LOCUS       sequence-439172-        9555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible expression of GMIP mutant in mammalian cells by 
            retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Landau LM, Chaudhary N, Tien YC, Rogozinska M, Joshi S, Yao C, 
            Crowley J, Hullahalli K, Campbell IW, Waldor MK, Haigis M, Kagan JC
  TITLE     pLxIS-containing domains are biochemically flexible regulators of 
            interferons and metabolism.
  JOURNAL   Mol Cell. 2024 Jul 11;84(13):2436-2454.e10. doi: 
            10.1016/j.molcel.2024.05.030. Epub 2024 Jun 25.
  PUBMED    38925114
REFERENCE   2  (bases 1 to 9555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.05.030"; journalName: "Mol Cell"; date: 
            "2024-07-11- 11"; volume: "84"; issue: "13"; pages: "2436-2454.e10"
COMMENT     Sequence Label: pRetroX-TRE3G-GMIP S31D
FEATURES             Location/Qualifiers
     source          1..9555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..81
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        3005..3504
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3040..3063)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3437..3456
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3525..4124
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3525..3544)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4021..4041
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4305..4730
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(4991..5011)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4996..5325
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5176..5311
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5347..5366
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5447..5464)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5618..6206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5698..5717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6377..7168,7169..7237))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7000..7019
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7238..7342)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7410..7428
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        7528..7907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7908..8111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8112..8287
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    8350..8549
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             8750..9166
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     9096..9118
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     9134..9150
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        9206..9555
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    9214..9232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9250..9268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9286..9304
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9322..9340
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9358..9376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9394..9412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9430..9448
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     9494..9518
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggatccgcca ccatggacta caaggatcac gacggtgatt acaaggacca cgacatcgac
       61 tacaaggacg acgacgacaa gatggacagt gctgaaactg agctcacacc agcccccgag
      121 ggcaggaagc gatattctga tatatttcaa agtctcgaca atctggagat cgatctcgga
      181 aacgtgactt tcgaccccct tgcaggagat ccagtaagac gcgaagatct cgagcctgat
      241 aaggcagaca ccgccaccgt tgtgaccgag gagaacagtg aggcaagcag ttggcgcgac
      301 ttgagtccag agggcccagc cccccttaca gaagaggagc ttgatcttcg cttgatcagg
      361 accaaagggg gcgtggacgc cgcccttgaa tatgccaagg catggagtcg atacgcaaag
      421 gaactgctcg cttggacaga taaaagagca aactacgaac tcgagtttgc caaatcaata
      481 atgaagttgg cagaagctgg gaaagtatcc atattgcaac aatctcagat gcctttgcaa
      541 tacatctata ctttgtttct cgagcatgac ttgtccctcg gcgcattggc tttggaaact
      601 gtagcccaac aaaagcgcga ttactatcag cctcttgcag ccaaaaggat ggaaatcgaa
      661 aagtggagaa aagagttcaa ggaacaatgg ttgaaggagc aaaaaagaat gaatgaagca
      721 gtccaggcct tgagacgctc tgagctccag tacatacagc gacgcgagga cctgcgggca
      781 cgctcacagg gttctcctga ggaccctccc tcacaggcat cccccggctc caacaaacaa
      841 caagaaagac ggcggcgcag tagggaggaa gcacaggcta aggcccacga agccgaggca
      901 ctttaccagg catgtgtgag agaggctaat tcacgccaac aagatttgga aactacaaaa
      961 cggcggatcg tctcacatgt tcgcaagctc gtgctgcagg gggatgaagt actccgacgg
     1021 gttacacttg gacttttcga acttaggggg gctcaggcag agcggggacc tcgcagcttt
     1081 tccgctctgg ccgaatgttg cgtaccattc gaacccggtc aaagatatca agaatttgta
     1141 aggactctcc agccaggtgc ccctcctccc ccctcccctg ccttctgctt ccaagaattt
     1201 actgctgtag tccattcatt ccctcaggat acaaagaaga agttttctgg ccccctgccc
     1261 ccaaggcttg aagaggaagg aagtcccgag cccggtccat gggaggttgc aagcttgggt
     1321 tctcagggta ttccaggcag cgacgttgat tcagtagggg gaggctctga atctcgatcc
     1381 ttggattctc ctaccagttc tcccggcgca ggggccagaa ggctggtaaa ggccagttcc
     1441 acaggaaccg agtcctctga cgactttgaa gaacgggacc ccgaccttgg agatggaatc
     1501 gaaaatggtg taggcagtcc attcaggaag tggacattgt ctactgccgc acaaactcat
     1561 aggctgcgcc ggcttagagg tcccgcaaag tgcagggaat gtgaagcttt catggtttct
     1621 ggtaccgagt gcgaggaatg ttttttgacc tgccataagc gatgtttgga gacccttctt
     1681 atactgtgtg gccatcgacg ccttccagcc cgcatgtcac tctttggagt agacttcctc
     1741 cagctgccac gggactttcc tgaagaagtg cccttcgtca taacacggtg taccgctgag
     1801 atagaacata gggctttggg tcttcagggg atttacagag tctctgggtc ccgagtaagg
     1861 gttgaaagac tttgtcaagc cttcgaaaac gggcgagctt tggttgagct cagtggcaat
     1921 agtccccatg acattacaag cgtactcaaa aggtttctgc aggaactgac tgaccccgta
     1981 gttccattcc atctttatga tgcttttatc tctttggcaa agacactcca tgctgacccc
     2041 ggggacgatc caggtacccc aaacccatct cccgagatca tcagatcact gaaaacattg
     2101 cttgttcaac tccccgatag taattattcc accctccgcc acctcgtcgc acaccttttt
     2161 agagtcgctg ctcggttcga agaaaacaaa atgagcgcaa ataacctggg cattgtcttc
     2221 ggaccaacct tgctcaggcc cccagatggg cctcgagcaa ctggggctag ccccgtagcc
     2281 tgccttttgg acagcggcca ccaagcacag ctggtcgaat ttctcatcgt gcattacgag
     2341 caaatatttg gaatggacga gctgcctctt gcaagtgaac ctctgacaca ggaccccggc
     2401 ctggctccag cttgtttgga gtccagtccc caacaccctg catctctcct tgcccaagat
     2461 acccagccac ttacaattgc attggatagt agtccagacc ctaagcacca ttctgcactt
     2521 gagaaatgcc ccgaggttac accacccgaa ttggccacat tgcagcgaga tcagagggag
     2581 gaggaggttg aagacacacg cgacggtgct ggagatggct caagtcactg tcccgaagat
     2641 cttgcactgg gggcccagtc tcgaggtcac ttttcaaggc aaccagtaaa gtattcccgc
     2701 ggtggagtga ggcccgtgac ccaccaattg tcaagcctcg ctctcgtcgc ctcaaaactt
     2761 tgtgaagaga caccagttac cgtctccgca gtccacagag gtagtttgcg cgtaaggggc
     2821 ctgggccctg ccgccgcttg ccccgaggga agtcctcttc gccgcaaccc tctccctaaa
     2881 cattttgaga tcacacaaga aactgcacgc ttgctcagta aattgaattc agacgctgtc
     2941 tcccgcacca cttgttgcgc tgatccagaa cccgaggagt ccgaggagca cctctgataa
     3001 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     3061 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     3121 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     3181 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     3241 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     3301 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     3361 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     3421 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     3481 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc
     3541 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     3601 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     3661 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     3721 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     3781 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     3841 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     3901 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     3961 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     4021 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     4081 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     4141 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc ggcgtctcga gatatcagtg
     4201 gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca
     4261 tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     4321 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     4381 ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagggtc gaccctagag
     4441 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     4501 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     4561 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta
     4621 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
     4681 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg
     4741 tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc
     4801 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     4861 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     4921 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtag
     4981 atccggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5041 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     5101 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     5161 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5221 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     5281 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tactggctta
     5341 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     5401 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     5461 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5521 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5581 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5641 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5701 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5761 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5821 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5881 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5941 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6001 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6061 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6121 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6181 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6241 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6301 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6361 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6421 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6481 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6541 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6601 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6661 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     6721 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6781 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6841 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6901 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6961 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     7021 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7081 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7141 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7201 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7261 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7321 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     7381 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     7441 aagaattagc ttggccattg catacgttgt atccatatca taatatgtac atttatattg
     7501 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
     7561 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     7621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     7681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     7741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     7801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     7861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     7921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     7981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     8041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     8101 taagcagagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt cttccgatag
     8161 actgcgtcgc ccgggtaccc gtattcccaa taaagcctct tgctgtttgc atccgaatcg
     8221 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccac gacgggggtc
     8281 tttcatttgg gggctcgtcc gggatttgga gacccctgcc cagggaccac cgacccacca
     8341 ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgtt
     8401 tgatgttatg cgcctgcgtc tgtactagtt agctaactag ctctgtatct ggcggacccg
     8461 tggtggaact gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt
     8521 tgggggccgt ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc
     8581 aggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt
     8641 tttgctttcg gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt
     8701 tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt
     8761 taccactccc ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa
     8821 ccagtcggta gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac
     8881 ctttaacgtc ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa
     8941 gatcaaggtc ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac
     9001 ctgggaagcc ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc
     9061 tccgcctcct cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc
     9121 gcctcgatcc tccctttatc cagccctcac tccttctcta ggcgccggaa ttgaagatct
     9181 cgatgaggcc ctttcgtctt cactcgagtt tactccctat cagtgataga gaacgtatga
     9241 agagtttact ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata
     9301 gagaacgtat aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc
     9361 ctatcagtga tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat
     9421 ccagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggcg
     9481 cctataaaag cagagctcgt ttagtgaacc gtcagatcgc ctggagcaat tccacaacac
     9541 ttttgtctta tactt
//
