LOCUS       sequence-43932-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Zariwala HA, Borghuis BG, Hoogland TM, Madisen L, Tian L, De Zeeuw 
            CI, Zeng H, Looger LL, Svoboda K, Chen TW
  TITLE     A Cre-dependent GCaMP3 reporter mouse for neuronal imaging in vivo.
  JOURNAL   J Neurosci. 2012 Feb 29;32(9):3131-41. doi: 
            10.1523/JNEUROSCI.4469-11.2012.
  PUBMED    22378886
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.4469-11"; journalName: "J Neurosci"; date: 
            "2012-02-29- 29"; volume: "32"; issue: "9"; pages: "3131-41"
COMMENT     Sequence Label: Ai38
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(67..88)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(301..357)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      369..378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(385..399,400..408))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(412..444)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(448..465)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      471..480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(598..631)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    660..794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(684..703)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     738..757
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 taccatgctc cctccggtac cgcccttgta cagctcgtcc atgccgagag tgatcccggc
       61 ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttcgaaag
      121 tttggactgc acgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg
      181 ggtgttctgc tggtagtggt aggcgagctg cacgccgccg tcctcgatgt tgtggcggat
      241 cttgaagttc gccttgatgc cgttcttctg cttgtcggcc ttgatataga cgttctcgag
      301 tgagctcagc cgacctatag ctctgactgc gtgacctgtc ttattccact tacgacgtga
      361 tgagtcgacc atggtggcga gatccttatc gtcatcgtcg tacagatccc gacccatttg
      421 ctgtccacca gtcatgctag ccataccatg atgatgatga tgatgagaac ccatggtggc
      481 gaattccccc aagcttggaa cgtactaacc ttgatagcta gctagttggc gcgttaagtg
      541 caacacgtga aggccggccg aattcgatct agcttgggct gcaggtcgag ggacctaata
      601 acttcgtata gcatacatta tacgaagtta tattaagggt tccggatcag cttgatgggg
      661 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
      721 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
      781 gcaataaaca agttaacaac aacaattgca ttcattttat gtttt
//
