LOCUS       sequence-439112-       10979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rho reporter, sensor dead.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10979)
  AUTHORS   Marshall-Burghardt S, Migueles-Ramirez RA, Lin Q, El Baba N, Saada 
            R, Umar M, Mavalwala K, Hayer A
  TITLE     Excitable Rho dynamics control cell shape and motility by 
            sequentially activating ERM proteins and actomyosin contractility.
  JOURNAL   Sci Adv. 2024 Sep 6;10(36):eadn6858. doi: 10.1126/sciadv.adn6858. 
            Epub 2024 Sep 6.
  PUBMED    39241071
REFERENCE   2  (bases 1 to 10979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-09-6"; volume: "10(36):eadn6858. doi"; pages: " 
            10.1126/sciadv.adn6858. Epub 2024 Sep 6"
COMMENT     Sequence Label: pLV-RhoB Sensor (PKN-L59Q)-IRES-Blast
FEATURES             Location/Qualifiers
     source          1..10979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             331..588
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    635..760
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1257..1490
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1675..1719
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1982..2097
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2175..2386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2387..3325
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3282..3302
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3347..3364
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3650..4381
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="DGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGSGSGSVSKGEELFTGVVPILVELDGDVNGHKFSVSGE
                     GEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEG
                     YVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNV
                     YITADKQKNGIKANFKIRHNIE"
     primer_bind     3784..3805
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3908..3929)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4169..4188)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    5943..6492
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6087..6104)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6314..6333
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    6947..7535
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7000..7020)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7418..7429)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7742..8375
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8503..8519)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8503..8519)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8516..8538)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8527..8543
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8551..8557,8558..8575,8576..8581))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8596..8617
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8734..8751)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8905..9493)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8985..9004)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9664..10455,10456..10524))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10287..10306
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10525..10629)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10677..10811
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10714..10733)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10768..10787
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacr kkrchygckr
      301 ymagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
      361 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
      421 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      481 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      541 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      601 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      661 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      721 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      781 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      841 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      901 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      961 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     1021 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1081 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
     1141 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1201 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1261 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1321 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1381 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1441 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1501 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1561 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1621 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1681 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1741 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1801 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1861 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     1921 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc
     1981 ctttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     2041 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     2101 ggtttattac agggacagca gagatccagt ttatcgatga ggccctttcg tcttcactcg
     2161 aggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     2221 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     2281 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     2341 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg
     2401 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc
     2461 cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
     2521 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
     2581 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     2641 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     2701 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
     2761 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     2821 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     2881 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     2941 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
     3001 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
     3061 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     3121 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     3181 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     3241 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     3301 tcaaagtttt tttcttccat ttcaggtgtc gtgaggatcc atggcacacc atcaccacca
     3361 tcacggatct ggttctagcc tgggccccgt agagctgctg ctgcggggct cctcgcgccg
     3421 cctcgacctg ctgcaccagc agctgcagga gctgcacgcc cacggttctg gttccctgct
     3481 agagcagctg ggcctggccg gggcagacct ggcggccccc ggggtacagc agcagctgga
     3541 gctggagcgg gagcggctgc ggcgggaaat ccgcaaggag ctgaagctga aggagggtgc
     3601 tgagaaccag cggcgggcca ccactgacct gggccgcggt accggttctg acggcggcgt
     3661 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     3721 cgacaaccac tacctgagct accagtccgc actgagcaaa gaccccaacg agaagcgcga
     3781 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     3841 gtacaagggt tctggaagtg gttctgtgag caagggcgag gagctgttca ccggggtggt
     3901 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     3961 gggcgagggc gatgccacct acggcaagct gaccctgaag ttgatctgca ccaccggcaa
     4021 gctgcccgtg ccctggccca ccctcgtgac caccctcggc tacggcctgc agtgcttcgc
     4081 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     4141 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     4201 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     4261 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     4321 caccgccgac aagcagaaga acggcatcaa ggcaaacttc aagatccgcc acaacatcga
     4381 gggatcagaa gcacaaaagc agaaggaaca gagacaggca gcagaggagc tggcaaacgg
     4441 atcggaggct cagaaacaaa aagagcaaag gcaagctgct gaagaactag ctaatggttc
     4501 tgaggcgcaa aagcagaaag aacaacgtca ggccgccgaa gagttagcga acggatccgg
     4561 gagtgtttcc aaaggggaag aattgtttac cggcgtggtc cccattctcg tggaacttga
     4621 tggcgatgtg aatggacaca aattctccgt aagcggagag ggtgagggtg atgctactta
     4681 cgggaagttg actcttaagt ttatttgcac gactgggaaa ctccccgtcc cttggcctac
     4741 tctggtgact acactgagtt ggggagttca atgctttgcc cgatatcctg accatatgaa
     4801 acagcatgac ttttttaaat cagctatgcc tgaggggtat gttcaggaac gcactatctt
     4861 tttcaaagac gatgggaatt ataaaaccag ggctgaagtg aaatttgagg gtgacacact
     4921 ggtcaatcga atcgaactga aaggaattga ctttaaagaa gatgggaaca ttcttggaca
     4981 caaactggaa tacaatgcga tccacgggaa cgtgtacatc actgccgata aacagaaaaa
     5041 tgggatcaaa gccaatttcg gacttaattg taacattgaa gatggcagtg tgcaacttgc
     5101 cgatcactat cagcaaaata ccccgatcgg agacggacct gtgctcctgc cagataacca
     5161 ttatctgagt acgcagtctg cactcagcaa ggaccctaat gagaagagag accatatggt
     5221 gctcctggaa tttgtcaccg ctgccggcgc tagcgcggcc atccgcaaga agctggtggt
     5281 tgtgggcgac ggcgcgtgtg gcaagacgtg cctgctgatc gtgttcagta aggacgagtt
     5341 ccccgaggtg tacgtgccca ccgtcttcga gaactatgtg gccgacattg aggtggacgg
     5401 caagcaggtg gagctggcgc tgtgggacac ggcgggccag gaggactacg accgcctgcg
     5461 gccgctctcc tacccggaca ccgacgtcat tctcatgtgc ttctcggtgg acagcccgga
     5521 ctcgctggag aacatccccg agaagtgggt ccccgaggtg aagcacttct gtcccaatgt
     5581 gcccatcatc ctggtggcca acaaaaaaga cctgcgcagc gacgagcatg tccgcacaga
     5641 gctggcccgc atgaagcagg aacccgtgcg cacggatgac ggccgcgcca tggccgtgcg
     5701 catccaagcc tacgactacc tcgagtgctc tgccaagacc aaggaaggcg tgcgcgaggt
     5761 cttcgagacg gccacgcgcg ccgcgctgca gaagcgctac ggctcccaga acggctgcat
     5821 caactgctgc aaggtgctat gagcggccgc tctagagtcg acgggccgcg gtaacaattg
     5881 ttaactaact taagctagca acggtttccc tctagcggga tcaattccgc cccccccccc
     5941 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     6001 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     6061 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     6121 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     6181 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     6241 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     6301 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     6361 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     6421 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     6481 aacacgataa taccatggtc atgaaaacat ttaacatttc tcaacaagat ctagaattag
     6541 tagaagtagc gacagagaag attacaatgc tttatgagga taataaacat catgtgggag
     6601 cggcaattcg tacgaaaaca ggagaaatca tttcggcagt acatattgaa gcgtatatag
     6661 gacgagtaac tgtttgtgca gaagccattg cgattggtag tgcagtttcg aatggacaaa
     6721 aggattttga cacgattgta gctgttagac acccttattc tgacgaagta gatagaagta
     6781 ttcgagtggt aagtccttgt ggtatgtgta gggagttgat ttcagactat gcaccagatt
     6841 gttttgtgtt aatagaaatg aatggcaagt tagtcaaaac tacgattgaa gaactcattc
     6901 cactcaaata tacccgaaat taacccgggc ggggcgcgtc tggaacaatc aacctctgga
     6961 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7021 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7081 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7141 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7201 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7261 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7321 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     7381 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7441 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7501 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac
     7561 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     7621 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     7681 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga
     7741 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     7801 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     7861 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     7921 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     7981 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     8041 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
     8101 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
     8161 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
     8221 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8281 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8341 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     8401 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta
     8461 gcgtttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     8521 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8581 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8641 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8701 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8761 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8821 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8881 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8941 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9001 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9061 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9121 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9181 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9241 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9301 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     9361 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9421 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9481 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9541 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9601 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9661 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9721 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9781 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9841 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9901 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9961 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    10021 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    10081 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    10141 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10201 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10261 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10321 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10381 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10441 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10501 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10561 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10621 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact
    10681 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    10741 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    10801 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
    10861 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
    10921 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagtt
//
