LOCUS       sequence-43912-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Subach OM, Entenberg D, Condeelis JS, Verkhusha VV
  TITLE     A FRET-facilitated photoswitching using an orange fluorescent 
            protein with the fast photoconversion kinetics.
  JOURNAL   J Am Chem Soc. 2012 Aug 17.
  PUBMED    22900938
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2012 Aug 17."
COMMENT     Sequence Label: pPSmOrange2-Tubulin
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="enhanced orange to far-red photoswitchable 
                     monomeric fluorescent protein (Subach et al., 2012)"
                     /label=PSmOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGTVNGHEFEIEGEGEGHPYEG
                     FQTAKLKVTKGGPLPFAWDILSPLITYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIRMRLKLKDGGHYTSEVKTTYKAKKSVLLPGAYIVGIKLDITSHNEDYTIVEQYERS
                     EARHSTGGMDELYK"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agtgaaccgt cagatccgct agcggtcgcc accatggtga gcaagggcga ggagaataat
       61 atggccatca ttaaggagtt catgcgcttc aaggtgcaca tggagggcac tgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccacccct acgagggctt tcagaccgct
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctctc
      241 atcacctacg gctccaaggc gtacgtgaag caccccgccg acatccccga ctacttcaag
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga actacgagga cggcggcgtg
      361 gtgaccgtga cccaggactc ctctctgcag gacggcgagt tcatctacaa ggtgaagatg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caggatgagg
      541 ctgaagctga aggatggcgg ccactacacc tccgaggtca agactaccta caaggccaag
      601 aagtccgtgc tgctgcccgg cgcctacatc gtcggcatca agttggacat tacctcacac
      661 aacgaggact acaccatcgt ggaacagtat gaacgcagcg aggcccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctcgag tgcgtgagtg catctccatc
      781 cacgttggcc aggctggtgt ccagattggc aatgcctgct gggagctcta ctgcctggaa
      841 cacggcatcc agcccgatgg ccagatgcca agtgacaaga cc
//
