LOCUS       sequence-439118-        8411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian 
            cells. Compatible with piggybac transposase for genome integration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Talaia G, Bentley-DeSousa A, Ferguson SM
  TITLE     Lysosomal TBK1 responds to amino acid availability to relieve 
            Rab7-dependent mTORC1 inhibition.
  JOURNAL   EMBO J. 2024 Sep;43(18):3948-3967. doi: 10.1038/s44318-024-00180-8. 
            Epub 2024 Aug 5.
  PUBMED    39103493
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-024-00180-8"; journalName: "EMBO J"; date: 
            "2024-09"; volume: "43"; issue: "18"; pages: "3948-3967"
COMMENT     Sequence Label: pPB-EF1A-Puro-mCherry-RAB7-S72A
FEATURES             Location/Qualifiers
     source          1..8411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             44..751
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(189..207)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     457..476
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(1397..1421)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1499..1518)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1564..1619
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1618..1637)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        1974..2353
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2354..2557
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2508..2528
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2590..3189
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2590..2609)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3086..3106
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(3227..3244)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3233..3457
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3807..3869
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3935..3954)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3936..3954)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3961..3978)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3961..3977)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3970..3992)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4119..4574
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4206..4225)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4416..4437
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4600..4704
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4705..4773,4774..5565)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4923..4942)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5736..6324
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6225..6244
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6478..6495
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6612..6633
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6648..6653,6654..6671,6672..6678)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6686..6702
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6691..6713
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6710..6726
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6710..6726
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        6747..6765
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   6914..6948
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    7225..7245
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        join(7246..8411,1..13)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(7477..8411,1..4)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8372..8392
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 tcaggtgtcg tgacaagttt gtacaaaaaa gcaggctgcc accatggtga gcaagggcga
       61 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      121 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      181 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      241 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
      301 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
      361 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
      421 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
      481 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
      541 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
      601 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
      661 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
      721 ctccaccggc ggcatggacg agctgtacaa gtccggactc agatctcgag ctatgacctc
      781 taggaagaaa gtgttgctga aggttatcat cctgggagat tctggagtcg ggaagacatc
      841 actcatgaac cagtatgtga ataagaaatt cagcaatcag tacaaagcca caataggagc
      901 tgactttctg accaaggagg tgatggtgga tgacaggcta gtcacaatgc agatatggga
      961 cacagcagga caggaacggt tccaggctct cggtgtggcc ttctacagag gtgcagactg
     1021 ctgcgttctg gtatttgatg tgactgcccc caacacattc aaaaccctag atagctggag
     1081 agatgagttt ctcatccagg ccagtccccg agatcctgaa aacttcccat ttgttgtgtt
     1141 gggaaacaag attgacctcg aaaacagaca agtggccaca aagcgggcac aggcctggtg
     1201 ctacagcaaa aacaacattc cctactttga gaccagtgcc aaggaggcca tcaacgtgga
     1261 gcaggcgttc cagacgattg cacggaatgc acttaagcag gaaacggagg tggagctgta
     1321 caacgaattt cctgaaccta tcaaactgga caagaatgac cgggccaagg cctcggcaga
     1381 aagctgcagt tgctgaaccc agctttcttg tacaaagtgg tgatcctcag gtgcaggctg
     1441 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
     1501 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     1561 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     1621 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     1681 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     1741 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     1801 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     1861 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     1921 ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttcgc gttgacattg
     1981 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     2041 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2101 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2161 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2221 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2281 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2341 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2401 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2461 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2521 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
     2581 tgcgccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     2641 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     2701 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     2761 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     2821 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     2881 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     2941 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     3001 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     3061 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     3121 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     3181 ggtgcctgac tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
     3241 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     3301 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     3361 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     3421 aatagcaggc atgctgggga tgcggtgggc tctatggctc gagttaatta acgagagcat
     3481 aatattgata tgtgccaaag ttgtttctga ctgactaata agtataattt gtttctatta
     3541 tgtataggtt aagctaatta cttattttat aatacaacat gactgttttt aaagtacaaa
     3601 ataagtttat ttttgtaaaa gagagaatgt ttaaaagttt tgttacttta tagaagaaat
     3661 tttgagtttt tgtttttttt taataaataa ataaacataa ataaattgtt tgttgaattt
     3721 attattagta tgtaagtgta aatataataa aacttaatat ctattcaaat taataaataa
     3781 acctcgatat acagaccgat aaaacacatg cgtcaatttt acgcatgatt atctttaacg
     3841 tacgtcacaa tatgattatc tttctagggt taaataatag tttctaattt ttttattatt
     3901 cagcctgctg tcgtgaatac cgagctccaa ttcgccctat agtgagtcgt attacaattc
     3961 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     4021 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     4081 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     4141 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4201 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4261 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4321 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4381 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4441 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4501 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4561 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4621 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4681 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4741 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4801 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4861 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4921 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4981 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5041 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5101 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5161 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5221 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5281 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5341 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5401 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5461 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5521 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5581 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5641 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5701 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5761 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5821 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5881 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5941 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6001 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6061 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6121 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6181 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6241 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6301 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6361 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6421 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6481 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6541 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     6601 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     6661 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     6721 tatgaccatg attacgccaa gctcgaaatt aaccctcact aaagggaaca aaagctggta
     6781 cctcgcgcga cttggtttgc cattctttag cgcgcgtcgc gtcacacagc ttggccacaa
     6841 tgtggttttt gtcaaacgaa gattctatga cgtgtttaaa gtttaggtcg agtaaagcgc
     6901 aaatcttttt taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
     6961 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
     7021 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
     7081 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
     7141 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
     7201 atattttctt gttatagata tcatcaactt tgtatagaaa agttgggctc cggtgcccgt
     7261 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     7321 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     7381 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     7441 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     7501 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     7561 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     7621 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     7681 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     7741 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     7801 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     7861 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     7921 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggtct cgcgccgccg
     7981 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     8041 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     8101 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     8161 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     8221 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     8281 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     8341 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     8401 tttcttccat t
//
