LOCUS       sequence-439155-        9323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of FES (pLxIS) in mammalian cells by retroviral 
            transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Landau LM, Chaudhary N, Tien YC, Rogozinska M, Joshi S, Yao C, 
            Crowley J, Hullahalli K, Campbell IW, Waldor MK, Haigis M, Kagan JC
  TITLE     pLxIS-containing domains are biochemically flexible regulators of 
            interferons and metabolism.
  JOURNAL   Mol Cell. 2024 Jul 11;84(13):2436-2454.e10. doi: 
            10.1016/j.molcel.2024.05.030. Epub 2024 Jun 25.
  PUBMED    38925114
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.05.030"; journalName: "Mol Cell"; date: 
            "2024-07-11- 11"; volume: "84"; issue: "13"; pages: "2436-2454.e10"
COMMENT     Sequence Label: pMSCV-FKBP-FES
FEATURES             Location/Qualifiers
     source          1..9323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..39
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2946..3512
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2962..3514
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3106..3123)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3333..3352
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3526..3528,3529..3531,3532..4245)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3571..3592)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3832..3851)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4179..4200
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             4307..4821
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    4983..4999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5007..5013,5014..5031,5032..5037))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5052..5073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5190..5207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5361..5949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5441..5460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6120..6911,6912..6980))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6743..6762
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6981..7085)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7153..7171
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7209..7231)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7331..7350
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7544..7566
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7694..7713
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             7913..8429
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    8493..8834
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             8901..9317
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     9247..9269
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     9285..9301
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 agatctgcca ccatggacta caaagacgat gacgacaagg gcgtccaagt ggagacaatc
       61 tctcctgggg atggaaggac attccccaag cggggtcaga cctgcgttgt tcattatacc
      121 ggtatgttgg aagacggtaa aaaagtcgat agctcacggg atcgcaataa gcctttcaag
      181 ttcatgcttg ggaaacagga agtaatacgc ggctgggaag agggagtagc tcaaatgtcc
      241 gtcggtcaac gcgccaaatt gacaatttct cctgactacg cttatggtgc aaccggacac
      301 cccggcataa ttcccccaca cgcaacactt gtatttgatg tagaattgct caagctggaa
      361 acaagaggag tccaggttga aaccatatct cctggggacg gaagaacctt ccctaagcgg
      421 ggacaaactt gcgttgtcca ttacacaggc atgcttgaag atggtaagaa agtcgactca
      481 tcacgagaca gaaataagcc attcaaattt atgctcggta aacaggaagt cataagaggc
      541 tgggaagaag gagtggccca aatgtctgta ggacaacgag ccaaactcac aatatctccc
      601 gattatgctt atggtgccac tggccacccc ggcattatac cacctcatgc cactcttgtt
      661 tttgacgtag agctgttgaa gttggagacc cggggcgtgc aggttgagac tataagtcct
      721 ggagatggaa ggacttttcc taaaagaggt cagacatgcg tagtacacta tacagggatg
      781 ttggaggacg gcaaaaaagt cgacagctcc cgagaccgga ataagccttt taagttcatg
      841 ttggggaaac aggaagtaat ccgcgggtgg gaagagggag tcgctcagat gtctgtggga
      901 cagcgcgcta agctgaccat ttcccccgat tatgcctacg gcgctaccgg tcaccccggg
      961 attattccac ctcacgctac tttggtgttc gatgtcgaac ttctgaaact ggagactcgg
     1021 ggagtacagg ttgaaacaat cagcccagga gacggaagaa cctttccaaa aaggggtcaa
     1081 acatgtgtgg tacactacac aggaatgttg gaagacggaa aaaaggtgga cagctctcga
     1141 gaccgcaata aacctttcaa gtttatgctg ggcaaacagg aggtgatacg cggatgggag
     1201 gaaggcgtag cccagatgtc cgtgggtcag cgggcaaaac ttacaatttc acccgattac
     1261 gcatatggcg caacaggaca tccaggtatt ataccaccac atgccaccct tgttttcgac
     1321 gtagagcttt tgaaactcga aggggctgga gcaggcgctg caggcatctt gaaggacata
     1381 ctgcaagagt atcttgaaat ctctagcctc gtgcaggatg atgttgcctc catacatcgc
     1441 gaactggctg ctgctgctgc tcggatacaa ccagaatttg agtatttggg ttttttgcgc
     1501 cagtatggta gtactcccga tgtccctcca tgtgttacat ttgacgaatc cttgctcgag
     1561 gacggggaac agttggagcc cggggagctt cagctcaacg aattgaccct cgaatctgtg
     1621 caacacacac ttactagcgt cactgatgaa ctcgccgttg ctacaaagga agtcctgagt
     1681 agacaggaga tggtttctca gttgcagagg gaattgcaga gcgaagaaca gaatacacat
     1741 ccacgggaaa gggtccagct tcttagcaag aggcaaatgc ttcaggaggc catccaggga
     1801 ttgcaaatag ctctgtgttc acaagataaa ctccaagcac agcaagaatt gcttcaatcc
     1861 aaaatggagc agctggggac tggagaacct ccagcagtac ctctgctgca agacgaccgg
     1921 cactccactt ccagtacaga gcaggagcgg gaaggaggac gaactccaac cctcgagatt
     1981 cttaaaagtc atttctccgg cattttccgg ccaaagtttt ccatacctcc ccctctccaa
     2041 ttggttcctg aggtccaaaa gccattgtac gaacagctgt ggtatcatgg tgctatcccc
     2101 agggctgagg tcgcagaatt gctgactcat tccggcgact ttcttgtgcg ggagtcccaa
     2161 ggtaagcagg aatatgttct gagcgttatg tgggacggtc aacccaggca ttttattatt
     2221 caatcctcag ataaccttta ccggcttgag ggcgacggct ttcccagcat tcctcttctg
     2281 attacacatt tgctgtcatc ccagcagccc ttgactaaaa aatctggggt cgtgcttttt
     2341 agggctgtgc ctaaggataa gtgggtcctc aaacacgaag acctggtctt gggagaacag
     2401 attggcaggg gaaatttcgg tgaggtgttc agtggcaggc ttcgagcaga caacacccct
     2461 gttgctgtta aaagttgccg agagactctg ccaccagacc ttaaggccaa gtttctccag
     2521 gaggcacgaa tcttgaaaca gtataatcac cctaacatcg tcaggctgat tggagtttgt
     2581 acccaaaaac aacctatata tattgttatg gaattggtgc aaggcggtga cttcctcacc
     2641 ttcctgcgga cagagggtgc tagactcagg gttaaaacct tgcttcaaat ggtaggagat
     2701 gcagctgctg gtatggaata cctcgagtct aaatgctgca ttcataggga cctggcagcc
     2761 aggaattgcc tggtgactga gaaaaacgtt ttgaaaatca gtgactttgg gatgtcccgg
     2821 gaagaggcag acgggattta tgctgcaagt gctggcctta gacaggtgcc cgtgaaatgg
     2881 accgctcctg agtgataagc ggccgcgttt aaacgtcgac ggtatcgata agcttgatat
     2941 cgaattccgc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     3001 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     3061 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     3121 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     3181 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     3241 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     3301 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     3361 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     3421 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     3481 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc
     3541 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3601 cacaagttca gcgtgtctgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3661 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3721 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3781 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3841 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3901 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3961 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggcgaac
     4021 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     4081 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     4141 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     4201 accgccgccg ggatcactct cggcatggac gagctgtaca agtaatgaat taattaagaa
     4261 ttcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     4321 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaatacataa
     4381 ctgagaatag agaagttcag atcaaggtta ggaacagaga gacagcagaa tatgggccaa
     4441 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc
     4501 agatgcggtc ccgccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca
     4561 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
     4621 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg
     4681 ccagtcctcc gatagactgc gtcgcccggg tacccgtgta tccaataaac cctcttgcag
     4741 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
     4801 ccgtcagcgg gggtctttca tgggtaacag tttcttgaag ttggagaaca acattctgag
     4861 ggtaggagtc gaatattaag taatcctgac tcaattagcc actgttttga atccacatac
     4921 tccaatactc ctgaaatagt tcattatgga cagcgcagaa agagctgggg agaattgtga
     4981 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     5041 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     5101 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     5161 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5221 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5281 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5341 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5401 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5461 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5521 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     5581 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     5641 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     5701 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     5761 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     5821 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     5881 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     5941 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6001 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6061 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6121 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6181 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     6241 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6301 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6361 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6421 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     6481 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     6541 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     6601 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     6661 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     6721 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     6781 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     6841 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     6901 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     6961 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7021 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7081 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     7141 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     7201 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     7261 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     7321 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     7381 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
     7441 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     7501 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     7561 aaaacgacgg cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca
     7621 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
     7681 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
     7741 tgatgccggc cacgatgcgt ccggcgtaga ggcgattagt ccaatttgtt aaagacagga
     7801 tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt
     7861 acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     7921 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     7981 tacataactg agaatagaga agttcagatc aaggttagga acagagagac agcagaatat
     8041 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     8101 ggtccccaga tgcggtcccg ccctcagcag tttctagaga accatcagat gtttccaggg
     8161 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
     8221 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
     8281 cggcgcgcca gtcctccgat agactgcgtc gcccgggtac ccgtattccc aataaagcct
     8341 cttgctgttt gcatccgaat cgtggactcg ctgatccttg ggagggtctc ctcagattga
     8401 ttgactgccc acctcggggg tctttcattt ggaggttcca ccgagatttg gagacccctg
     8461 cccagggacc accgaccccc ccgccgggag gtaagctggc cagcggtcgt ttcgtgtctg
     8521 tctctgtctt tgtgcgtgtt tgtgccggca tctaatgttt gcgcctgcgt ctgtactagt
     8581 tagctaacta gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg
     8641 gccgcaaccc tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag
     8701 gaagggagtc gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga
     8761 acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg
     8821 cgtcttgtct gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc
     8881 tgtatttgtc tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc
     8941 actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt
     9001 gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca
     9061 cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg
     9121 gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc
     9181 cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt
     9241 ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca
     9301 ctccttctct aggcgccgga att
//
