LOCUS       sequence-439267-       11190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human integrin alpha5 ectodomain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11190)
  AUTHORS   Li J, Jo MH, Yan J, Hall T, Lee J, Lopez-Sanchez U, Yan S, Ha T, 
            Springer TA
  TITLE     Ligand binding initiates single-molecule integrin conformational 
            activation.
  JOURNAL   Cell. 2024 May 14:S0092-8674(24)00475-6. doi: 
            10.1016/j.cell.2024.04.049.
  PUBMED    38772370
REFERENCE   2  (bases 1 to 11190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            May 14:S0092-8674(24)00475-6. doi: 10.1016/j.cell.2024.04.049."
COMMENT     Sequence Label: PD2529 human alpha5 ecto
FEATURES             Location/Qualifiers
     source          1..11190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(14..513)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(62..81)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     455..478
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     enhancer        597..900
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        902..1177
                     /label=chicken beta-actin promoter
     intron          1178..2194
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2202..2221
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5224..5247
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5302..5313
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5338..5373
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             join(5374..5379,5380..5403,5404..5439,5440..5463)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             5485..5541
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    7855..7910
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7909..7928)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(8620..9208)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8700..8719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9294..10103)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10017..10036
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(10122..10169)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             complement(join(10595..11190,1..4))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10678..10698)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     join(11175..11190,1..4)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tcatggtggc ggacgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc
       61 gcttttgaag cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc
      121 tgagcccgcc cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag
      181 cgaaggagca aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag
      241 cggtgctgtc catctgcacg agactagtga gtcgtgctac ttccatttgt cacgtcctgc
      301 acgacgcgag ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg
      361 cgaaggggcc accaaagaac ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc
      421 gaggccagag gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc
      481 cagactgcct tgggaaaagc gcctccccta cccggtagag aaacttgatc tgtcgccgca
      541 attcaaactt cgtgaggctc cggtgcccgt cagtgacctg ctatactctg gagacgcgtt
      601 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      661 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      721 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      781 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      841 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      901 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
      961 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
     1021 gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc
     1081 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
     1141 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg
     1201 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
     1261 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
     1321 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
     1381 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
     1441 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
     1501 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
     1561 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     1621 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
     1681 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
     1741 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
     1801 ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg
     1861 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
     1921 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa
     1981 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
     2041 ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg
     2101 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
     2161 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
     2221 gctgtctcat cattttggca aagaattcgg cttgatcgaa gccgtctcag gggagagacc
     2281 acacccaagc tgtctagagc cgccaccatg cctttgctcc tcctgttgcc cttgctctgg
     2341 gctggcgccc tcgcgttcaa cttagacgcg gaggccccag cagtactctc ggggcccccg
     2401 ggctccttct tcggattctc agtggagttt taccggccgg gaacagacgg ggtcagcgtg
     2461 ctggtgggag cacccaaggc taataccagc cagccaggag tgctgcaggg tggtgctgtc
     2521 tacctctgtc cttggggtgc cagccccaca cagtgcaccc ccattgaatt tgacagcaaa
     2581 ggctctcggc tcctggagtc ctcactgtcc agctcagagg gagaggagcc tgtggagtac
     2641 aagtccttgc agtggttcgg ggcaacagtt cgagcccatg gctcctccat cttggcatgc
     2701 gctccactgt acagctggcg cacagagaag gagccactga gcgaccccgt gggcacctgc
     2761 tacctctcca cagataactt cacccgaatt ctggagtatg caccctgccg ctcagatttc
     2821 agctgggcag caggacaggg ttactgccaa ggaggcttca gtgccgagtt caccaagact
     2881 ggccgtgtgg ttttaggtgg accaggaagc tatttctggc aaggccagat cctgtctgcc
     2941 actcaggagc agattgcaga atcttattac cccgagtacc tgatcaacct ggttcagggg
     3001 cagctgcaga ctcgccaggc cagttccatc tatgatgaca gctacctagg atactctgtg
     3061 gctgttggtg aattcagtgg tgatgacaca gaagactttg ttgctggtgt gcccaaaggg
     3121 aacctcactt acggctatgt caccatcctt aatggctcag acattcgatc cctctacaac
     3181 ttctcagggg aacagatggc ctcctacttt ggctatgcag tggccgccac agacgtcaat
     3241 ggggacgggc tggatgactt gctggtgggg gcacccctgc tcatggatcg gacccctgac
     3301 gggcggcctc aggaggtggg cagggtctac gtctacctgc agcacccagc cggcatagag
     3361 cccacgccca cccttaccct cactggccat gatgagtttg gccgatttgg cagctccttg
     3421 acccccctgg gggacctgga ccaggatggc tacaatgatg tggccatcgg ggctcccttt
     3481 ggtggggaga cccagcaggg agtagtgttt gtatttcctg ggggcccagg agggctgggc
     3541 tctaagcctt cccaggttct gcagcccctg tgggcagcca gccacacccc agacttcttt
     3601 ggctctgccc ttcgaggagg ccgagacctg gatggcaatg gatatcctga tctgattgtg
     3661 gggtcctttg gtgtggacaa ggctgtggta tacaggggcc gccccatcgt gtccgctagt
     3721 gcctccctca ccatcttccc cgccatgttc aacccagagg agcggagctg cagcttagag
     3781 gggaaccctg tggcctgcat caaccttagc ttctgcctca atgcttctgg aaaacacgtt
     3841 gctgactcca ttggtttcac agtggaactt cagctggact ggcagaagca gaagggaggg
     3901 gtacggcggg cactgttcct ggcctccagg caggcaaccc tgacccagac cctgctcatc
     3961 cagaatgggg ctcgagagga ttgcagagag atgaagatct acctcaggaa cgagtcagaa
     4021 tttcgagaca aactctcgcc gattcacatc gctctcaact tctccttgga cccccaagcc
     4081 ccagtggaca gccacggcct caggccagcc ctacattatc agagcaagag ccggatagag
     4141 gacaaggctc agatcttgct ggactgtgga gaagacaaca tctgtgtgcc tgacctgcag
     4201 ctggaagtgt ttggggagca gaaccatgtg tacctgggtg acaagaatgc cctgaacctc
     4261 actttccatg cccagaatgt gggtgagggt ggcgcctatg aggctgagct tcgggtcacc
     4321 gcccctccag aggctgagta ctcaggactc gtcagacacc cagggaactt ctccagcctg
     4381 agctgtgact actttgccgt gaaccagagc cgcctgctgg tgtgtgacct gggcaacccc
     4441 atgaaggcag gagccagtct gtggggtggc cttcggttta cagtccctca tctccgggac
     4501 actaagaaaa ccatccagtt tgacttccag atcctcagca agaatctcaa caactcgcaa
     4561 agcgacgtgg tttcctttcg gctctccgtg gaggctcagg cccaggtcac cctgaacggt
     4621 gtctccaagc ctgaggcagt gctattccca gtaagcgact ggcatccccg agaccagcct
     4681 cagaaggagg aggacctggg acctgctgtc caccatgtct atgagctcat caaccaaggc
     4741 cccagctcca ttagccaggg tgtgctggaa ctcagctgtc cccaggctct ggaaggtcag
     4801 cagctcctat atgtgaccag agttacggga ctcaactgca ccaccaatca ccccattaac
     4861 ccaaagggcc tggagttgga tcccgagggt tccctgcacc accagcaaaa acgggaagct
     4921 ccaagccgca gctctgcttc ctcgggacct cagatcctga aatgcccgga ggctgagtgt
     4981 ttcaggctgc gctgtgagct cgggcccctg caccaacaag agagccaaag tctgcagttg
     5041 catttccgag tctgggccaa gactttcttg cagcgggagc accagccatt tagcctgcag
     5101 tgtgaggctg tgtacaaagc cctgaagatg ccctaccgaa tcctgcctcg gcagctgccc
     5161 caaaaagagc gtcaggtggc cacagctgtg caatggacca aggcagaagg cagctatgct
     5221 agccttgagg tgcttttcca aggtcccgca caatgcgaga aagagttgca agcccttgaa
     5281 aaggagaacg ctcaacttga gtgggaactt caagccctcg aaaaggagct tgcccaagag
     5341 gaccaagtag acccgcggct gatagatggt aaaagtgcat ggtctcatcc ccaatttgaa
     5401 aaaggtgggg ggtcaggcgg cggttcaggc gggagtgctt ggtcacaccc ccaatttgag
     5461 aaaggtacca gtactgggag tggggccacc aacttttctc tgctcaagca agccggggat
     5521 gttgaagaaa atccagggcc aatggtgtct aagggcgcag aactgttcac cggaatcgta
     5581 cctattttga tagaacttaa tggggatgtc aatggacata aattcagtgt cagcggtgag
     5641 ggtgaaggcg atgcgactta tgggaaactg acgctcaaat ttatctgtac caccggtaaa
     5701 ctccccgttc cgtggccgac ccttgtcacc accctgtcct atggcgttca gtgttttagc
     5761 aggtatccag atcacatgaa acagcatgac tttttcaagt ccgccatgcc tgagggttac
     5821 attcaagagc gaacgatatt ctttgaagac gatggcaact ataagtccag ggcggaggtc
     5881 aagtttgagg gcgacacgct tgtgaatagg attgagctta cggggactga ttttaaagaa
     5941 gacggcaata tccttgggaa taaaatggaa tacaactata atgcccacaa cgtgtatata
     6001 atgacggata aggccaagaa cggaatcaaa gtcaacttta aaatcagaca caacatcgaa
     6061 gatggcagcg tgcaacttgc cgaccattac cagcaaaaca cgcctattgg cgacggtcct
     6121 gtcttgctcc ctgacaatca ctatctcagc acgcaaagtg ccctgagtaa agatcctaac
     6181 gagaaacggg atcacatgat ttactttggg tttgtgacag cggctgctat aacgcatggt
     6241 atggacgaat tgtacaaatg ataaagtact tgataagctc ttcaggttga ggtctctaaa
     6301 agcgtcttcc tgttctcatc acatcatatc aaggttatat accatcaata ttgccacaga
     6361 tgttacttag ccttttaata tttctctaat ttagtgtata tgcaatgata gttctctgat
     6421 ttctgagatt gagtttctca tgtgtaatga ttatttagag tttctctttc atctgttcaa
     6481 atttttgtct agttttattt tttactgatt tgtaagactt ctttttataa tctgcatatt
     6541 acaattctct ttactggggt gttgcaaata ttttctgtca ttctatggcc tgacttttct
     6601 taatggtttt ttaattttaa aaataagtct taatattcat gcaatctaat taacaatctt
     6661 ttctttgtgg ttaggacttt gagtcataag aaatttttct ctacactgaa gtcatgatgg
     6721 catgcttcta tattattttc taaaagattt aaagttttgc cttctccatt tagacttata
     6781 attcactgga atttttttgt gtgtatggta tgacatatgg gttccctttt attttttaca
     6841 tataaatata tttccctgtt tttctaaaaa agaaaaagat catcattttc ccattgtaaa
     6901 atgccatatt tttttcatag gtcacttaca tatatcaatg ggtctgtttc tgagctctac
     6961 tctattttat cagcctcact gtctatcccc acacatctca tgctttgctc taaatcttga
     7021 tatttagtgg aacattcttt cccattttgt tctacaagaa tatttttgtt attgtctttg
     7081 ggctttctat atacattttg aaatgaggtt gacaagttaa taacaggcct attgattgga
     7141 aagtttgtca acgaattgtg ggtcttttgg ggtttgctgc cccttttacg caatgtggat
     7201 atcctgcttt aatgccttta tatgcatgta tacaagcaaa acaggctttt actttctcgc
     7261 caacttacaa ggcctttctc agtaaacagt atatgaccct ttaccccgtt gctcggcaac
     7321 ggcctggtct gtgccaagtg tttgctgacg caacccccac tggttggggc ttggccatag
     7381 gccatcagcg catgcgtgga acctttgtgt ctcctctgcc gatccatact gcggaactcc
     7441 tagccgcttg ttttgctcgc agcaggtctg gagcaaacct catcgggacc gacaattctg
     7501 tcgtactctc ccgcaagtat acatcgtttc catggctgct aggctgtgct gccaactgga
     7561 tcctgcgcgg gacgtccttt gtttacgtcc cgtcggcgct gaatcccgcg gacgacccct
     7621 cccggggccg cttggggctc taccgcccgc ttctccgtct gccgtaccgt ccgaccacgg
     7681 ggcgcacctc tctttacgcg gactccccgt ctgtgccttc tcatctgccg gaccgtgtgc
     7741 acttcgcttc acctctgcac gtcgcatgga ggccaccgtg aacgcccacc ggaacctgcc
     7801 caaggtcttg cataagagga ctcttggact ttcagcaatg tcatctcagt ggctaataaa
     7861 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     7921 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     7981 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     8041 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     8101 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     8161 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     8221 ttctcttatg gagatctttg catttttaga catttagaag cctatatctt gttacagaat
     8281 tggaattaca caaaaattct accatatttt gaaagcttag gttgttctga aaaaaacaat
     8341 atattgtttt cctgggtaaa ctaaaagtcc cctcgaggaa aggcccctaa agtgaaacag
     8401 tgcaaaacgt tcaaaaactg tctggcaata caagttccac tttgaccaaa acggctggca
     8461 gtaaaagggt taagaagact gtcagccttg agcggtatca gctcactcaa aggcggtaat
     8521 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8581 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8641 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8701 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8761 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8821 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8881 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8941 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     9001 gtatgtaggc ggtgctacag agttcttgaa gtggtgggct aactacggct acactagaag
     9061 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     9121 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9181 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9241 cgctcagtgg aacgacgcgc gcgtaactca cgttaaggga ttttggtcat gagttagaaa
     9301 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     9361 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     9421 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     9481 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     9541 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
     9601 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     9661 gcgaggcgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgagtgcaac
     9721 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     9781 aatacctgga acgctgtttt tccggggatc gcagtggtga gtaaccatgc atcatcagga
     9841 gtacggataa aatgcttgat ggtcggaagt ggcataaatt ccgtcagcca gtttagtctg
     9901 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     9961 ggcgcatcgg gcttcccata caagcgatag attgtcgcac ctgattgccc gacattatcg
    10021 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctcgac
    10081 gtttcccgtt ggatatggct cattttttac ttcctcacct tgtcgtatta tactatgccg
    10141 atatactatg ccgatgatta attgtcgaca ctgcgggggc tctgtgtggt aagcaggtct
    10201 taaccttttt actgccaatg acgcatggga tacgtcgtgg cagtaaaagg gcttaaatgc
    10261 caacgacgcg tcccatacgt tgttggcatt ttaattcttc tctctgcagc ggcagcatgt
    10321 gccgccgctg cagagagttt ctagcgatga cagcccctct gggcaacgag ccgggggggc
    10381 tgtctttctt tatgttttaa atgcactgac ctcccacatt ccctttttag taaaatattc
    10441 agaaataatt taaatacatc attgcaatga aaataaatgt tttttattag gcagaatcca
    10501 gatgctcaag gcccttcata atatccccca gtttagtagt tggacttagg gaacaaagga
    10561 acctttaata gaaattggac agcaagaaag cgagtcaggc accgggcttg cgggtcatgc
    10621 accaggtgcg cggtccttcg ggcacctcga cgtcggcggt gacggtgaag ccgagccgct
    10681 cgtagaaggg gaggttgcgg ggcgcggatg tctccaggaa ggcgggcacc ccggcgcgct
    10741 cggccgcctc cactccgggg agcacgacgg cgctgcccag acccttgccc tggtggtcgg
    10801 gcgacacgcc gacggtggcc aggaaccacg cgggctcctt gggccggtgc ggcgccagga
    10861 ggccttccat ctgttgctgc gcggccagcc gggaaccgct caactcggcc atgcgcgggc
    10921 cgatctcggc gaacaccgcc cccgcttcga cgctctccgg cgtggtccag accgccaccg
    10981 cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga
    11041 gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg atcgacggtg tggcgcgtgg
    11101 cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc cctggggacg tcgtcgcggg
    11161 tggcgaggcg caccgtgggc ttgtactcgg
//
