LOCUS       sequence-439140-        9376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express an early endosome ""anchor"" for rapamycin-induced 
            heterodimerisation in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9376)
  AUTHORS   Downie L, Ferrandiz N, Courthold E, Jones M, Royle SJ
  TITLE     Nondisruptive inducible labeling of ER-membrane contact sites using 
            the Lamin B receptor.
  JOURNAL   PLoS Biol. 2025 Jul 10;23(7):e3003249. doi: 
            10.1371/journal.pbio.3003249. eCollection 2025 Jul.
  PUBMED    40638559
REFERENCE   2  (bases 1 to 9376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2025-07-10"; volume: "23(7):e3003249. doi"; pages: " 
            10.1371/journal.pbio.3003249. eCollection 2025 Jul"
COMMENT     Sequence Label: FRB-mCherry-EEA1
FEATURES             Location/Qualifiers
     source          1..9376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             607..885
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             619..888
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     regulatory      904..913
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             910..1617
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1055..1073)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1323..1342
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1639..5874
                     /codon_start=1
                     /product="human early endosome antigen 1"
                     /label=EEA1
                     /translation="MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQ
                     CMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKW
                     YSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQ
                     KAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIED
                     VAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQ
                     EVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLH
                     QKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQ
                     QQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADL
                     QLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQ
                     IGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQ
                     VTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKE
                     KVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVT
                     AKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDL
                     QQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKQIL
                     KQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKN
                     SKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLK
                     LELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQK
                     TELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQD
                     LKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQD
                     IQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQ
                     LLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEK
                     EVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEAT
                     VQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQ
                     ALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVC
                     DACFNDLQG"
     polyA_signal    6164..6285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6201..6220)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6255..6274
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6292..6747)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6429..6450)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6641..6660
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6774..6878
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6880..7237
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6880..7071
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6903..6923)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7088..7223
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7272..8066
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7272..8066
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7326..7345)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7936..7955
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8254..8273)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8298..8345
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8674..9262
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9163..9182
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcggcc
      601 accatgatcc tctggcatga gatgtggcat gaaggcctgg aagaggcatc tcgtttgtac
      661 tttggggaaa ggaacgtgaa aggcatgttt gaggtgctgg agcccttgca tgctatgatg
      721 gaacggggcc cccagactct gaaggaaaca tcctttaatc aggcctatgg tcgagattta
      781 atggaggccc aagagtggtg caggaagtac atgaaatcag ggaatgtcaa ggacctcacc
      841 caagcctggg acctctatta tcatgtgttc cgacgaatct caaagcaggc cggcccaccg
      901 gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
      961 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     1021 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     1081 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     1141 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     1201 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     1261 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     1321 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     1381 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     1441 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     1501 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     1561 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtcc
     1621 ggactcagat ctcgagaaat gttaaggagg attttacaga ggactcctgg gagagttggc
     1681 tctcaaggtt ctgatttaga ttcatcagca actcctataa acacagtgga cgtcaataat
     1741 gaaagctctt cagaaggttt catatgtccc cagtgtatga aatctcttgg atctgctgat
     1801 gaacttttca aacattatga agctgttcat gatgctggta atgactcagg tcatggagga
     1861 gagtctaatc ttgctttgaa gcgagatgat gtaacactgc tcagacaaga ggtccaagac
     1921 ctacaggctt cacttaagga agaaaaatgg tactcggaag aattaaagaa ggaattagaa
     1981 aaatatcaag ggctgcagca gcaagaggcc aaacctgatg ggttggtgac tgattcatca
     2041 gcagaactac agtctttgga acagcaatta gaagaagccc aaacagaaaa ttttaatatt
     2101 aagcaaatga aagacttatt tgaacagaaa gcagcccaac ttgctactga aattgcagat
     2161 ataaagtcaa agtatgatga agaaaggagt cttcgagaag ctgctgaaca aaaagtgaca
     2221 cgtctgacag aagaattaaa caaagaggca actgtaattc aagatctgaa gacggaactg
     2281 cttcagagac ctggtataga agatgttgcc gtgctaaaga aagaactggt ccaagttcaa
     2341 acactaatgg ataacatgac cttggaacgt gagcgagaat ctgaaaaact caaagatgaa
     2401 tgcaaaaaat tgcagtcaca atatgctagc tcagaggcca caataagcca gctaaggagt
     2461 gaacttgcca aaggccccca ggaagttgct gtatatgtac aggaactaca aaaactgaaa
     2521 agttcagtta atgaattaac acaaaaaaat cagaccttga cagaaaactt gctgaaaaaa
     2581 gaacaagact atactaagtt agaggagaaa cataatgaag aatctgtgag taaaaagaat
     2641 attcaggcaa cccttcatca aaaagaccta gattgtcaac agcttcagtc aagattgtct
     2701 gcatctgaaa cctcactgca tagaatacat gtagaactaa gtgaaaaagg agaagctact
     2761 caaaagctca aagaagaatt atctgaggta gagaccaagt accagcatct aaaggcggag
     2821 tttaagcagc tacaacaaca gagagaagaa aaggagcagc atgggttaca actccaaagt
     2881 gaaattaatc aattacatag caaacttcta gagacagagc gccaactagg ggaagctcat
     2941 ggtaggctga aggaacagag acagctttca agtgaaaagt tgatggataa agaacaacaa
     3001 gtggctgatt tacaactcaa actttctcgg ttagaagagc agttgaagga aaaagttaca
     3061 aattctacag aattgcagca tcaattagat aaaacaaagc aacagcatca agaacaacag
     3121 gctcttcagc aaagcaccac ggcaaaactt cgagaagctc agaatgattt ggaacaagtt
     3181 ctacgtcaaa ttggcgacaa ggaccaaaag atccagaacc ttgaagcttt attacagaag
     3241 agtaaagaaa atatttcatt actagaaaaa gaaagagaag atctttatgc aaaaattcag
     3301 gctggtgaag gagagactgc tgttcttaac cagttacaag aaaaaaacca tacactacag
     3361 gagcaagtaa ctcaactaac agagaagctg aagaatcagt cagaaagtca taaacaagcc
     3421 caggagaatt tgcatgacca ggtacaagag cagaaggcac atcttagagc tgcacaagac
     3481 cgtgtccttt ccctagaaac tagtgtcaat gaattaaata gtcaattaaa tgaaagcaag
     3541 gagaaggtct cccagcttga catacagatt aaagccaaaa ccgaactatt actatcagca
     3601 gaagcagcaa aaactgctca aagagctgat cttcagaatc atttggacac agctcaaaat
     3661 gcattacaag ataaacagca ggagttaaat aagattacta ctcagttgga tcaggtcact
     3721 gcaaagttac aagacaagca agaacattgc agtcagctgg aaagtcatct taaagaatat
     3781 aaagagaaat acctctcttt agaacagaaa accgaagagc tagaaggtca aattaagaaa
     3841 ctagaagctg atagtcttga agttaaagca agcaaggagc aggctttgca agatctacaa
     3901 cagcaaagac agctgaacac agatttagag ctcagagcca cagaattgag taaacaactt
     3961 gaaatggaga aggaaatagt atccagtaca agattggatc tacagaaaaa atctgaagcc
     4021 cttgaaagta tcaagcaaaa gcttaccaag caagaggaag aaaaacaaat cctgaaacaa
     4081 gattttgaaa ctttaagtca agaaacaaag attcagcatg aggaattgaa taacagaatt
     4141 caaacaacag taacagaact acaaaaagtg aaaatggaga aagaagcttt aatgacagag
     4201 ctttctacag taaaggacaa actatcaaaa gtttctgatt ctttgaaaaa ctctaaaagt
     4261 gaatttgaaa aggagaatca gaaaggaaaa gccgctatat tagacttgga aaaaacttgc
     4321 aaagaattaa agcatcaact tcaagtgcag atggaaaaca cacttaagga acagaaggaa
     4381 ctgaaaaagt cacttgaaaa agagaaggag gcttctcatc agttgaaatt ggaactcaat
     4441 tcaatgcagg aacaacttat acaggcccag aatactttaa aacaaaatga aaaagaagag
     4501 caacaacttc aggggaacat aaatgagcta aagcaatcaa gtgaacagaa gaaaaaacaa
     4561 attgaagcac tccaaggaga gcttaaaatt gctgttttac agaagacaga gcttgagaat
     4621 aaactacagc agcagttaac acaggcagcc caggaacttg cagcagagaa agagaaaata
     4681 tcagtattac aaaacaacta tgaaaaaagt caggaaactt tcaaacagct tcaatctgat
     4741 ttctatggga gggaatctga acttctagcc accaggcaag atcttaagtc tgtagaagag
     4801 aagctttctc tagcacagga ggacttgatt tcaaacagaa atcaaattgg aaatcaaaat
     4861 aaattgattc aagaactgaa gactgccaag gctacattgg agcaggattc agcaaagaaa
     4921 gaacagcaat tgcaggagcg atgtaaagca ctacaagaca ttcagaaaga aaagtcactg
     4981 aaagaaaaag aactggtaaa tgagaagtct aaattggcag agatagaaga aattaaatgt
     5041 agacaagaaa aagaaatcac taaactaaac gaagaactca agtcccacaa actagaaagc
     5101 ataaaggaga taacaaatct taaagatgct aaacagcttc taattcagca gaaattagaa
     5161 cttcaaggaa aagcggactc cctgaaggca gctgttgaac aggagaagag aaatcagcag
     5221 atactaaaag accaggtgaa aaaggaagaa gaggagctga agaaagaatt tattgagaaa
     5281 gaagctaagt tgcattccga aataaaagaa aaggaagtag gaatgaagaa gcatgaagaa
     5341 aatgaggcta aacttaccat gcagattaca gcattaaatg aaaacttagg cactgtgaag
     5401 aaggagtggc aatctagtca acggagagtt agtgagcttg agaaacaaac ggatgactta
     5461 cggggtgaaa ttgcagtatt agaagcaacg gttcagaata atcaagatga aaggagagca
     5521 ctactggaaa gatgtcttaa aggagaaggt gaaatagaaa agcttcaaac caaagtatta
     5581 gaattgcaaa gaaagctgga taatacaact gcagcagtgc aggagctggg cagagaaaac
     5641 caatcacttc agatcaaaca tacacaagcg ttgaatagaa agtgggccga agacaatgaa
     5701 gtacaaaact gtatggcctg tgggaaaggc ttttcagtaa cagtgagacg gcatcactgc
     5761 cgacagtgtg gaaatatctt ctgtgctgaa tgttcagcca aaaatgcctt aactccttcc
     5821 tccaagaagc ctgttcgtgt ctgtgatgca tgtttcaatg acttgcaagg ataatgggtt
     5881 atcacaactt cagagtaata ttacactaac attagatttt taataaatgt acttaataga
     5941 ggtcttggac tactatttgg tttggacact ggttgcaata ctagaccaaa taggaattag
     6001 tatattttgg aatgttatgg atatcgcggc cgctggatcc accggatcta gataactgat
     6061 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     6121 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     6181 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6241 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa
     6301 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     6361 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     6421 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     6481 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     6541 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     6601 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     6661 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     6721 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     6781 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6841 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     6901 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6961 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     7021 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     7081 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7141 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7201 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     7261 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     7321 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     7381 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     7441 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     7501 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     7561 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     7621 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     7681 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     7741 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     7801 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     7861 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     7921 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     7981 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     8041 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     8101 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     8161 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     8221 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     8281 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     8341 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     8401 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     8461 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
     8521 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8581 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8641 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8701 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8761 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8821 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8881 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     8941 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     9001 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     9061 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     9121 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     9181 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     9241 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     9301 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     9361 tattaccgcc atgcat
//
