LOCUS       sequence-439113-        8411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible 
            with piggybac transposase for genome integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Talaia G, Bentley-DeSousa A, Ferguson SM
  TITLE     Lysosomal TBK1 responds to amino acid availability to relieve 
            Rab7-dependent mTORC1 inhibition.
  JOURNAL   EMBO J. 2024 Sep;43(18):3948-3967. doi: 10.1038/s44318-024-00180-8. 
            Epub 2024 Aug 5.
  PUBMED    39103493
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00180-8"; journalName: "EMBO J"; date: 
            "2024-09"; volume: "43"; issue: "18"; pages: "3948-3967"
COMMENT     Sequence Label: pPB-EF1A-Puro-mCherry-RAB7
FEATURES             Location/Qualifiers
     source          1..8411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(634..654)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     repeat_region   931..965
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(1114..1132)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1153..1169)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1153..1169)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1166..1188)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1177..1193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1201..1207,1208..1225,1226..1231))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1246..1267
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1384..1401)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1555..2143)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1635..1654)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2314..3105,3106..3174))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2937..2956
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3175..3279)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3305..3760)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3442..3463)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3654..3673
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3887..3909
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3901..3918
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3902..3918
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3925..3944
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3925..3943
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   4010..4072
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    4422..4646
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4635..4652
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(4690..5289)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4773..4793)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5270..5289
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(5322..5525)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5351..5371)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5526..5905
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     6242..6261
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    6260..6315
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     6361..6380
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     protein_bind    6458..6482
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7128..7835)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7403..7422)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7672..7690
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      7832..7841
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(7842..7866)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(join(7866..8411,1..633))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(7875..8411,1..402)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(7898..7918)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 gcgctgggac gcacgggccc cgtcgccgcc cgcggcccca aaaaccgaaa taccagtgtg
       61 cagatcttgg cccgcattta caagactatc ttgccagaaa aaaagcgtcg cagcaggtca
      121 tcaaaaattt taaatggcta gagacttatc gaaagcagcg agacaggcgc gaaggtgcca
      181 ccagattcgc acgcggcggc cccagcgccc aggccaggcc tcaactcaag cacgaggcga
      241 aggggctcct taagcgcaag gcctcgaact ctcccaccca cttccaaccc gaagctcggg
      301 atcaagaatc acgtactgca gccaggtgga agtaattcaa ggcacgcaag ggccataacc
      361 cgtaaagagg ccaggcccgc gggaaccaca cacggcactt acctgtgttc tggcggcaaa
      421 cccgttgcga aaaagaacgt tcacggcgac tactgcactt atatacggtt ctcccccacc
      481 ctcgggaaaa aggcggagcc agtacacgac atcactttcc cagtttaccc cgcgccacct
      541 tctctaggca ccggttcaat tgccgacccc tccccccaac ttctcgggga ctgtgggcga
      601 tgtgcgctct gcccactgac gggcaccgga gcccaacttt tctatacaaa gttgatgata
      661 tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa
      721 tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt
      781 gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct
      841 cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat
      901 ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc
      961 tagggttaaa aaagatttgc gctttactcg acctaaactt taaacacgtc atagaatctt
     1021 cgtttgacaa aaaccacatt gtggccaagc tgtgtgacgc gacgcgcgct aaagaatggc
     1081 aaaccaagtc gcgcgaggta ccagcttttg ttccctttag tgagggttaa tttcgagctt
     1141 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     1201 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     1261 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     1321 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     1381 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1441 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1501 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1561 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1621 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1681 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1741 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1801 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1861 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1921 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1981 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2041 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2101 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2161 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2221 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2281 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2341 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2401 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     2461 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2521 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2581 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2641 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     2701 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2761 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2821 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2881 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2941 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3001 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3061 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3121 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3181 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3241 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3301 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     3361 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     3421 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     3481 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     3541 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     3601 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     3661 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     3721 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     3781 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     3841 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     3901 tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt ggagctcggt
     3961 attcacgaca gcaggctgaa taataaaaaa attagaaact attatttaac cctagaaaga
     4021 taatcatatt gtgacgtacg ttaaagataa tcatgcgtaa aattgacgca tgtgttttat
     4081 cggtctgtat atcgaggttt atttattaat ttgaatagat attaagtttt attatattta
     4141 cacttacata ctaataataa attcaacaaa caatttattt atgtttattt atttattaaa
     4201 aaaaaacaaa aactcaaaat ttcttctata aagtaacaaa acttttaaac attctctctt
     4261 ttacaaaaat aaacttattt tgtactttaa aaacagtcat gttgtattat aaaataagta
     4321 attagcttaa cctatacata atagaaacaa attatactta ttagtcagtc agaaacaact
     4381 ttggcacata tcaatattat gctctcgtta attaactcga gccatagagc ccaccgcatc
     4441 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
     4501 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
     4561 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
     4621 agatggctgg caactagaag gcacagtcga ggctgatcag cgggtttaaa cgggccctct
     4681 agactcgagt caggcaccgg gcttgcgggt catgcaccag gtgcgcggtc cttcgggcac
     4741 ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag aaggggaggt tgcggggcgc
     4801 ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc gcctccactc cggggagcac
     4861 gacggcgctg cccagaccct tgccctggtg gtcgggcgag acgccgacgg tggccaggaa
     4921 ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct tccatctgtt gctgcgcggc
     4981 cagccgggaa ccgctcaact cggccatgcg cgggccgatc tcggcgaaca ccgcccccgc
     5041 ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg ccgtcgtccg cgacccacac
     5101 cttgccgatg tcgagcccga cgcgcgtgag gaagagttct tgcagctcgg tgacccgctc
     5161 gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg tagtcggcga acgcggcggc
     5221 gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg aggcgcaccg tgggcttgta
     5281 ctcggtcatg gtggcgcagt gggttctcta gttagccaga gagctctgct tatatagacc
     5341 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
     5401 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
     5461 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
     5521 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     5581 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     5641 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     5701 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     5761 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     5821 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
     5881 taataactag tcaataatca atgtcaacgc gaagcttggg ctgcaggtcg agggatctcc
     5941 ataagagaag agggacagct atgactggga gtagtcagga gaggaggaaa aatctggcta
     6001 gtaaaacatg taaggaaaat tttagggatg ttaaagaaaa aaataacaca aaacaaaata
     6061 taaaaaaaat ctaacctcaa gtcaaggctt ttctatggaa taaggaatgg acagcagggg
     6121 gctgtttcat atactgatga cctctttata gccaaccttt gttcatggca gccagcatat
     6181 gggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa tgatttgccc
     6241 tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt gcaatgaaaa
     6301 taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg tccccataat
     6361 ttttggcaga gggaaaaaga tctcagtggt atttgtgagc cagggcattg gccacaccag
     6421 ccaccacctt ctgataggca gcctgcacct gaggatcacc actttgtaca agaaagctgg
     6481 gttcagcaac tgcagctttc tgccgaggcc ttggcccggt cattcttgtc cagtttgata
     6541 ggttcaggaa attcgttgta cagctccacc tccgtttcct gcttaagtgc attccgtgca
     6601 atcgtctgga acgcctgctc cacgttgatg gcctccttgg cactggtctc aaagtaggga
     6661 atgttgtttt tgctgtagca ccaggcctgt gcccgctttg tggccacttg tctgttttcg
     6721 aggtcaatct tgtttcccaa cacaacaaat gggaagtttt caggatctcg gggactggcc
     6781 tggatgagaa actcatctct ccagctatct agggttttga atgtgttggg ggcagtcaca
     6841 tcaaatacca gaacgcagca gtctgcacct ctgtagaagg ccacaccgag agactggaac
     6901 cgttcctgtc ctgctgtgtc ccatatctgc attgtgacta gcctgtcatc caccatcacc
     6961 tccttggtca gaaagtcagc tcctattgtg gctttgtact gattgctgaa tttcttattc
     7021 acatactggt tcatgagtga tgtcttcccg actccagaat ctcccaggat gataaccttc
     7081 agcaacactt tcttcctaga ggtcatagct cgagatctga gtccggactt gtacagctcg
     7141 tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt actgttccac gatggtgtag
     7201 tcctcgttgt gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc
     7261 acgggcttct tggccttgta ggtggtcttg acctcagcgt cgtagtggcc gccgtccttc
     7321 agcttcagcc tctgcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg
     7381 gaggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg
     7441 gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc
     7501 acggtcacca cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg
     7561 aaggacagct tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg
     7621 tacatgaact gaggggacag gatgtcccag gcgaagggca gggggccacc cttggtcacc
     7681 ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg
     7741 aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa ctccttgatg
     7801 atggccatgt tatcctcctc gcccttgctc accatggtgg cagcctgctt ttttgtacaa
     7861 acttgtcacg acacctgaaa tggaagaaaa aaactttgaa ccactgtctg aggcttgaga
     7921 atgaaccaag atccaaactc aaaaagggca aattccaagg agaattacat caagtgccaa
     7981 gctggcctaa cttcagtctc cacccactca gtgtggggaa actccatcgc ataaaacccc
     8041 tccccccaac ctaaagacga cgtactccaa aagctcgaga actaatcgag gtgcctggac
     8101 ggcgcccggt actccgtgga gtcacatgaa gcgacggctg aggacggaaa ggcccttttc
     8161 ctttgtgtgg gtgactcacc cgcccgctct cccgagcgcc gcgtcctcca ttttgagctc
     8221 cctgcagcag ggccgggaag cggccatctt tccgctcacg caactggtgc cgaccgggcc
     8281 agccttgccg cccagggcgg ggcgatacac ggcggcgcga gaccaggcac cagagcaggc
     8341 cggccagctt gagactaccc ccgtccgatt ctcggtggcc gcgctcgcag gccccgcctc
     8401 gccgaacatg t
//
