LOCUS       sequence-439073-        5920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector with PabstBR promoter, hygR marker, and replicates
            in A. baumannii and E. coli; empty vector plasmid with MCS for 
            cloning - we recommend NcoI/BamHI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Bacon EE, Tran JS, Nadig N, Peters JM
  TITLE     Modular, inducible, and titratable expression systems for 
            Escherichia coli and Acinetobacter baumannii.
  JOURNAL   Microbiol Spectr. 2024 Nov 5;12(11):e0130624. doi: 
            10.1128/spectrum.01306-24. Epub 2024 Sep 20.
  PUBMED    39302127
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr."; date: "2024-11-5"; volume: "12(11):e0130624. doi"; pages: 
            " 10.1128/spectrum.01306-24. Epub 2024 Sep 20"
COMMENT     Sequence Label: pJMP3665
FEATURES             Location/Qualifiers
     source          1..5920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(841..846,847..863,864..869))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     terminator      complement(985..1012)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1104..1190)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1343..1360
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     misc_feature    1393..1449
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             complement(1669..2751)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2713..2732
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(2752..2829)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     rep_origin      complement(2915..3460)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2995..3014)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            3843..3952
                     /note="incP origin of transfer"
ORIGIN
        1 gcggttcgcg acctgccagc agggtatcaa catccggcag ccaatcttcc agacggtcca
       61 gcagacgcgg actcagataa ccccaaccac gatgatcttc aacggttgcg gcacgacgtt
      121 cacgcagcag ttccggaaaa acttcgctat gcggtgtcag aacggtatta ccggtcagcg
      181 gaacacgatg cagacgaccc agaacacgtc ccagttcacg tgccagtgcc agcagtgcat
      241 tacgatcggt ggtgccatcc attgcgctac gccaggtggt gccggtcata cggctcataa
      301 ccagatacgg ccacggccat gcacctgtac caggacgcag ttcaccacga cctaacagac
      361 gaggaaccgg aaccggtgca tctgccagaa ctgcataggc ttcgctttcg cttgccaggc
      421 tttccggacc acaccaatgt tcaccaaaca gtttaataac aggatcaggt tcaccaacca
      481 gaaccggatt ggtgctttca cccggaacac gcagaacagg cggaaccggc agacccagtt
      541 cttccagtgc acgacgtgcc agcggttccc aaaattcttg atcattgcgc aggcttgcat
      601 agctatcatc actatcaata cgatccagca gcagcaggct ttcttgggtc atcgttgctg
      661 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
      721 atagactgta cgaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
      781 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac
      841 agtttacgaa ccgaacaggc ttatgtcaac tgggttcgtg ccttcatccg tttccacggt
      901 gtgcgtccat gagcaaatat tatacgcaag gcgacaaggc tcgagagttc aacagacagc
      961 cttcgttctt atggccgttc aggaaaaagg ccatccgtca ggatggcctt ctgcttaatt
     1021 tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcgca
     1081 acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     1141 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggca
     1201 gttccctact ctcgcatggg gagaccccac actaccatcg gcgctacggc gtttcacttc
     1261 tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccaggca aattctgttt
     1321 tatcagaccg cttctgcgtt ctgatttaat ctgtatcagg ctgaaaatct tctctcatcc
     1381 gccaaaacag ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag
     1441 ctcgaattcc atggtctgtt tcctgtgtgc tgctgatgtg ctcagtatct tgttatccgc
     1501 tcacattgtc aatattttat ctgattaatg agacgtcttt acgacctgca cagccatacc
     1561 acagcttccg atggctgcct gacgccagaa gcattggtgc accgtgcagt cgatgataag
     1621 ctgtcaaacc agatcaattc gcgctaactt acattaattg cgttgcgctc actgcccgct
     1681 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1741 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
     1801 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
     1861 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
     1921 ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt
     1981 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
     2041 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
     2101 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
     2161 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
     2221 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
     2281 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
     2341 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
     2401 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
     2461 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
     2521 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
     2581 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     2641 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     2701 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     2761 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcaccattc
     2821 gatggtgtca acgtaaatgc atgccgcttc gccttccgtt tgggcctaga attccggttt
     2881 aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca
     2941 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat
     3001 accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
     3061 ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc
     3121 gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc
     3181 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca
     3241 ctggcagcag ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta
     3301 aggctaaact gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt
     3361 caaagagttg gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt
     3421 ttcagagcaa gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaatca
     3481 gataaaatat ttctagctag agtcgatctt cgccagcagg gcgaggatcg tggcatcacc
     3541 gaaccgcgcc gtgcgcgggt cgtcggtgag ccagagtttc agcaggccgc ccaggcggcc
     3601 caggtcgcca ttgatgcggg ccagctcgcg gacgtgctca tagtccacga cgcccgtgat
     3661 tttgtagccc tggccgacgg ccagcaggta ggccgacagg ctcatgccgg ccgccgccgc
     3721 cttttcctca atcgctcttc gttcgtctgg aaggcagtac accttgatag gtgggctgcc
     3781 cttcctggtt ggcttggttt catcagccat ccgcttgccc tcatctgtta cgccggcggt
     3841 agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac
     3901 agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg
     3961 ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc
     4021 gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc
     4081 ggaaaagcgc tgcttccctg ctgttttgtg cgcgcctgag gacataaggg ctgcaagcca
     4141 agatcgtaga aatatctatg attatcttga agaacgcaac cctatagcag ctattgaaat
     4201 tgatgattta attgaagaaa agacagattt acttgttgat aatcgactga tggggcgcac
     4261 aggcagacag aaagatacta gggagttagt gatacatccg cattatgtgg ttgtatatga
     4321 catcactgat ataatacgga tactcagagt gctacacaca tcgcaggagt ggtcatgact
     4381 tactcatgta ctttggatta tttagtgtta taaaatcctg atttataaat tttttttgtt
     4441 aaaaaagata aaagcccctt gcaattgctt ggggctttac cgtaatttat ggggtacaga
     4501 tcttcgatac tgacatatcg gcaatcgaaa gcattaaggt ttgacgaccg ctaatgattt
     4561 caccacaggg gcttaatgta cctgtcttaa attctaaggt tttaactcgc tttgtcaagc
     4621 atagacccca aaaatttagc caatgtctgt aactcaatct gtccatgtgt gggtgatgag
     4681 gtacagtgac gctagcacac atcggaaaaa cgctattact aggggaactg aacagagtag
     4741 cggacgcaat gagtagtcat ttaattggcg gttatgagcg tgttcaggcg gtgctatcaa
     4801 tcgtaatcat aacagtggca gcttgataca gtgatgtcat ccctgatgcg aaagcgaccg
     4861 accgacggta catcgaatgg gaatacttta gggtgatttt taagaatcgc tctagggtga
     4921 gtatttccca ttcagctctg ctccctccct ctggtacttt aatcaaaagc actactaaac
     4981 atatgttttt aaataaaaaa tattgatata gagataatat tagtaagaat aattaaacaa
     5041 ttgaatatag ataaatcatt gttaaataaa gattaattat taaaatgaat gcatacttat
     5101 atataaatca atgatttaaa atatttgata aagaaaactt ttcaaaaaaa atataattga
     5161 gattgtgtca tttcggtcaa ttcttaatat gttccacgca agttttagct atggtgctaa
     5221 acagaaattt gctgaaaaag aacttttcac tgaactggtt aaaatgtaag cagcctgaga
     5281 gccgccaaaa attttaaaaa caaaccgcct taatcatctt caaaaaatac ctctaaaacc
     5341 tcaccatttg cgttttaaga cccatatttc atcctgccct tatgttccca tgctgatagc
     5401 tataaagtgt ctgtaatcgc ttcctatgac gttctaggct gttgataact tttggaacaa
     5461 cgcaaaatgt taaaatcctg ctgtgataat gtcaacggac tagtccagaa accgcagtac
     5521 atcggagcgc ttttgaagct gatgctcgag atctcggctt gaacgaattg ttattatgca
     5581 cccggtgcgg tatcaggcgg accccacaga aactgtgcta attcttccgg atcggtaaaa
     5641 ccgctcagat ccagcggtgt ttcttcaaac acttcaaaat catgcagaaa ggtaaaggcc
     5701 agcagttcac gggcaaaatc ttcggtacgt ttccactgtg caccatccag cagggctgcc
     5761 agaatttcac gatcaccacg aaatgcattc agatgcagct gaaccaggct ataacggcta
     5821 tcaccggcat aaacatcggt gaaatcaaca ataccggtaa cttcggttgc tgccagatca
     5881 acaaaaatgt tggtgccatg cagatcacca tgaacaaaac
//
