LOCUS       sequence-439234-         498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of N-terminally 6His-tagged hnRNPA1_YtoW.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Alshareedah I, Borcherds WM, Cohen SR, Singh A, Posey AE, Farag M, 
            Bremer A, Strout GW, Tomares DT, Pappu RV, Mittag T, Banerjee PR
  TITLE     Sequence-specific interactions determine viscoelasticity and aging 
            dynamics of protein condensates.
  JOURNAL   Nat Phys. 2024 Sep;20(9):1482-1491. doi: 10.1038/s41567-024-02558-1.
            Epub 2024 Jul 2.
  PUBMED    39464253
REFERENCE   2  (bases 1 to 498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41567-024-02558-1"; journalName: "Nat Phys"; date: 
            "2024-09"; volume: "20"; issue: "9"; pages: "1482-1491"
COMMENT     Sequence Label: pDest17_hnRNPA1_YtoW
FEATURES             Location/Qualifiers
     source          1..498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac ctcgaatcaa caagtttgta caaaaaagca
       61 ggcttcgaga acctgtattt tcagggtagc atggcaagcg caagcagcag ccagcgtggt
      121 cgtagcggta gcggtaattt tggtggtggt cgcggtggtg gttttggtgg caatgataat
      181 tttggccgtg gtggtaattt tagcggtcgt ggtggctttg gcggtagccg tggcggaggt
      241 ggttggggtg gtagtggtga tggttggaat ggctttggta atgatggtag caactttggt
      301 ggcggtggta gctggaatga ttttggcaat tggaataatc agagcagcaa tttcggtccg
      361 atgaaaggtg gcaattttgg aggtcgtagc agcggtggtt caggtggtgg tggccagtgg
      421 tttgcaaaac cgcgtaatca aggtggctgg ggaggttcta gcagcagcag ctcatggggt
      481 agtggtcgtc gtttttaa
//
