LOCUS       sequence-439211-        8880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible expression of FLAG-IRSp53-T2A-HA-Rac1 G12V in 
            mammalian cells by retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Landau LM, Chaudhary N, Tien YC, Rogozinska M, Joshi S, Yao C, 
            Crowley J, Hullahalli K, Campbell IW, Waldor MK, Haigis M, Kagan JC
  TITLE     pLxIS-containing domains are biochemically flexible regulators of 
            interferons and metabolism.
  JOURNAL   Mol Cell. 2024 Jul 11;84(13):2436-2454.e10. doi: 
            10.1016/j.molcel.2024.05.030. Epub 2024 Jun 25.
  PUBMED    38925114
REFERENCE   2  (bases 1 to 8880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.05.030"; journalName: "Mol Cell"; date: 
            "2024-07-11- 11"; volume: "84"; issue: "13"; pages: "2436-2454.e10"
COMMENT     Sequence Label: pRetroX-TRE3G-IRSp53-T2A-Rac1(G12V)
FEATURES             Location/Qualifiers
     source          1..8880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..39
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1654..1707
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1708..1737
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        2330..2829
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2365..2388)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2762..2781
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2850..3449
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2850..2869)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3346..3366
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3630..4055
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(4158..4180)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4316..4336)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4321..4650
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4501..4636
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4672..4691
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4772..4789)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4943..5531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5023..5042)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5702..6493,6494..6562))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6325..6344
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6563..6667)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6735..6753
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        6853..7232
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7233..7436
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7437..7612
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    7675..7874
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             8075..8491
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     8421..8443
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     8459..8475
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        8531..8880
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    8539..8557
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8575..8593
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8611..8629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8647..8665
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8683..8701
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8719..8737
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8755..8773
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     8819..8843
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggatccgcca ccatggatta caaagacgac gacgacaaga tgtccctctc tcgaagtgag
       61 gagatgcaca ggttgaccga aaacgtgtac aaaaccatca tggagcaatt caaccccagt
      121 ctcagaaact tcatagctat gggcaaaaat tacgaaaagg cactcgcagg tgtgactttt
      181 gccgcaaaag ggtattttga cgcacttgtt aaaatgggag aacttgctag cgaatcccag
      241 gggtctaagg agctgggtga tgttctcttc caaatggctg aagttcacag gcagatacag
      301 aaccaactgg aggagaccct caagagtttt cataatgaat tgcttactca gttggaacaa
      361 aaagttgaat tggattctcg ctacctcagt gcagcactca agaagtatca gaccgagcag
      421 cggtctaaag gagatgcatt ggataaatgt caagcagaac ttaagaagct gcggaagaag
      481 tcccaaggta gcaaaaatcc ccagaaatac tctgacaagg agctgcagta tatcgatgcc
      541 atatcaaata aacaaggcga gctcgaaaat tatgtatcag atgggtataa aactgctttg
      601 accgaggagc gcaggcggtt ctgctttctt gtcgagaaac agtgtgctgt cgccaagaat
      661 agcgctgctt atcatagtaa gggtaaagaa ttgctggcac agaaactccc actttggcag
      721 caagcatgcg ccgaccccaa caagataccc gacagggctg ttcaacttat gcagcagatg
      781 gccaactcta acggctccat cctccccagc gctttgtcag cctcaaaatc aaacttggta
      841 attagcgatc ccattcctgg tgccaagcca ttgcccgtcc cacctgagct cgccccattc
      901 gtcggtcgaa tgtcagctca agagaatgta ccagtcatga acggagtggc cggcccagat
      961 tctgaggatt ataatccatg ggccgaccgg aaagcagctc aacccaaaag cctgtctcca
     1021 ccacaatccc aaagcaagct tagcgattca tattccaaca ctttgcctgt aagaaagtca
     1081 gttacaccta agaacagcta tgccactaca gaaaataaaa ccctgccaag aagcagttct
     1141 atggccgctg gccttgagcg caatggccgg atgagagtaa aagccatttt cagccacgcc
     1201 gccggggaca acagcaccct tctgagtttt aaggaagggg atttgattac tcttctcgtg
     1261 cccgaggcca gggatggttg gcattatggt gaatcagaaa aaaccaagat gaggggctgg
     1321 ttccccttct cttatacccg ggtgcttgat tctgatggga gcgataggtt gcacatgagc
     1381 ctccaacagg gtaagagctc atcaacagga aatctcttgg ataaagatga tttggctttg
     1441 cctccaccag actacggtac aagtagccga gcatttccaa cccagacagc tggcaccttt
     1501 aagcaacggc catatagcgt agcagtccca gccttttctc agggtcttga tgattatggc
     1561 gcaagatcag tttctaggaa tccattcgcc aacgtacacc tgaagccaac cgtgacaaac
     1621 gacaggtctg ccccccttct gagtggctcc ggggaaggga gaggtagcct tcttacctgt
     1681 ggagacgttg aggagaatcc cggacccgag caaaaactca tttcagagga agatcttatg
     1741 caggcaatta agtgtgttgt ggtgggagac gtagccgtag ggaaaacctg tctccttatc
     1801 tcctatacca ccaatgcatt cccaggcgag tacatcccaa ctgttttcga caactattca
     1861 gccaacgtaa tggtggatgg taaaccagta aatctcgggc tttgggacac tgctgggcaa
     1921 gaggactacg accgattgag acctctgtcc tatccccaaa ccgatgtgtt tttgatatgt
     1981 ttcagtctgg tcagtccagc tagctttgag aacgtgcgag caaaatggta cccagaggtt
     2041 cgccatcact gtcctaacac acccatcata ctcgtgggga ctaaactcga cttgcgggat
     2101 gataaagaca ctatagaaaa acttaaggaa aaaaagctca cccctattac ctatcctcag
     2161 ggactggcca tggccaagga gatcggcgct gtaaaatacc tcgaatgttc cgctttgaca
     2221 caacgcggcc tgaagaccgt attcgatgag gccattaggg cagttctttg tccaccaccc
     2281 gtgaaaaaaa gaaaaaggaa atgtttgctc ctgtgataag aattctaccg ggtaggggag
     2341 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     2401 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     2461 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     2521 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     2581 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     2641 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     2701 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     2761 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     2821 ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg
     2881 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     2941 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     3001 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     3061 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     3121 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     3181 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     3241 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     3301 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     3361 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     3421 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg
     3481 aaaggagcgc acgaccccat gcatcggcgt ctcgagatat cagtggtcca ggctctagtt
     3541 ttgactcaac aatatcacca gctgaagcct atagagtacg agccatagat aaaataaaag
     3601 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     3661 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag
     3721 ttcagatcaa ggtcaggaac agatggaaca gggtcgaccc tagagaacca tcagatgttt
     3781 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     3841 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     3901 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
     3961 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
     4021 agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga
     4081 cccctgccca gggaccaccg acccaccacc gggaggtaag ctggctgcct cgcgcgtttc
     4141 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     4201 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     4261 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtagatccg gctgtggaat
     4321 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4381 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     4441 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4501 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4561 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     4621 ggcttttttg gaggcctagg cttttgcaaa aagcttactg gcttaactat gcggcatcag
     4681 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     4741 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4801 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4861 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4921 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4981 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5041 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5101 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5161 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5221 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     5281 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     5341 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     5401 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5461 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5521 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5581 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5641 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5701 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5761 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5821 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5881 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5941 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6001 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6061 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6121 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6181 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6241 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6301 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6361 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6421 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6481 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6541 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6601 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6661 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     6721 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttagcttggc
     6781 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat
     6841 taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     6901 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     6961 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     7021 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     7081 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     7141 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     7201 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     7261 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     7321 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     7381 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcaat
     7441 aaaagagccc acaacccctc actcggcgcg ccagtcttcc gatagactgc gtcgcccggg
     7501 tacccgtatt cccaataaag cctcttgctg tttgcatccg aatcgtggtc tcgctgttcc
     7561 ttgggagggt ctcctctgag tgattgacta cccacgacgg gggtctttca tttgggggct
     7621 cgtccgggat ttggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
     7681 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgtttgatg ttatgcgcct
     7741 gcgtctgtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
     7801 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
     7861 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
     7921 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
     7981 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
     8041 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
     8101 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     8161 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     8221 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     8281 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     8341 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     8401 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     8461 ttatccagcc ctcactcctt ctctaggcgc cggaattgaa gatctcgatg aggccctttc
     8521 gtcttcactc gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta
     8581 tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga
     8641 gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag
     8701 aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta
     8761 tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt gggcgcctat aaaagcagag
     8821 ctcgtttagt gaaccgtcag atcgcctgga gcaattccac aacacttttg tcttatactt
//
