LOCUS       sequence-439235-         498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of N-terminally 6His-tagged hnRNPA1_FtoW.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Alshareedah I, Borcherds WM, Cohen SR, Singh A, Posey AE, Farag M, 
            Bremer A, Strout GW, Tomares DT, Pappu RV, Mittag T, Banerjee PR
  TITLE     Sequence-specific interactions determine viscoelasticity and aging 
            dynamics of protein condensates.
  JOURNAL   Nat Phys. 2024 Sep;20(9):1482-1491. doi: 10.1038/s41567-024-02558-1.
            Epub 2024 Jul 2.
  PUBMED    39464253
REFERENCE   2  (bases 1 to 498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41567-024-02558-1"; journalName: "Nat Phys"; date: 
            "2024-09"; volume: "20"; issue: "9"; pages: "1482-1491"
COMMENT     Sequence Label: pDest17_hnRNPA1_FtoW
FEATURES             Location/Qualifiers
     source          1..498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac ctcgaatcaa caagtttgta caaaaaagca
       61 ggcttcgaga acctgtattt tcagggtagc atggcaagcg caagcagcag ccagcgtggt
      121 cgtagcggta gcggtaattg gggtggtggt cgcggtggtg gttggggtgg caatgataac
      181 tggggtcgtg gtggtaattg gagcggtcgc ggaggctggg gtggttcaag aggtggtggt
      241 ggctatggtg gtagtggtga tggttataat ggttggggca atgatggtag caactggggt
      301 ggcggtggca gctataacga ttggggtaat tacaataatc agagcagcaa ttggggtccg
      361 atgaaaggtg gtaactgggg aggtcgtagc agcggtggtt caggtggtgg cggtcagtat
      421 tgggcaaaac cgcgtaatca aggtggttat ggcggtagca gcagcagctc aagctatggt
      481 agcggtcgtc gttggtaa
//
