LOCUS       sequence-439249-        9274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dCas9-FCPF in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Altinbay M, Wang J, Chen J, Schafer D, Sprang M, Blagojevic B, Wolfl
            S, Andrade-Navarro MA, Dikic I, Knapp S, Cheng X
  TITLE     Chem-CRISPR/dCas9FCPF: a platform for chemically induced epigenome 
            editing.
  JOURNAL   Nucleic Acids Res. 2024 Sep 24:gkae798. doi: 10.1093/nar/gkae798.
  PUBMED    39315698
REFERENCE   2  (bases 1 to 9274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 Sep 24:gkae798. doi: 10.1093/nar/gkae798."
COMMENT     Sequence Label: pET-dCas9-FCPF
FEATURES             Location/Qualifiers
     source          1..9274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2755..2775
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2797..2814
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2885..2903)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2899..2946
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2983..3438
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3070..3089)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3280..3301
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(3553..4344,4345..4413))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4176..4195
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4414..4506)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4587..5175
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5076..5095
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5329..5346
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5361..5500
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5426..5445)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5586..5608
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5602..5793)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6365..7447)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7409..7428
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7448..7525)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     7731..7750
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     7838..7857
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7838..7856
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7857..7881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7896..7918
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(7925..7951,7952..7954,7955..9274,1..1167,1168..1170,
                     1171..2754)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 tatgttggtc cattggcgcg tggcaatagt cgttttgcat ggatgactcg gaagtctgaa
       61 gaaacaatta ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc agctcaatca
      121 tttattgaac gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt actaccaaaa
      181 catagtttgc tttatgagta ttttacggtt tataacgaat tgacaaaggt caaatatgtt
      241 actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc cattgttgat
      301 ttactcttca aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa
      361 aaaatagaat gttttgatag tgttgaaatt tcaggagttg aagatagatt taatgcttca
      421 ttaggtacct accatgattt gctaaaaatt attaaagata aagatttttt ggataatgaa
      481 gaaaatgaag atatcttaga ggatattgtt ttaacattga ccttatttga agatagggag
      541 atgattgagg aaagacttaa aacatatgct cacctctttg atgataaggt gatgaaacag
      601 cttaaacgtc gccgttatac tggttgggga cgtttgtctc gaaaattgat taatggtatt
      661 agggataagc aatctggcaa aacaatatta gattttttga aatcagatgg ttttgccaat
      721 cgcaatttta tgcagctgat ccatgatgat agtttgacat ttaaagaaga cattcaaaaa
      781 gcacaagtgt ctggacaagg cgatagttta catgaacata ttgcaaattt agctggtagc
      841 cctgctatta aaaaaggtat tttacagact gtaaaagttg ttgatgaatt ggtcaaagta
      901 atggggcggc ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact
      961 caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa
     1021 ttaggaagtc agattcttaa agagcatcct gttgaaaata ctcaattgca aaatgaaaag
     1081 ctctatctct attatctcca aaatggaaga gacatgtatg tggaccaaga attagatatt
     1141 aatcgtttaa gtgattatga tgtcgatgcc attgttccac aaagtttcct taaagacgat
     1201 tcaatagaca ataaggtctt aacgcgttct gataaaaatc gtggtaaatc ggataacgtt
     1261 ccaagtgaag aagtagtcaa aaagatgaaa aactattgga gacaacttct aaacgccaag
     1321 ttaatcactc aacgtaagtt tgataattta acgaaagctg aacgtggagg tttgagtgaa
     1381 cttgataaag ctggttttat caaacgccaa ttggttgaaa ctcgccaaat cactaagcat
     1441 gtggcacaaa ttttggatag tcgcatgaat actaaatacg atgaaaatga taaacttatt
     1501 cgagaggtta aagtgattac cttaaaatct aaattagttt ctgacttccg aaaagatttc
     1561 caattctata aagtacgtga gattaacaat taccatcatg cccatgatgc gtatctaaat
     1621 gccgtcgttg gaactgcttt gattaagaaa tatccaaaac ttgaatcgga gtttgtctat
     1681 ggtgattata aagtttatga tgttcgtaaa atgattgcta agtctgagca agaaataggc
     1741 aaagcaaccg caaaatattt cttttactct aatatcatga acttcttcaa aacagaaatt
     1801 acacttgcaa atggagagat tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga
     1861 gaaattgtct gggataaagg gcgagatttt gccacagtgc gcaaagtatt gtccatgccc
     1921 caagtcaata ttgtcaagaa aacagaagta cagacaggcg gattctccaa ggagtcaatt
     1981 ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa aagactggga tccaaaaaaa
     2041 tatggtggtt ttgatagtcc aacggtagct tattcagtcc tagtggttgc taaggtggaa
     2101 aaagggaaat cgaagaagtt aaaatccgtt aaagagttac tagggatcac aattatggaa
     2161 agaagttcct ttgaaaaaaa tccgattgac tttttagaag ctaaaggata taaggaagtt
     2221 aaaaaagact taatcattaa actacctaaa tatagtcttt ttgagttaga aaacggtcgt
     2281 aaacggatgc tggctagtgc cggagaatta caaaaaggaa atgagctggc tctgccaagc
     2341 aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg tagtccagaa
     2401 gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga tgagattatt
     2461 gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt agataaagtt
     2521 cttagtgcat ataacaaaca tagagacaaa ccaatacgtg aacaagcaga aaatattatt
     2581 catttattta cgttgacgaa tcttggagct cccgctgctt ttaaatattt tgatacaaca
     2641 attgatcgta aacgatatac gtctacaaaa gaagttttag atgccactct tatccatcaa
     2701 tccatcactg gtctttatga aacacgcatt gatttgagtc agctaggagg tgaccccaag
     2761 aagaagagga aggtgttctg cccattcatg gataagcatc accaccacca tcactaatga
     2821 taatttgaac gccagcacat ggactcgtct actagcgcag cttaattaac ctaggctgct
     2881 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
     2941 tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg
     3001 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3061 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3121 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3181 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3241 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3301 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3361 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     3421 tattaacgtt tacaatttct ggcggcacga tggcatgaga ttatcaaaaa ggatcttcac
     3481 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3541 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3601 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3661 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3721 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3781 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3841 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3901 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3961 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     4021 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     4081 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4141 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     4201 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4261 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4321 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4381 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat catgattgaa
     4441 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4501 aacaaatagg tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4561 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4621 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4681 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     4741 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4801 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4861 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4921 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4981 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5041 gtcggaacag gagagcgcac gagggagctc ccagggggaa acgcctggta tctttatagt
     5101 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5161 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5221 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5281 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5341 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     5401 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     5461 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     5521 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     5581 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     5641 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     5701 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     5761 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     5821 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     5881 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     5941 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     6001 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     6061 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt
     6121 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
     6181 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
     6241 cacgatcatg ctagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
     6301 caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt
     6361 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6421 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
     6481 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
     6541 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
     6601 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatgtcc gcaccaacgc
     6661 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
     6721 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca
     6781 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
     6841 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
     6901 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
     6961 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
     7021 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
     7081 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
     7141 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
     7201 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
     7261 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
     7321 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
     7381 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
     7441 gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa
     7501 aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc
     7561 attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt
     7621 gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
     7681 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
     7741 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
     7801 gcgtagagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac tataggggaa
     7861 ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggagata
     7921 taccatggat aagaaatact caataggctt agccatcggc acaaatagcg tcggatgggc
     7981 ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga
     8041 ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc
     8101 ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat
     8161 ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca
     8221 tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt
     8281 tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg
     8341 aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc
     8401 gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag
     8461 tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa
     8521 ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc
     8581 aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg
     8641 gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc
     8701 agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt
     8761 ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc
     8821 tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc
     8881 ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt
     8941 tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata
     9001 tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat
     9061 tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct
     9121 gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact tgggtgagct
     9181 gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa
     9241 gattgaaaaa atcttgactt ttcgaattcc ttat
//
