgtc tatccccaca catctcatgc
     8641 tttgctctaa atcttgatat ttagtggaac attctttccc attttgttct acaagaatat
     8701 ttttgttatt gtctttgggc tttctatata cattttgaaa tgaggttgac aagttaataa
     8761 caggcctatt gattggaaag tttgtcaacg aattgtgggt cttttggggt ttgctgcccc
     8821 ttttacgcaa tgtggatatc ctgctttaat gcctttatat gcatgtatac aagcaaaaca
     8881 ggcttttact ttctcgccaa cttacaaggc ctttctcagt aaacagtata tgacccttta
     8941 ccccgttgct cggcaacggc ctggtctgtg ccaagtgttt gctgacgcaa cccccactgg
     9001 ttggggcttg gccataggcc atcagcgcat gcgtggaacc tttgtgtctc ctctgccgat
     9061 ccatactgcg gaactcctag ccgcttgttt tgctcgcagc aggtctggag caaacctcat
     9121 cgggaccgac aattctgtcg tactctcccg caagtataca tcgtttccat ggctgctagg
     9181 ctgtgctgcc aactggatcc tgcgcgggac gtcctttgtt tacgtcccgt cggcgctgaa
     9241 tcccgcggac gacccctccc ggggccgctt ggggctctac cgcccgcttc tccgtctgcc
     9301 gtaccgtccg accacggggc gcacctctct ttacgcggac tccccgtctg tgccttctca
     9361 tctgccggac cgtgtgcact tcgcttcacc tctgcacgtc gcatggaggc caccgtgaac
     9421 gcccaccgga acctgcccaa ggtcttgcat aagaggactc ttggactttc agcaatgtca
     9481 tctcagtggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     9541 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     9601 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     9661 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     9721 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt cttta
//
